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Biochemical, structural and cellular studies reveal Jumonji-C (JmjC) domain-containing 7 (JMJD7) to be a 2-oxoglutarate (2OG)-dependent oxygenase that catalyzes (3S)-lysyl hydroxylation. Crystallographic analyses reveal JMJD7 to be more closely related to the JmjC hydroxylases than to the JmjC demethylases. Biophysical and mutation studies show that JMJD7 has a unique dimerization mode, with interactions between monomers involving both N- and C-terminal regions and disulfide bond formation. A proteomic approach identifies two related members of the translation factor (TRAFAC) family of GTPases, developmentally regulated GTP-binding proteins 1 and 2 (DRG1/2), as activity-dependent JMJD7 interactors. Mass spectrometric analyses demonstrate that JMJD7 catalyzes Fe(ii)- and 2OG-dependent hydroxylation of a highly conserved lysine residue in DRG1/2; amino-acid analyses reveal that JMJD7 catalyzes (3S)-lysyl hydroxylation. The functional assignment of JMJD7 will enable future studies to define the role of DRG hydroxylation in cell growth and disease. Fil: Markolovic, Suzana. University of Oxford; Reino Unido Fil: Zhuang, Qinqin. University Of Birmingham; Reino Unido Fil: Wilkins, Sarah E.. University of Oxford; Reino Unido Fil: Eaton, Charlotte D.. University Of Birmingham; Reino Unido Fil: Abboud, Martine I.. University of Oxford; Reino Unido Fil: Katz, Maximiliano Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina Fil: McNeil, Helen E.. University Of Birmingham; Reino Unido Fil: Leśniak, Robert K.. University of Oxford; Reino Unido Fil: Hall, Charlotte. University Of Birmingham; Reino Unido Fil: Struwe, Weston B.. University of Oxford; Reino Unido Fil: Konietzny, Rebecca. University of Oxford; Reino Unido Fil: Davis, Simon. University of Oxford; Reino Unido Fil: Yang, Ming. The Francis Crick Institute; Reino Unido. University of Oxford; Reino Unido Fil: Ge, Wei. University of Oxford; Reino Unido Fil: Benesch, Justin L. P.. University of Oxford; Reino Unido Fil: Kessler, Benedikt M.. University of Oxford; Reino Unido Fil: Ratcliffe, Peter J.. University of Oxford; Reino Unido. The Francis Crick Institute; Reino Unido Fil: Cockman, Matthew E.. The Francis Crick Institute; Reino Unido. University of Oxford; Reino Unido Fil: Fischer, Roman. University of Oxford; Reino Unido Fil: Wappner, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina Fil: Chowdhury, Rasheduzzaman. University of Stanford; Estados Unidos. University of Oxford; Reino Unido Fil: Coleman, Mathew L.. University Of Birmingham; Reino Unido Fil: Schofield, Christopher J.. University of Oxford; Reino Unido

In the version of this article initially published, authors Sarah E. Wilkins, Charlotte D. Eaton, Martine I. Abboud and Maximiliano J. Katz were incorrectly included in the equal contributions footnote in the affiliations list. Footnote number seven linking to the equal contributions statement should be present only for Suzana Markolovic and Qinqin Zhuang, and the statement should read “These authors contributed equally: Suzana Markolovic, Qinqin Zhuang.” The error has been corrected in the HTML and PDF versions of the article.