1. Heterogeneous expression and emulsifying activity of class I hydrophobin from Pholiota nameko.
- Author
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Kudo, Takunobu, Sato, Yuki, Tasaki, Yuji, Hara, Takashi, and Joh, Toshio
- Subjects
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PHOLIOTA , *HYDROPHOBINS , *FILAMENTOUS fungi , *BASIDIOMYCETES , *EMULSIONS , *GLUTATHIONE transferase , *ESCHERICHIA coli , *SOLUBILITY - Abstract
Hydrophobins secreted by filamentous fungi self-assemble into an amphipathic film at hydrophilic/hydrophobic interfaces. This unique property suggests that the hydrophobins have a high potential for industrial applications. However, the assemblages of class I hydrophobins are highly insoluble, making such commercial applications difficult. To enhance the solubility of class I hydrophobins, we have attempted to express class I hydrophobin PNH1 from Pholiota nameko fused with glutathione S-transferase (GST) in Escherichia coli. The GST-PNH1 was effectively isolated from the soluble fraction of transformed E. coli, and subsequent analysis revealed that the purified GST-PNH1 had almost the same emulsifying activity as PNH1. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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