735 results
Search Results
2. Author Correction: Requirements for the differentiation of innate T-bethigh memory-phenotype CD4+ T lymphocytes under steady state.
- Author
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Kawabe, Takeshi, Yi, Jaeu, Kawajiri, Akihisa, Hilligan, Kerry, Fang, Difeng, Ishii, Naoto, Yamane, Hidehiro, Zhu, Jinfang, Jankovic, Dragana, Kim, Kwang Soon, Trinchieri, Giorgio, and Sher, Alan
- Subjects
T cells ,TECHNICAL specifications - Abstract
An amendment to this paper has been published and can be accessed via a link at the top of the paper. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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3. Publisher Correction: Metabolic characteristics of CD8+ T cell subsets in young and aged individuals are not predictive of functionality.
- Author
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Quinn, Kylie M., Hussain, Tabinda, Kraus, Felix, Formosa, Luke E., Lam, Wai K., Dagley, Michael J., Saunders, Eleanor C., Assmus, Lisa M., Wynne-Jones, Erica, Loh, Liyen, van de Sandt, Carolien E., Cooper, Lucy, Good-Jacobson, Kim L., Kedzierska, Katherine, Mackay, Laura K., McConville, Malcolm J., Ramm, Georg, Ryan, Michael T., and La Gruta, Nicole L.
- Subjects
T cells ,OLDER people - Abstract
An amendment to this paper has been published and can be accessed via a link at the top of the paper. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
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4. Author Correction: Mutational and putative neoantigen load predict clinical benefit of adoptive T cell therapy in melanoma.
- Author
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Lauss, Martin, Donia, Marco, Harbst, Katja, Andersen, Rikke, Mitra, Shamik, Rosengren, Frida, Salim, Maryem, Vallon-Christersson, Johan, Törngren, Therese, Kvist, Anders, Ringnér, Markus, Svane, Inge Marie, and Jönsson, Göran
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CELLULAR therapy ,T cells ,MELANOMA - Abstract
An amendment to this paper has been published and can be accessed via a link at the top of the paper. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
5. Author Correction: In vivo clonal expansion and phenotypes of hypocretin-specific CD4+ T cells in narcolepsy patients and controls.
- Author
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Jiang, Wei, Birtley, James R., Hung, Shu-Chen, Wang, Weiqi, Chiou, Shin-Heng, Macaubas, Claudia, Kornum, Birgitte, Tian, Lu, Huang, Huang, Adler, Lital, Weaver, Grant, Lu, Liying, Ilstad-Minnihan, Alexandra, Somasundaram, Sriram, Ayyangar, Sashi, Davis, Mark M., Stern, Lawrence J., and Mellins, Elizabeth D.
- Subjects
T cells ,NARCOLEPSY ,OREXINS ,PHENOTYPES - Abstract
An amendment to this paper has been published and can be accessed via a link at the top of the paper. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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6. Quantifiable blood TCR repertoire components associate with immune aging.
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Hu, Jing, Pan, Mingyao, Reid, Brett, Tworoger, Shelley, and Li, Bo
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T-cell exhaustion ,OLDER people ,BONE marrow transplantation ,CELL populations ,T cells ,BONE marrow - Abstract
T cell senescence alters the homeostasis of distinct T cell populations and results in decayed adaptive immune protection in older individuals, but a link between aging and dynamic T cell clone changes has not been made. Here, using a newly developed computational framework, Repertoire Functional Units (RFU), we investigate over 6500 publicly available TCR repertoire sequencing samples from multiple human cohorts and identify age-associated RFUs consistently across different cohorts. Quantification of RFU reduction with aging reveals accelerated loss under immunosuppressive conditions. Systematic analysis of age-associated RFUs in clinical samples manifests a potential link between these RFUs and improved clinical outcomes, such as lower ICU admission and reduced risk of complications, during acute viral infections. Finally, patients receiving bone marrow transplantation show a secondary expansion of the age-associated clones upon stem cell transfer from younger donors. Together, our results suggest the existence of a 'TCR clock' that could reflect the immune functions in aging populations. Immune aging is associated with altered homeostasis of distinct T cell populations, but a link to clonal dynamic is still not made. Here the authors develop a new framework, Repertoire Functional Units (RFU), and use public TCR sequences to find specific TCR clonal changes that correlate with aged immune repertoires or outcomes of acute viral infection. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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7. Immune profiling-based targeting of pathogenic T cells with ustekinumab in ANCA-associated glomerulonephritis.
