1. Efficient Intrathymic Gene Transfer Following In Situ Administration of a rAAV Serotype 8 Vector in Mice and Nonhuman Primates.
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Moreau, Aurélie, Vicente, Rita, Dubreil, Laurence, Adjali, Oumeya, Podevin, Guillaume, Jacquet, Chantal, Deschamps, Jack Yves, Klatzmann, David, Cherel, Yan, Taylor, Naomi, Moullier, Philippe, and Zimmermann, Valérie S.
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T cells , *TRANSGENE expression , *LENTIVIRUS diseases , *LABORATORY mice , *THYMIC hormones , *GENETIC transduction - Abstract
The thymus is the primary site of T-cell development and plays a key role in the induction of self-tolerance. We previously showed that the intrathymic (IT) injection of a transgene-expressing lentiviral vector (LV) in mice can result in the correction of a T cell–specific genetic defect. Nevertheless, the efficiency of thymocyte transduction did not exceed 0.1–0.3% and we were unable to detect any thymus transduction in macaques. As such, we initiated studies to assess the capacity of recombinant adeno-associated virus (rAAV) vectors to transduce murine and primate thymic cells. In vivo administration of AAV serotype 2–derived single-stranded AAV (ssAAV) and self-complementary AAV (scAAV) vectors pseudotyped with capsid proteins of serotypes 1, 2, 4, 5, and 8 demonstrated that murine thymus transduction was significantly enhanced by scAAV2/8. Transgene expression was detected in 5% of thymocytes and, notably, transduced cells represented 1% of peripheral T lymphocytes. Moreover, IT administration of scAAV2/8 particles in macaques, by endoscopic-mediated guidance, resulted in significant gene transfer. Thus, in healthy animals, where thymic gene transfer does not provide a selective advantage, scAAV2/8 is a unique tool promoting the in situ transduction of thymocytes with the subsequent export of gene-modified lymphocytes to the periphery.Molecular Therapy (2009) 17 3, 472–479 doi:10.1038/mt.2008.272 [ABSTRACT FROM AUTHOR]
- Published
- 2009
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