14 results on '"Ni, Hui"'
Search Results
2. A Quantitative Analysis Model Established to Determine the Concentration of Each Source in Mixed Astaxanthin from Different Sources
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Du, Xiping, primary, Wang, Xin, additional, Bai, Manli, additional, Liu, Shaosong, additional, Huang, Gaoling, additional, Zhang, Qin, additional, Ni, Hui, additional, and Chen, Feng, additional
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- 2020
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3. Suppressive Interaction Approach for Masking Stale Note of Instant Ripened Pu-Erh Tea Products
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Zhang, Ting, primary, Ni, Hui, additional, Qiu, Xu-Jian, additional, Li, Ting, additional, Zhang, Liang-Zhen, additional, Li, Li-Jun, additional, Jiang, Ze-Dong, additional, Li, Qing-Biao, additional, Chen, Feng, additional, and Zheng, Fu-Ping, additional
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- 2019
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4. In Vivo Pharmacokinetic Analysis Utilizing Non-Targeted and Targeted Mass Spectrometry and In Vitro Assay against Transient Receptor Potential Channels of Maobushisaishinto and Its Constituent Asiasari Radix.
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Matsumoto, Takashi, Takiyama, Mikina, Sanechika, Shou, Nakayama, Akiko, Aoki, Katsuyuki, Ohbuchi, Katsuya, Kushida, Hirotaka, Kanno, Hitomi, Nishi, Akinori, Watanabe, Junko, Xiao, Jianbo, Zhang, Yanbo, and Ni, Hui
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TRP channels ,MASS spectrometry ,RESPIRATORY infections ,PHARMACOKINETICS ,COMMON cold ,AUTORADIOGRAPHY ,INDUCTIVELY coupled plasma mass spectrometry ,LIQUID chromatography-mass spectrometry - Abstract
The Japanese traditional medicine maobushisaishinto (MBST) has been prescribed for treating upper respiratory tract infections, such as a common cold. However, its mode of action is poorly understood, especially concerning the MBST constituent Asiasari Radix (AR). In this study, we focused on AR, with an objective of clarifying its bioavailable active ingredients and role within MBST by performing pharmacokinetic and pharmacological studies. Firstly, we performed qualitative non-targeted analysis utilizing high-resolution mass spectrometry to explore the bioavailable ingredients of AR as well as quantitative targeted analysis to reveal plasma concentrations following oral administration of MBST in rats. Secondly, we performed in vitro pharmacological study of bioavailable AR ingredients in addition to other ingredients of MBST to confirm any agonistic activities against transient receptor potential (TRP) channels. As a result, methyl kakuol and other compounds derived from AR were detected in the rat plasma and showed agonistic activity against TRPA1. This study suggests that methyl kakuol as well as other compounds have the potential to be an active ingredient in AR and thus presumably would contribute in part to the effects exerted by MBST. [ABSTRACT FROM AUTHOR]
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- 2020
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5. In Vitro and In Vivo Screening of Wild Bitter Melon Leaf for Anti-Inflammatory Activity against Cutibacterium acnes.
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Chuang, Lu-Te, Shih, Ya-Hsin, Huang, Wen-Cheng, Lin, Lie-Chwen, Hsu, Chin, Chyuan, Jong-Ho, Tsai, Tsung-Hsien, Tsai, Po-Jung, Xiao, Jianbo, Zhang, Yanbo, and Ni, Hui
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WATERMELONS ,MOMORDICA charantia ,HEXANE ,MITOGEN-activated protein kinases ,INFLAMMATION ,CUTIBACTERIUM acnes ,ETHYL acetate - Abstract
Cutibacterium acnes (formerly Propionibacterium acnes) is a key pathogen involved in the development and progression of acne inflammation. The numerous bioactive properties of wild bitter melon (WBM) leaf extract and their medicinal applications have been recognized for many years. In this study, we examined the suppressive effect of a methanolic extract (ME) of WBM leaf and fractionated components thereof on live C. acnes-induced in vitro and in vivo inflammation. Following methanol extraction of WBM leaves, we confirmed anti-inflammatory properties of ME in C. acnes-treated human THP-1 monocyte and mouse ear edema models. Using a bioassay-monitored isolation approach and a combination of liquid–liquid extraction and column chromatography, the ME was then separated into n-hexane, ethyl acetate, n-butanol and water-soluble fractions. The hexane fraction exerted the most potent anti-inflammatory effect, suppressing C. acnes-induced interleukin-8 (IL-8) production by 36%. The ethanol-soluble fraction (ESF), which was separated from the n-hexane fraction, significantly inhibited C. acnes-induced activation of mitogen-activated protein kinase (MAPK)-mediated cellular IL-8 production. Similarly, the ESF protected against C. acnes-stimulated mouse ear swelling, as measured by ear thickness (20%) and biopsy weight (23%). Twenty-four compounds in the ESF were identified using gas chromatograph–mass spectrum (GC/MS) analysis. Using co-cultures of C. acnes and THP-1 cells, β-ionone, a compound of the ESF, reduced the production of IL-1β and IL-8 up to 40% and 18%, respectively. β-ionone also reduced epidermal microabscess, neutrophilic infiltration and IL-1β expression in mouse ear. We also found evidence of the presence of anti-inflammatory substances in an unfractionated phenolic extract of WBM leaf, and demonstrated that the ESF is a potential anti-inflammatory agent for modulating in vitro and in vivo C. acnes-induced inflammatory responses. [ABSTRACT FROM AUTHOR]
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- 2020
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6. Ten New Dammarane-Type Saponins with Hypolipidemia Activity from a Functional Herbal Tea—Gynostemma pentaphyllum.