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Engesser, Jonas, Khatri, Robin, Schaub, Darius P., Zhao, Yu, Paust, Hans-Joachim, Sultana, Zeba, Asada, Nariaki, Riedel, Jan-Hendrik, Sivayoganathan, Varshi, Peters, Anett, Kaffke, Anna, Jauch-Speer, Saskia-Larissa, Goldbeck-Strieder, Thiago, Puelles, Victor G., Wenzel, Ulrich O., Steinmetz, Oliver M., Hoxha, Elion, Turner, Jan-Eric, Mittrücker, Hans-Willi, and Wiech, Thorsten
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ANTINEUTROPHIL cytoplasmic antibodies ,KIDNEY failure ,KIDNEY physiology ,DISEASE relapse ,IMMUNOSUPPRESSIVE agents ,T cells - Abstract
Antineutrophil cytoplasmic antibody (ANCA)–associated vasculitis is a life-threatening autoimmune disease that often results in kidney failure caused by crescentic glomerulonephritis (GN). To date, treatment of most patients with ANCA-GN relies on non-specific immunosuppressive agents, which may have serious adverse effects and be only partially effective. Here, using spatial and single-cell transcriptome analysis, we characterize inflammatory niches in kidney samples from 34 patients with ANCA-GN and identify proinflammatory, cytokine-producing CD4
+ and CD8+ T cells as a pathogenic signature. We then utilize these transcriptomic profiles for digital pharmacology and identify ustekinumab, a monoclonal antibody targeting IL-12 and IL-23, as the strongest therapeutic drug to use. Moreover, four patients with relapsing ANCA-GN are treated with ustekinumab in combination with low-dose cyclophosphamide and steroids, with ustekinumab given subcutaneously (90 mg) at weeks 0, 4, 12, and 24. Patients are followed up for 26 weeks to find this treatment well-tolerated and inducing clinical responses, including improved kidney function and Birmingham Vasculitis Activity Score, in all ANCA-GN patients. Our findings thus suggest that targeting of pathogenic T cells in ANCA-GN patients with ustekinumab might represent a potential approach and warrants further investigation in clinical trials. Antineutrophil cytoplasmic antibody (ANCA) is currently treated with broad-spectrum immune suppressive drugs. Here the authors decipher inflammatory niches in the kidney of patients with ANCA-GN by combining spatial and single-cell transcriptomics to identify ustekinumab as a promising treatment option and successfully treat four ANCA-GN patients. [ABSTRACT FROM AUTHOR]- Published
- 2024
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8. Tuning the fluidity and protein corona of ultrasound-responsive liposomal nanovaccines to program T cell immunity in mice.
