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An hydroxytyrosol (HTyr) enriched extract (HTE), obtained from olive mill wastewaters (OMWW) through an eco-friendly, patented process was tested on two olive tree pathogens, Pseudomonas savastanoi pv. savastanoi (Pss) and Agrobacterium tumefaciens (At). Pss, in particular, is a feared treat for olive tree cultivation being the etiological agent of the olive knot disease responsible of severe production losses. Chemical characterization allowed to identify and quantify HTyr as the main constituent along with other low molecular weight phenols. HTE has proven potent antioxidant activity and significant antimicrobial activity against Pss and At in vitro, in both cases higher than hydroxytyrosol alone, suggesting an important role also of the minor phenolic components, which act synergistically with HTyr. Alternatives to the molecules currently present on the market are needed, but only the use of standardized extraction technologies would allow to conduct solid field studies and enable this circular approach to find a real application in the olive groves.

Hydroalcoholic extracts obtained from buds of

An hydroxytyrosol (HTyr) enriched extract (HTE), obtained from olive mill wastewaters (OMWW) through an eco-friendly, patented process was tested on two olive tree pathogens

Natural Product Research, DOI: 10.1080/14786419.2018.1508149 Antioxidant and anti-inflammatory effects of pomegranate peel extracts on bovine mammary epithelial cells BME-UV1 Fabio Mastrogiovanni a, Roberta Bernini a, Loredana Basiricò a,Umberto Bernabucci a , Margherita Campo b, Annalisa Romani b, Luca Santi a and Nicola Lacetera a aDepartment of Agricultural and Forestry Sciences (DAFNE), University of Tuscia, Viterbo, Italy; bDepartment of Statistics, Computing, Applications “Giuseppe Parenti” DiSIA, Phytolab, University of Florence, Florence, Italy Pomegranate peel extracts (PPE) were tested for the first time on BME-UV1, a valid cellular model to study the bovine mammary epithelial metabolism, to evaluate the effects on the oxidative stress and inflammatory status. Based on the statistical analysis of MTT data, PPE at 0.1, 1.0 and 10 lg/mL resulted not cytotoxic after 24 h, 48 h and 7 days of treatment. At the same concentrations, PPE induced a reduction of ROS production elicited by the addition of hydrogen peroxide or lipopolysaccharide evidencing an antioxidant effect confirmed also by a decrease of malondialdehyde. At 10 lg/mL, PPE reduced pro-inflammatory cytokines expressions showing an anti-inflammatory effect on BME-UV1 treated with lipopolysaccharide. Although in vivo experiments are necessary, the results of this study are promising for future applications of PPE as feed supplement for dairy cattle, in particular around calving, when the animals are more subject to oxidative stress and inflammatory diseases.