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Yin, Maojing, Zhang, Jingjing, Wang, Lizhi, Li, Fangyi, Li, Zhenfa, Xiang, Wei, Bie, Songtao, Wang, Chunhua, Li, Zheng, Xiao, Jianbo, Zhang, Yanbo, and Ni, Hui
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GYNOSTEMMA pentaphyllum ,SAPONINS ,HERBAL teas ,BLOOD lipids ,OLEIC acid ,HEPATOCELLULAR carcinoma ,CHEMICAL structure - Abstract
Gynostemma pentaphyllum (thumb.) Makino is a functional herbal tea commonly used in Asian countries and regions to reduce blood lipid levels. G. pentaphyllum saponin is the main component, but there are still a large number of components with lipid-lowering activity that have not been found. In this study, 10 novel dammarane-type saponins, (1–10) and a known one (11) were isolated from G. pentaphyllum. Ten new compounds were identified and named as yunnangypenosides A-J (1–10), and another known one (11) was also obtained. Their chemical structures were determined by MS, NMR spectroscopic analyses. Moreover, the cytotoxicities on human HepG-2 hepatocellular carcinoma cells of these isolates were evaluated, and the results showed that compounds 1–11 had no obvious cytotoxicity. Finally, all these compounds were evaluated for their lipid-lowering effect by means of the oil red O staining method. Ten compounds could significantly reduce lipid levels except of 2, especially 8 exhibite the strongest hypolipidemia activity. [ABSTRACT FROM AUTHOR]
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- 2020
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7. Comparison of Phytochemicals, Antioxidant, and In Vitro Anti-Alzheimer Properties of Twenty-Seven Morus spp. Cultivated in Thailand.
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Temviriyanukul, Piya, Sritalahareuthai, Varittha, Jom, Kriskamol Na, Jongruaysup, Butsara, Tabtimsri, Somying, Pruesapan, Kanchana, Thangsiri, Sirinapa, Inthachat, Woorawee, Siriwan, Dalad, Charoenkiatkul, Somsri, Suttisansanee, Uthaiwan, Xiao, Jianbo, Zhang, Yanbo, and Ni, Hui
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MULBERRY ,ABSTRACTION reactions ,PHYTOCHEMICALS ,CHARGE exchange ,ALZHEIMER'S disease ,ANTIOXIDANTS ,FRUIT extracts - Abstract
Alzheimer's disease (AD) is a progressive neurodegenerative disorder. To fight the disease, natural products, including mulberry, with antioxidant activities and inhibitory activities against key enzymes (acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and beta-secretase 1 (BACE-1)) are of interest. However, even in the same cultivars, mulberry trees grown in different populated locations might possess disparate amounts of phytochemical profiles, leading to dissimilar health properties, which cause problems when comparing different cultivars of mulberry. Therefore, this study aimed to comparatively investigate the phytochemicals, antioxidant activities, and inhibitory activities against AChE, BChE, and BACE-1, of twenty-seven Morus spp. cultivated in the same planting area in Thailand. The results suggested that Morus fruit samples were rich in phenolics, especially cyanidin, kuromanin, and keracyanin. Besides, the aqueous Morus fruit extracts exhibited antioxidant activities, both in single electron transfer (SET) and hydrogen atom transfer (HAT) mechanisms, while strong inhibitory activities against AD key enzymes were observed. Interestingly, among the twenty-seven Morus spp., Morus sp. code SKSM 810191 with high phytochemicals, antioxidant activities and in vitro anti-AD properties is a promising cultivar for further developed as a potential mulberry resource with health benefits against AD. [ABSTRACT FROM AUTHOR]
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- 2020
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8. Antiobesity Effects of Gentiana lutea Extract on 3T3-L1 Preadipocytes and a High-Fat Diet-Induced Mouse Model.
