1. Identification of Ophiocordyceps sinensis and Its Artificially Cultured Ophiocordyceps Mycelia by Ultra-Performance Liquid Chromatography/Orbitrap Fusion Mass Spectrometry and Chemometrics.
- Author
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Zhang, Ping, Li, Saina, Li, Juan, Wei, Feng, Cheng, Xianlong, Zhang, Guifeng, Ma, Shuangcheng, and Liu, Bin
- Subjects
OPHIOCORDYCIPITACEAE ,LIQUID chromatography ,CHINESE medicine ,AMINO acids ,PEPTIDES - Abstract
Since the cost of
Ophiocordyceps sinensis , an important fungal drug used in Chinese medicine, has increased dramatically, and the counterfeits may have adverse health effects, a rapid and precise marker using the peptide mass spectrometry identification system could significantly enhance the regulatory capacity. In this study, we determined the marker peptides in the digested mixtures of fungal proteins in wildO. sinensis fruiting bodies and various commercially available mycelium fermented powders using ultra-performance liquid chromatography/Orbitrap Fusion mass spectrometry coupled with chemometrics. The results indicated the following marker peptides: TLLEAIDSIEPPK (m /z 713.39) was identified in the wildO. sinensis fruiting body, AVLSDAITLVR (m /z 579.34) was detected in the fermentedO. sinensis mycelium powder, FAELLEK (m /z 849.47) was found in the fermentedOphiocordyceps mycelium powder, LESVVTSFTK (m /z 555.80) was discovered in the artificialOphiocordyceps mycelium powder, and VPSSAVLR (m /z 414.75) was observed inO. mortierella mycelium powder. In order to verify the specificity and applicability of the method, the five marker peptides were synthesized and tested on all samples. All in all, to the best of our knowledge, this is the first time that mass spectrometry has been employed to detect the marker peptides ofO.sinensis and its related products. [ABSTRACT FROM AUTHOR]- Published
- 2018
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