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Aspergillus flavusand Aspergillus parasiticusare the major contaminants known to produce aflatoxins in food crops (maize, cotton, corn, peanuts and oil-seed). Nowadays various modified cultural practices are being utilized and explored to overcome food contamination problem. The current study was aimed to evaluate the effect of phytochemicals (Ascorbic acid, Gallic acid, Caffeine and Quercetin) on toxigenic strains of A. flavus(MTCC 11866) and A. parasiticus(MTCC 8189) by Poisoned food plate technique and MTT assay (3-(4,5-Dimethylthiazole-2-yl)-2,5-Diphenyltetrazolium bromide). In comparison with control (Aspergillusspores without phytochemicals) a significant decrease in growth was observed with all the compounds. Quercetin showed strongest inhibitory effect with MIC50at 36 µg/ml in A. parasiticusand MIC50at 113 µg/ml in A. flavus. Caffeine showed good inhibitory effect in A. parasiticus(160 µg/ml) whereas Gallic acid showed good inhibitory effect in A. flavus(153 µg/ml). Ascorbic acid showed least effect among phytochemicals tested in both the Aspergillusspecies (322 µg/ml).