1. Ginsenoside Rb1 Mitigates Escherichia coli Lipopolysaccharide-Induced Endometritis through TLR4-Mediated NF-κB Pathway.
- Author
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Shaukat, Aftab, Shaukat, Irfan, Rajput, Shahid Ali, Shukat, Rizwan, Hanif, Sana, Shaukat, Imran, Zhang, Xinxin, Chen, Chao, Sun, Xuyang, Ye, Tingzhu, Niu, Kaifeng, Yao, Zhiqiu, Shaukat, Shadab, Safdar, Muhammad, Abdelrahman, Mohamed, Riaz, Umair, Zhao, Junwei, Gu, Xiaoying, and Yang, Liguo
- Subjects
ENDOMETRITIS ,ESCHERICHIA coli ,CELLULAR signal transduction ,LABORATORY mice ,GINSENG ,ENDOMETRIUM - Abstract
Endometritis is the inflammatory response of the endometrial lining of the uterus and is associated with low conception rates, early embryonic mortality, and prolonged inter-calving intervals, and thus poses huge economic losses to the dairy industry worldwide. Ginsenoside Rb1 (GnRb1) is a natural compound obtained from the roots of Panax ginseng, having several pharmacological and biological properties. However, the anti-inflammatory properties of GnRb1 in lipopolysaccharide (LPS)-challenged endometritis through the TLR4-mediated NF-κB signaling pathway has not yet been researched. This study was planned to evaluate the mechanisms of how GnRb1 rescues LPS-induced endometritis. In the present research, histopathological findings revealed that GnRb1 ameliorated LPS-triggered uterine injury. The ELISA and RT-qPCR assay findings indicated that GnRb1 suppressed the expression level of pro-inflammatory molecules (TNF-α, IL-1β and IL-6) and boosted the level of anti-inflammatory (IL-10) cytokine. Furthermore, the molecular study suggested that GnRb1 attenuated TLR4-mediated NF-κB signaling. The results demonstrated the therapeutic efficacy of GnRb1 in the mouse model of LPS-triggered endometritis via the inhibition of the TLR4-associated NF-κB pathway. Taken together, this study provides a baseline for the protective effect of GnRb1 to treat endometritis in both humans and animals. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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