1. Role of purinergic receptors in platelet-nanoparticle interactions
- Author
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Chatterjee Mohor, Om Parkash Siwach, Anjan Kr. Dasgupta, Prabir Lahiri, Prasenjit Sen, Sankar Pal Choudhuri, Jaydeep Bhattacharya, Soumitra Kar, Suryyani Deb, and Utpal Chaudhuri
- Subjects
Purinergic receptor ,Biomedical Engineering ,chemistry.chemical_element ,Nanoparticle ,Laser Doppler velocimetry ,Toxicology ,Copper ,Cadmium sulfide ,Adenosine diphosphate ,chemistry.chemical_compound ,chemistry ,Dynamic light scattering ,Biochemistry ,Biophysics ,Platelet - Abstract
Primary objective. Elevation of the thrombotic responses mediated by a variety of carbon-derived nanoparticles was recently reported in the literature. In this paper our objective was to investigate whether metal nanoparticles (iron, copper, gold or cadmium sulfide [CdS]) impart such prothrombotic effects on human platelets. Secondly, we wanted to examine whether such effects were mediated through any specific platelet receptor. Experimental design. The size distributions and zeta potentials of characterized gold, copper, iron and CdS (rod & sphere) nanoparticles were measured using photon correlation spectroscopy and laser Doppler velocimetry. The effect of two classes of agonists, adenosine diphosphate (ADP) and epinephrine were studied. To study the effect of ADP, a suboptimal concentration was chosen below a critical concentration. Above the critical concentration, the aggregation assumed its standard hyperbolic shape (and de-aggregation disappeared). Pro-aggregatory action of a given agent can be und...
- Published
- 2007