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Park, Eunkuk, Lee, Chang Gun, Kim, Junho, Yeo, Subin, Kim, Ji Ae, Choi, Chun Whan, Jeong, Seon-Yong, Xiao, Jianbo, Zhang, Yanbo, and Ni, Hui
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MESSENGER RNA ,GENTIANA ,ATP-binding cassette transporters ,LEPTIN ,GLUCOSE transporters ,METABOLIC disorders ,METABOLIC syndrome - Abstract
Obesity is one of the most common metabolic diseases resulting in metabolic syndrome. In this study, we investigated the antiobesity effect of Gentiana lutea L. (GL) extract on 3T3-L1 preadipocytes and a high-fat-diet (HFD)-induced mouse model. For the induction of preadipocytes into adipocytes, 3T3-L1 cells were induced by treatment with 0.5 mM 3-isobutyl-1-methylxanthine, 1 mM dexamethasone, and 1 μg/mL insulin. Adipogenesis was assessed based on the messenger ribonucleic acid expression of adipogenic-inducing genes (adiponectin (Adipoq), CCAAT/enhancer-binding protein alpha (Cebpa), and glucose transporter type 4 (Slc2a4)) and lipid accumulation in the differentiated adipocytes was visualized by Oil Red O staining. In vivo, obese mice were induced with HFD and coadministered with 100 or 200 mg/kg/day of GL extract for 12 weeks. GL extract treatment inhibited adipocyte differentiation by downregulating the expression of adipogenic-related genes in 3T3-L1 cells. In the obese mouse model, GL extract prevented HFD-induced weight gain, fatty hepatocyte deposition, and adipocyte size by decreasing the secretion of leptin and insulin. In conclusion, GL extract shows antiobesity effects in vitro and in vivo, suggesting that this extract can be beneficial in the prevention of obesity. [ABSTRACT FROM AUTHOR]
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- 2020
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9. Enzyme and Biological Activities of the Water Extracts from the Plants Aesculus hippocastanum, Olea europaea and Hypericum perforatum That Are Used as Folk Remedies in Turkey.
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Sarikurkcu, Cengiz, Locatelli, Marcello, Tartaglia, Angela, Ferrone, Vincenzo, Juszczak, Aleksandra M., Ozer, Mehmet Sabih, Tepe, Bektas, Tomczyk, Michał, Xiao, Jianbo, Zhang, Yanbo, and Ni, Hui
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HYPERICUM perforatum ,TRADITIONAL medicine ,SAPONINS ,TANDEM mass spectrometry ,PLANT extracts ,METABOLITES ,OLIVE - Abstract
Phenolic compounds are secondary metabolites that are found ubiquitously in plants, fruits, and vegetables. Many studies have shown that regular consumption of these compounds could have a positive effect on our health. The aim of this study was to compare the phytochemical contents of the water extracts from three different plants used as folk remedies in Turkey: Aesculus hippocastanum, Olea europaea, and Hypericum perforatum. A liquid chromatography-electrospray tandem mass spectrometry (LC-ESI-MS/MS) analysis was performed to explore the phenolic profiles. The biological activities of these extracts were also evaluated in terms of their antioxidant activities (2,2-diphenyl-1-picrylhydrazyl DPPH, 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid ABTS, Ferric Reducing Antioxidant Power Assay FRAP, cupric ion reducing antioxidant capacity CUPRAC, β-carotene, phosphomolybdenum, and metal chelating) and enzyme inhibitory properties (against acetylcholinesterase, butyrylcholinesterase, and tyrosinase). The aqueous extract of H. perforatum showed the highest levels of total phenolic, flavonoid, and saponin contents. Protocatechuic acid, vanillic acid, verbascoside, hesperidin, hyperoside, apigenin 7-hexosides, and quercetin were the most common compounds found in this species. The results confirm that A. hippocastanum, O. europaea, and H. perforatum represent a potential source of natural-derived molecules with positive properties that could be used as valid starting point for new food supplements, and drugs in the pharmaceutical, cosmetic, and food industries. [ABSTRACT FROM AUTHOR]
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- 2020
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10. Physicochemical and Digestion Properties of Potato Starch Were Modified by Complexing with Grape Seed Proanthocyanidins.
