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Your search keyword '"Voigt, Christopher A"' showing total 8 results
Start Over Author "Voigt, Christopher A" Journal nature biotechnology
8 results on '"Voigt, Christopher A"'

5. Automated Design of Synthetic Ribosome Binding Sites to Precisely Control Protein Expression

Author

Salis, Howard M., Mirsky, Ethan A., and Voigt, Christopher A.

Subjects
Ribosomal Proteins, Binding Sites, Escherichia coli Proteins, DNA, Recombinant, translation, Reproducibility of Results, RNA secondary structure, Article, Protein Biosynthesis, Escherichia coli, Thermodynamics, synthetic biology, genetic circuit, RNA, Messenger, Cloning, Molecular, metabolic engineering, Genetic Engineering, optimization, Ribosomes, Algorithms
Abstract

Microbial engineering often requires fine control over protein expression--for example, to connect genetic circuits or control flux through a metabolic pathway. To circumvent the need for trial and error optimization, we developed a predictive method for designing synthetic ribosome binding sites, enabling a rational control over the protein expression level. Experimental validation of100 predictions in Escherichia coli showed that the method is accurate to within a factor of 2.3 over a range of 100,000-fold. The design method also correctly predicted that reusing identical ribosome binding site sequences in different genetic contexts can result in different protein expression levels. We demonstrate the method's utility by rationally optimizing protein expression to connect a genetic sensor to a synthetic circuit. The proposed forward engineering approach should accelerate the construction and systematic optimization of large genetic systems.

Published
2009

6. Functional optimization of gene clusters by combinatorial design and assembly

Author

Smanski, Michael J, primary, Bhatia, Swapnil, additional, Zhao, Dehua, additional, Park, YongJin, additional, B A Woodruff, Lauren, additional, Giannoukos, Georgia, additional, Ciulla, Dawn, additional, Busby, Michele, additional, Calderon, Johnathan, additional, Nicol, Robert, additional, Gordon, D Benjamin, additional, Densmore, Douglas, additional, and Voigt, Christopher A, additional

Published
2014
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