1. Characterization of a Flavonoid 3'/5'/7- O -Methyltransferase from Citrus reticulata and Evaluation of the In Vitro Cytotoxicity of Its Methylated Products.
- Author
-
Liu X, Wang Y, Chen Y, Xu S, Gong Q, Zhao C, Cao J, and Sun C
- Subjects
- Cell Line, Tumor, Cell Survival drug effects, Citrus chemistry, Cytotoxins chemistry, Cytotoxins isolation & purification, Epithelial Cells drug effects, Epithelial Cells pathology, Flavones chemistry, Flavones isolation & purification, Fruit chemistry, Fruit enzymology, Humans, Inhibitory Concentration 50, Methylation, Plant Proteins isolation & purification, Protein O-Methyltransferase isolation & purification, Substrate Specificity, Citrus enzymology, Cytotoxins pharmacology, Flavones pharmacology, Plant Proteins chemistry, Protein O-Methyltransferase chemistry
- Abstract
O -methylation of flavonoids is an important modification reaction that occurs in plants. O -methylation contributes to the structural diversity of flavonoids, which have several biological and pharmacological functions. In this study, an O -methyltransferase gene ( CrOMT2 ) was isolated from the fruit peel of Citrus reticulata , which encoding a multifunctional O -methyltransferase and could effectively catalyze the methylation of 3'-, 5'-, and 7-OH of flavonoids with vicinal hydroxyl substitutions. Substrate preference assays indicated that this recombinant enzyme favored polymethoxylated flavones (PMF)-type substrates in vitro, thereby providing biochemical evidence for the potential role of the enzyme in plants. Additionally, the cytotoxicity of the methylated products from the enzymatic catalytic reaction was evaluated in vitro using human gastric cell lines SGC-7901 and BGC-823. The results showed that the in vitro cytotoxicity of the flavonoids with the unsaturated C2-C3 bond was increased after being methylated at position 3'. These combined results provide biochemical insight regarding CrOMT2 in vitro and indicate the in vitro cytotoxicity of the products methylated by its catalytic reaction.
- Published
- 2020
- Full Text
- View/download PDF