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He, Jia, Wang, Chaoyu, Fang, Xiao, Li, Junyao, Shen, Xueying, Zhang, Junxia, Peng, Cheng, Li, Hongjian, Li, Sai, Karp, Jeffrey M., and Kuai, Rui
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CANCER vaccines ,INTRAVENOUS injections ,CERVICAL cancer ,IMMUNE response ,SPLEEN ,T cells - Abstract
Inducing high levels of antigen-specific CD8α
+ T cells in the tumor is beneficial for cancer immunotherapy, but achieving this in a safe and effective manner remains challenging. Here, we have developed a designer liposomal nanovaccine containing a sonosensitizer (LNVS) to efficiently program T cell immunity in mice. Following intravenous injection, LNVS accumulates in the spleen in a protein corona and fluidity-dependent manner, leading to greater frequencies of antigen-specific CD8α+ T cells than soluble vaccines (the mixture of antigens and adjuvants). Meanwhile, some LNVS passively accumulates in the tumor, where it responds to ultrasound (US) to increase the levels of chemokines and adhesion molecules that are beneficial for recruiting CD8α+ T cells to the tumor. LNVS + US induces higher levels of intratumoral antitumor T cells than traditional sonodynamic therapy, regresses established mouse MC38 tumors and orthotopic cervical cancer, and protects cured mice from relapse. Our platform sheds light on the importance of tuning the fluidity and protein corona of naovaccines to program T cell immunity in mice and may inspire new strategies for cancer immunotherapy. Delivery and fluidity of cancer nanoparticle vaccines alters efficiency and immune priming. Here the authors show a sonosensitive nanovaccine which depending on fluidity of the vaccine improves vaccine targeting and subsequent anti-tumour immune responses. [ABSTRACT FROM AUTHOR]- Published
- 2024
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9. HIF-2α-dependent induction of miR-29a restrains TH1 activity during T cell dependent colitis.
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Czopik, Agnieszka K., McNamee, Eóin N., Vaughn, Victoria, Huang, Xiangsheng, Bang, In Hyuk, Clark, Trent, Wang, Yanyu, Ruan, Wei, Nguyen, Tom, Masterson, Joanne C., Tak, Eunyoung, Frank, Sandra, Collins, Colm B., Li, Howard, Rodriguez-Aguayo, Cristian, Lopez-Berestein, Gabriel, Gerich, Mark E., Furuta, Glenn T., Yuan, Xiaoyi, and Sood, Anil K.
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INFLAMMATORY bowel diseases ,HYPOXIA-inducible factors ,CELLULAR control mechanisms ,CELL physiology ,TRANSCRIPTION factors ,T cells - Abstract
Metabolic imbalance leading to inflammatory hypoxia and stabilization of hypoxia-inducible transcription factors (HIFs) is a hallmark of inflammatory bowel diseases. We hypothesize that HIF could be stabilized in CD4
+ T cells during intestinal inflammation and alter the functional responses of T cells via regulation of microRNAs. Our assays reveal markedly increased T cell-intrinsic hypoxia and stabilization of HIF protein during experimental colitis. microRNA screen in primary CD4+ T cells points us towards miR-29a and our subsequent studies identify a selective role for HIF-2α in CD4-cell-intrinsic induction of miR-29a during hypoxia. Mice with T cell-intrinsic HIF-2α deletion display elevated T-bet (target of miR-29a) levels and exacerbated intestinal inflammation. Mice with miR-29a deficiency in T cells show enhanced intestinal inflammation. T cell-intrinsic overexpression of HIF-2α or delivery of miR-29a mimetic dampen TH 1-driven colitis. In this work, we show a previously unrecognized function for hypoxia-dependent induction of miR-29a in attenuating TH 1-mediated inflammation. Inflammatory intestinal lesions are often hypoxic, which results in the stabilization and activation of hypoxia-inducible-factors (HIF). Here authors show that in a mouse model of colitis, HIF-2α is specifically stabilized in CD4+ type 1T helper (TH 1) cells, leading to the upregulation of miR-29a expression and suppression of TH 1 cell function, which pathway is potentially targetable for therapeutic purposes. [ABSTRACT FROM AUTHOR]- Published
- 2024
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10. IL-2 delivery to CD8+ T cells during infection requires MRTF/SRF-dependent gene expression and cytoskeletal dynamics.
