1. Improvement in phloroglucinol compound extracted from different parts of Eugenia umbelliflora monitored by LC-UV and antimicrobial activity.
- Author
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Farias IV, Amorim CM, Graff E, Breia I, Dos Santos MC, Norberto S, Faqueti LG, Bresolin TMB, Bella Cruz A, and Meyre-Silva C
- Subjects
- Ethanol, Phloroglucinol pharmacology, Plant Extracts pharmacology, Solvents, Staphylococcus aureus, Anti-Infective Agents pharmacology, Eugenia
- Abstract
The phloroglucinol eugenial C, eugenial D and eugenial E are the main active compounds in Eugenia umbelliflora fruits. This study aims to evaluate the extraction conditions of E. umbelliflora , using ethanol as solvent, focusing on the phloroglucinol and antimicrobial activity. In order to optimize the extraction conditions, ethanol 50, 70 and 90
o GL was used as a solvent in the proportions of 1:20 (w/v) of drug:solvent ratio (D:S), stirring (330 rpm) at room temperature during 4 h, monitored by LC-UV and antimicrobial assay. The LC-UV method developed was linear over a concentration range of 3.4-68.0, 5.3-106.0 and 5.0-100.8 μg.mL-1 of eugenial C, eugenial D and eugenial E, having LOQ of 1.68, 1.33 and 0.8 μg.mL-1 , respectively. The fruits showed the best herbal raw material and showed the highest phloroglucinol concentration and activity against S. aureus , when extracted with ethanol 90o GL, during 4 h, at 1:20 of D:S.- Published
- 2022
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