1. Scedosporium and Pseudallescheria low molecular weight metabolites revealed by database search
- Author
-
Jean-Philippe Bouchara, Karel Lemr, Miroslav Sulc, Martin Strohalm, Vladimír Havlíček, Eliana Barreto-Bergter, and Lukas Krasny
- Subjects
0303 health sciences ,Chromatography ,biology ,Scedosporium prolificans ,030306 microbiology ,010401 analytical chemistry ,Scedosporium apiospermum ,Pseudallescheria ,Dermatology ,General Medicine ,Tandem mass spectrometry ,Mass spectrometry ,biology.organism_classification ,01 natural sciences ,0104 chemical sciences ,Microbiology ,Scedosporium ,Pseudallescheria boydii ,03 medical and health sciences ,Infectious Diseases ,Metabolomics - Abstract
The potential of mMass software search tool with new compound libraries was demonstrated on metabolomics of Scedosporium prolificans, S. apiospermum and Pseudallescheria boydii sensu stricto. Cyclic peptides pseudacyclins, small molecular weight tyroscherin analogues and various lipids were annotated by public software tool (http://www.mmass.org) utilising accurate matrix-assisted laser desorption/ionisation mass spectral data of intact fungal spores. Electrospray ionisation combined with tandem mass spectrometry was used for monohexosylceramide characterisation in fungal extracts.
- Published
- 2011