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Your search keyword '"Bhattacharya, S K"' showing total 9 results
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9. A mammalian protein with specific demethylase activity for mCpG DNA.

Author

Bhattacharya SK, Ramchandani S, Cervoni N, and Szyf M

Subjects
Amino Acid Sequence, Animals, Catalysis, Cells, Cultured, Cloning, Molecular, Cytosine metabolism, DNA Methylation, DNA, Complementary, HeLa Cells, Humans, Methanol metabolism, Mice, Molecular Sequence Data, Oxidoreductases, O-Demethylating genetics, Oxidoreductases, O-Demethylating metabolism, Sequence Homology, Amino Acid, Substrate Specificity, Transfection, Tumor Cells, Cultured, DNA metabolism, Dinucleoside Phosphates metabolism, Oxidoreductases, O-Demethylating isolation & purification
Abstract

DNA-methylation patterns are important for regulating genome functions, and are determined by the enzymatic processes of methylation and demethylation. The demethylating enzyme has now been identified: a mammalian complementary DNA encodes a methyl-CpG-binding domain, bears a demethylase activity that transforms methylated cytosine bases to cytosine, and demethylates a plasmid when the cDNA is translated or transiently transfected into human embryonal kidney cells in vitro. The discovery of this DNA demethylase should provide a basis for the molecular and developmental analysis of the role of DNA methylation and demethylation.

Published
1999
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