1. Targeting pancreatic cancer with magneto-fluorescent theranostic gold nanoshells.
- Author
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Chen W, Ayala-Orozco C, Biswal NC, Perez-Torres C, Bartels M, Bardhan R, Stinnet G, Liu XD, Ji B, Deorukhkar A, Brown LV, Guha S, Pautler RG, Krishnan S, Halas NJ, and Joshi A
- Subjects
- Acute-Phase Proteins metabolism, Animals, Cell Line, Tumor, Contrast Media chemistry, Contrast Media therapeutic use, Drug Delivery Systems, Female, Fluorescent Dyes chemistry, Fluorescent Dyes therapeutic use, Gold chemistry, Humans, Hyperthermia, Induced, Lipocalin-2, Lipocalins metabolism, Magnetic Resonance Imaging, Magnets chemistry, Mice, Nude, Nanoshells chemistry, Oncogene Proteins metabolism, Optical Imaging, Pancreatic Neoplasms pathology, Phototherapy, Gold therapeutic use, Nanoshells therapeutic use, Pancreas pathology, Pancreatic Neoplasms diagnosis, Pancreatic Neoplasms therapy
- Abstract
Aim: We report a magneto-fluorescent theranostic nanocomplex targeted to neutrophil gelatinase-associated lipocalin (NGAL) for imaging and therapy of pancreatic cancer., Materials & Methods: Gold nanoshells resonant at 810 nm were encapsulated in silica epilayers doped with iron oxide and the near-infrared (NIR) dye indocyanine green, resulting in theranostic gold nanoshells (TGNS), which were subsequently conjugated with antibodies targeting NGAL in AsPC-1-derived xenografts in nude mice., Results: Anti-NGAL-conjugated TGNS specifically targeted pancreatic cancer cells in vitro and in vivo providing contrast for both NIR fluorescence and T2-weighted MRI with higher tumor contrast than can be obtained using long-circulating, but nontargeted, PEGylated nanoparticles. The nanocomplexes also enabled highly specific cancer cell death via NIR photothermal therapy in vitro., Conclusion: TGNS with embedded NIR and magnetic resonance contrasts can be specifically targeted to pancreatic cancer cells with expression of early disease marker NGAL, and enable molecularly targeted imaging and photothermal therapy.
- Published
- 2014
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