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Zhang, Zirui, Tian, Jinhu, Fang, Haitian, Zhang, Huiling, Kong, Xiangli, Wu, Dongmei, Zheng, Jiaqi, Liu, Donghong, Ye, Xingqian, Chen, Shiguo, Xiao, Jianbo, Zhang, Yanbo, and Ni, Hui
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STARCH ,GRAPE seeds ,FRENCH fries ,DIGESTION ,PROANTHOCYANIDINS ,FOURIER transform infrared spectroscopy ,TYPE 2 diabetes ,DIGESTIVE enzymes - Abstract
Dietary intake of potato starch could induce a dramatic increase in blood glucose and is positively associated with chronic metabolic diseases (type II diabetes, cardiovascular disease, etc.). Grape seed proanthocyanidins (GSP) are known to decrease starch digestion by inhibiting digestive enzymes or changing the physicochemical properties of starch. In the present study, GSP were complexed with potato starch to prepare polyphenol–starch complexes. The physiochemical properties and digestibility of complexes were investigated by in vitro digestion model, X-ray diffraction, differential scanning calorimetry, rapid visco analyzer, Fourier transform infrared spectroscopy as well as texture profile analysis. Results indicated that the peak viscosity, breakdown, trough, and setback of the complexes disappeared, replaced by a special continuous increase in paste viscosity. The complexes showed a lower final viscosity and higher thermal stability with the increasing binding amount of GSP. GSP decreased the hardness of the complexes' gel significantly. FT-IR indicated that GSP might interact with potato starch through noncovalent forces. Additionally, the complexes also showed a higher content of slowly digestible starch and resistant starch than that of the native starch. Thus, we inferred that the addition of GSP could modify the digestibility of potato starch and be an optional way to modify the starch with lower digestion. [ABSTRACT FROM AUTHOR]
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- 2020
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11. Gamma Irradiated Rhodiola sachalinensis Extract Ameliorates Testosterone-Induced Benign Prostatic Hyperplasia by Downregulating 5-Alpha Reductase and Restoring Testosterone in Rats.
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Xin, Qi, Kwon, Mi-Jin, Lee, Ju-Woon, Kim, Kwan-Soo, Chen, Hao, Campos, Maria G., Tundis, Rosa, Cui, Cheng-Bi, Cho, Young Ho, Cao, Hui, Xiao, Jianbo, Zhang, Yanbo, and Ni, Hui
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BENIGN prostatic hyperplasia ,TESTOSTERONE ,GAMMA rays ,TRANSFORMING growth factors-beta ,EXOCRINE glands ,RATS ,SUBCUTANEOUS injections ,KERATINIZATION - Abstract
The effect of Rhodiola sachalinensis Boriss extract irradiated with 50 kGy gamma rays (HKC) on benign prostatic hyperplasia (BPH) was investigated. Seven-week-old male SD rats received a subcutaneous injection of 20 mg/kg of testosterone propionate (TP) to induce BPH. Then, the testosterone only group received testosterone, the testosterone + finasteride group received testosterone and finasteride (5 mg/kg), the testosterone + HKC group received testosterone and HKC extract (500 mg/kg). Prostate weight and the dihydrotestosterone (DHT) levels in serum or prostate tissue were determined. The mRNA expressions of 5-alpha reductase (AR) in prostate tissue were also measured. Compared to the control group, prostate weight was significantly improved in the TP group and decreased in the HKC and finasteride-treated groups. Furthermore, the mRNA expression of 5-AR in the prostate was significantly reduced in the HKC and finasteride-treated groups. Similarly, the expression levels of α-smooth muscle actin (α-SMA) and cytokeratin, which are associated with prostatic enlargement in the HKC and finasteride groups, were much lower than in the TP group. HKC treatment showed similar efficacy to finasteride treatment on rats with testosterone-induced BPH. HKC may be explored as a potential new drug for BPH treatment. [ABSTRACT FROM AUTHOR]
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- 2019
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12. Hydrophobically Modified Glucan as an Amphiphilic Carbohydrate Polymer for Micellar Delivery of Myricetin.