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Maurice, Diane, Costello, Patrick, Diring, Jessica, Gualdrini, Francesco, Frederico, Bruno, and Treisman, Richard
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TRANSCRIPTION factors ,T cell differentiation ,T cells ,REGULATOR genes ,GENE expression - Abstract
Paracrine IL-2 signalling drives the CD8 + T cell expansion and differentiation that allow protection against viral infections, but the underlying molecular events are incompletely understood. Here we show that the transcription factor SRF, a master regulator of cytoskeletal gene expression, is required for effective IL-2 signalling during L. monocytogenes infection. Acting cell-autonomously with its actin-regulated cofactors MRTF-A and MRTF-B, SRF is dispensible for initial TCR-mediated CD8
+ T cell proliferation, but is required for sustained IL-2 dependent CD8+ effector T cell expansion, and persistence of memory cells. Following TCR activation, Mrtfab-null CD8+ T cells produce IL-2 normally, but homotypic clustering is impaired both in vitro and in vivo. Expression of cytoskeletal structural and regulatory genes, most notably actins, is defective in Mrtfab-null CD8+ T cells. Activation-induced cell clustering in vitro requires F-actin assembly, and Mrtfab-null cell clusters are small, contain less F-actin, and defective in IL-2 retention. Clustering of Mrtfab-null cells can be partially restored by exogenous actin expression. IL-2 mediated CD8+ T cell proliferation during infection thus depends on the control of cytoskeletal dynamics and actin gene expression by MRTF-SRF signalling. The transcription factor SRF, together with its co-factors MRTF-A and MRTF-B, controls cytoskeletal gene expression. Here authors show that MRTF/SRF inactivation leads to decreased IL-2 mediated CD8 + T cell proliferation during infection, resulting from disrupted homotypic cell clustering and reduced retention of IL-2. [ABSTRACT FROM AUTHOR]- Published
- 2024
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11. Targeting PRMT3 impairs methylation and oligomerization of HSP60 to boost anti-tumor immunity by activating cGAS/STING signaling.
- Author
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Shi, Yunxing, Wu, Zongfeng, Liu, Shaoru, Zuo, Dinglan, Niu, Yi, Qiu, Yuxiong, Qiao, Liang, He, Wei, Qiu, Jiliang, Yuan, Yunfei, Wang, Guocan, and Li, Binkui
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IMMUNE checkpoint proteins ,PROTEIN arginine methyltransferases ,HOMEOSTASIS ,HEPATOCELLULAR carcinoma ,LEAKAGE ,T cells ,MITOCHONDRIAL DNA - Abstract
Immune checkpoint blockade (ICB) has emerged as a promising therapeutic option for hepatocellular carcinoma (HCC), but resistance to ICB occurs and patient responses vary. Here, we uncover protein arginine methyltransferase 3 (PRMT3) as a driver for immunotherapy resistance in HCC. We show that PRMT3 expression is induced by ICB-activated T cells via an interferon-gamma (IFNγ)-STAT1 signaling pathway, and higher PRMT3 expression levels correlate with reduced numbers of tumor-infiltrating CD8
+ T cells and poorer response to ICB. Genetic depletion or pharmacological inhibition of PRMT3 elicits an influx of T cells into tumors and reduces tumor size in HCC mouse models. Mechanistically, PRMT3 methylates HSP60 at R446 to induce HSP60 oligomerization and maintain mitochondrial homeostasis. Targeting PRMT3-dependent HSP60 methylation disrupts mitochondrial integrity and increases mitochondrial DNA (mtDNA) leakage, which results in cGAS/STING-mediated anti-tumor immunity. Lastly, blocking PRMT3 functions synergize with PD-1 blockade in HCC mouse models. Our study thus identifies PRMT3 as a potential biomarker and therapeutic target to overcome immunotherapy resistance in HCC. Resistance to immune checkpoint blockade (ICB) is a challenge in hepatocellular carcinoma therapy. Here, the authors show that targeting PRMT3 markedly improves the anticancer efficacy of ICB via interrupting HSP60 methylation and oligomerization, damaging mitochondrial integrity, and thereby activating cGAS/STING-mediated anti-tumor immunity. [ABSTRACT FROM AUTHOR]- Published
- 2024
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12. Genomic and immune heterogeneity of multiple synchronous lung adenocarcinoma at different developmental stages.
