1. The cell-surface proteoglycan Dally regulates Wingless signalling in Drosophila.
- Author
-
Tsuda M, Kamimura K, Nakato H, Archer M, Staatz W, Fox B, Humphrey M, Olson S, Futch T, Kaluza V, Siegfried E, Stam L, and Selleck SB
- Subjects
- Animals, Animals, Genetically Modified, Cloning, Molecular, Drosophila genetics, Epidermis embryology, Epidermis physiology, Female, Genes, Insect, Genetic Techniques, Genitalia embryology, Glycosylphosphatidylinositols chemistry, Glycosylphosphatidylinositols physiology, Heparan Sulfate Proteoglycans chemistry, Heparan Sulfate Proteoglycans physiology, Homeodomain Proteins physiology, Insect Proteins physiology, Larva chemistry, Male, Membrane Glycoproteins chemistry, Membrane Glycoproteins genetics, Mutation, Proteoglycans chemistry, Proteoglycans genetics, RNA metabolism, Transcription Factors physiology, Wnt1 Protein, Drosophila physiology, Drosophila Proteins, Membrane Glycoproteins physiology, Proteoglycans physiology, Proto-Oncogene Proteins physiology, Signal Transduction
- Abstract
Wingless (Wg) is a member of the Wnt family of growth factors, secreted proteins that control proliferation and differentiation during development. Studies in Drosophila have shown that responses to Wg require cell-surface heparan sulphate, a glycosaminoglycan component of proteoglycans. These findings suggest that a cell-surface proteoglycan is a component of a Wg/Wnt receptor complex. We demonstrate here that the protein encoded by the division abnormally delayed (dally) gene is a cell-surface, heparan-sulphate-modified proteoglycan. dally partial loss-of-function mutations compromise Wg-directed events, and disruption of dally function with RNA interference produces phenotypes comparable to those found with RNA interference of wg or frizzled (fz)/Dfz2. Ectopic expression of Dally potentiates Wg signalling without altering levels of Wg and can rescue a wg partial loss-of-function mutant. We also show that dally, a regulator of Decapentaplegic (Dpp) signalling during post-embryonic development, has tissue-specific effects on Wg and Dpp signalling. Dally can therefore differentially influence signalling mediated by two growth factors, and may form a regulatory component of both Wg and Dpp receptor complexes.
- Published
- 1999
- Full Text
- View/download PDF