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Yang, Weiyu, Guo, Ling, Li, Fenfen, Liu, Xin, Nie, Shaoping, Xie, Mingyong, Huang, Danfei, Xiao, Jianbo, Zhang, Yanbo, and Ni, Hui
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MYRICETIN ,CRITICAL micelle concentration ,POLYMERS ,GLUCANS ,TRANSMISSION electron microscopes ,HYDROPHOBIC compounds - Abstract
Myricetin (Myr) is a phytochemical with many functional properties. However, its hydrophobicity, low bioavailability, and stability limit its application. In this study, octadecanoate oat β-glucan (OGE) was synthesized and gained recognition as a self-assembled micelle forming a polymer with a critical micelle concentration (CMC) of 59.4 μg/mL. The Myr-loaded OGE micelle was then prepared and characterized by dynamic light scattering (DLS), transmission electron microscope (TEM), X-ray diffractometer (XRD), and Fourier-transform infrared spectroscopy (FT-IR) spectra. The water solubility of Myr was greatly enhanced by forming the Myr/OGE inclusion complex. Consequently, compared to free Myr, the retention of Myr in Myr-loaded OGE micelle was effectively increased during the intestinal digestion phase, and its antioxidant activity was also improved. Overall, our findings demonstrated the potential applications of OGE polymer for the development of prospective micelle in health food, cosmetics, and pharmaceutical fields because they can aid in the delivery of hydrophobic functional compounds like Myr. [ABSTRACT FROM AUTHOR]
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- 2019
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13. Phytochemical Study of Stem and Leaf of Clausena lansium.
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Peng, Wenwen, Fu, Xiaoxiang, Li, Yuyan, Xiong, Zhonghua, Shi, Xugen, Zhang, Fang, Huo, Guanghua, Li, Baotong, Xiao, Jianbo, Zhang, Yanbo, and Ni, Hui
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PHYTOCHEMICALS ,GLYCOSIDES ,TRADITIONAL medicine ,LEAVES ,ADENOSINES ,RUTACEAE ,ALKALOIDS - Abstract
Clausena lansium Lour. Skeels (Rutaceae) is widely distributed in South China and has historically been used as a traditional medicine in local healthcare systems. Although the characteristic components (carbazole alkaloids and coumarins) of C. lansium have been found to possess a wide variety of biological activities, little attention has been paid toward the other components of this plant. In the current study, phytochemical analysis of isolates from a water-soluble stem and leaf extract of C. lansium led to the identification of 12 compounds, including five aromatic glycosides, four sesquiterpene glycosides, two dihydrofuranocoumarin glycosides, and one adenosine. All compounds were isolated for the first time from the genus Clausena, including a new aromatic glycoside (1), a new dihydrofuranocoumarin glycoside (6), and two new sesquiterpene glycosides (8 and 9). The phytochemical structures of the isolates were elucidated using spectroscopic analyses including NMR and MS. The existence of these compounds demonstrates the taxonomic significance of C. lansium in the genus Clausena and suggests that some glycosides from this plant probably play a role in the anticancer activity of C. lansium to some extent. [ABSTRACT FROM AUTHOR]
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- 2019
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14. Involvement of Mitochondrial Dysfunction, Endoplasmic Reticulum Stress, and the PI3K/AKT/mTOR Pathway in Nobiletin-Induced Apoptosis of Human Bladder Cancer Cells.
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Goan, Yih-Gang, Wu, Wen-Tung, Liu, Chih-I, Neoh, Choo-Aun, Wu, Yu-Jen, Xiao, Jianbo, Zhang, Yanbo, and Ni, Hui
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CANCER cells ,ENDOPLASMIC reticulum ,BLADDER cancer ,WESTERN immunoblotting ,APOPTOSIS ,CELL death - Abstract
Nobiletin (NOB) is a polymethoxylated flavonoid isolated from citrus fruit peel that has been shown to possess anti-tumor, antithrombotic, antifungal, anti-inflammatory and anti-atherosclerotic activities. The main purpose of this study was to explore the potential of using NOB to induce apoptosis in human bladder cancer cells and study the underlying mechanism. Using an MTT assay, agarose gel electrophoresis, a wound-healing assay, flow cytometry, and western blot analysis, this study investigated the signaling pathways involved in NOB-induced apoptosis in BFTC human bladder cancer cells. Our results showed that NOB at concentrations of 60, 80, and 100 μM inhibited cell growth by 42%, 62%, and 80%, respectively. Cells treated with 60 μM NOB demonstrated increased DNA fragmentation, and flow cytometry analysis confirmed that the treatment caused late apoptotic cell death. Western blot analysis showed that mitochondrial dysfunction occurred in NOB-treated BFTC cells, leading to cytochrome C release into cytosol, activation of pro-apoptotic proteins (caspase-3, caspase-9, Bad, and Bax), and inhibition of anti-apoptotic proteins (Mcl-1, Bcl-xl, and Bcl-2). NOB-induced apoptosis was also mediated by regulating endoplasmic reticulum stress via the PERK/elF2α/ATF4/CHOP pathway, and downregulating the PI3K/AKT/mTOR pathway. Our results suggested that the cytotoxic and apoptotic effects of NOB on bladder cancer cells are associated with endoplasmic reticulum stress and mitochondrial dysfunction. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
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