1. Region Met225 to Ile412 of Bacillus cereus Hemolysin II Is Capable to Agglutinate Red Blood Cells.
- Author
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Nagel AS, Rudenko NV, Luchkina PN, Karatovskaya AP, Zamyatina AV, Andreeva-Kovalevskaya ZI, Siunov AV, Brovko FA, and Solonin AS
- Subjects
- Animals, Rabbits, Bacterial Proteins chemistry, Erythrocytes metabolism, Antibodies, Monoclonal metabolism, Bacillus cereus metabolism, Hemolysin Proteins chemistry
- Abstract
Hemolysin II (HlyII) is one of the virulence factors of the opportunistic bacterium Bacillus cereus belonging to the group of β-pore-forming toxins. This work created a genetic construct encoding a large C-terminal fragment of the toxin (HlyIILCTD, M225-I412 according to the numbering of amino acid residues in HlyII). A soluble form of HlyIILCTD was obtained using the SlyD chaperone protein. HlyIILCTD was first shown to be capable of agglutinating rabbit erythrocytes. Monoclonal antibodies against HlyIILCTD were obtained by hybridoma technology. We also proposed a mode of rabbit erythrocyte agglutination by HlyIILCTD and selected three anti-HlyIILCTD monoclonal antibodies that inhibited the agglutination.
- Published
- 2023
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