1. Direct activation of RNA polymerase III transcription by c-Myc
- Author
-
Carla Grandori, Natividad Gomez-Roman, Robert J. White, and Robert N. Eisenman
- Subjects
Transcriptional Activation ,Transcription, Genetic ,RNA polymerase II ,Proto-Oncogene Mas ,RNA polymerase III ,Cell Line ,Substrate Specificity ,Proto-Oncogene Proteins c-myc ,RNA, Transfer ,Transcription Factor TFIIIB ,Cyclins ,Transcriptional regulation ,RNA polymerase I ,Animals ,Cyclin D2 ,Humans ,RNA, Messenger ,RNA polymerase II holoenzyme ,Multidisciplinary ,General transcription factor ,biology ,RNA, Ribosomal, 5S ,RNA Polymerase III ,DNA ,Fibroblasts ,Precipitin Tests ,Molecular biology ,Chromatin ,Protein Structure, Tertiary ,Rats ,Enzyme Activation ,biology.protein ,RNA Polymerase II ,Transcription factor II D ,Cell Division ,Gene Deletion ,Protein Binding - Abstract
The proto-oncogene product c-Myc has a direct role in both metazoan cell growth and division. RNA polymerase III (pol III) is involved in the generation of transfer RNA and 5S ribosomal RNA, and these molecules must be produced in bulk to meet the need for protein synthesis in growing cells. We demonstrate here that c-Myc binds to TFIIIB, a pol III-specific general transcription factor, and directly activates pol III transcription. Chromatin immunoprecipitation reveals that endogenous c-Myc is present at tRNA and 5S rRNA genes in cultured mammalian cells. These results suggest that activation of pol III may have a role in the ability of c-Myc to stimulate cell growth.
- Published
- 2003