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Zhao, Yue, Gao, Jian, Wang, Jun, Fan, Fanfan, Cheng, Chao, Qian, Danwen, Guo, Ran, Zhang, Yang, Ye, Ting, Augustine, Marcellus, Lin, Yicong, Shang, Jun, Li, Hang, Pan, Yunjian, Huang, Qingyuan, Chen, Haiqing, Han, Han, Gao, Zhendong, Wang, Qiming, and Zhang, Shiyue
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REGULATORY T cells ,LUNG cancer ,T cells ,RNA sequencing ,CONVERGENT evolution - Abstract
Multiple synchronous lung cancers (MSLCs) constitute a unique subtype of lung cancer. To explore the genomic and immune heterogeneity across different pathological stages of MSLCs, we analyse 16 MSLCs from 8 patients using single-cell RNA-seq, single-cell TCR sequencing, and bulk whole-exome sequencing. Our investigation indicates clonally independent tumours with convergent evolution driven by shared driver mutations. However, tumours from the same individual exhibit few shared mutations, indicating independent origins. During the transition from pre-invasive to invasive adenocarcinoma, we observe a shift in T cell phenotypes characterized by increased Treg cells and exhausted CD8
+ T cells, accompanied by diminished cytotoxicity. Additionally, invasive adenocarcinomas exhibit greater neoantigen abundance and a more diverse TCR repertoire, indicating heightened heterogeneity. In summary, despite having a common genetic background and environmental exposure, our study emphasizes the individuality of MSLCs at different stages, highlighting their unique genomic and immune characteristics. Multiple synchronous lung cancers (MSLCs) are a subtype of lung cancer. Here the authors characterise MSLCs using single cell RNA sequencing, single cell TCR sequencing and bulk whole-exome sequencing to investigate the mutations that arise in and are associated with invasive adenocarcinoma development, and immune microenvironment changes in this process. [ABSTRACT FROM AUTHOR]- Published
- 2024
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13. Enhanced tumor response to adoptive T cell therapy with PHD2/3-deficient CD8 T cells.
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Dvorakova, Tereza, Finisguerra, Veronica, Formenti, Matteo, Loriot, Axelle, Boudhan, Loubna, Zhu, Jingjing, and Van den Eynde, Benoit J.
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CELL metabolism ,CELL physiology ,HYPOXIA-inducible factors ,IMMUNOSUPPRESSION ,TUMOR microenvironment ,T cells - Abstract
While adoptive cell therapy has shown success in hematological malignancies, its potential against solid tumors is hindered by an immunosuppressive tumor microenvironment (TME). In recent years, members of the hypoxia-inducible factor (HIF) family have gained recognition as important regulators of T-cell metabolism and function. The role of HIF signalling in activated CD8 T cell function in the context of adoptive cell transfer, however, has not been explored in full depth. Here we utilize CRISPR-Cas9 technology to delete prolyl hydroxylase domain-containing enzymes (PHD) 2 and 3, thereby stabilizing HIF-1 signalling, in CD8 T cells that have already undergone differentiation and activation, modelling the T cell phenotype utilized in clinical settings. We observe a significant boost in T-cell activation and effector functions following PHD2/3 deletion, which is dependent on HIF-1α, and is accompanied by an increased glycolytic flux. This improvement in CD8 T cell performance translates into an enhancement in tumor response to adoptive T cell therapy in mice, across various tumor models, even including those reported to be extremely resistant to immunotherapeutic interventions. These findings hold promise for advancing CD8 T-cell based therapies and overcoming the immune suppression barriers within challenging tumor microenvironments. The hypoxia inducible factor HIF-1α has been shown to regulate T cell metabolism and function. Here authors deleted the prolyl hydroxylase domain-containing enzymes PHD2 and 3, thereby stabilizing HIF-1α, in therapeutic CD8 T cells to achieve better functionality upon adoptive transfer to tumour-bearing mice. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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14. Androgens contribute to sex bias of autoimmunity in mice by T cell-intrinsic regulation of Ptpn22 phosphatase expression.
- Author
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Lee, Jean, Yurkovetskiy, Leonid A., Reiman, Derek, Frommer, Lara, Strong, Zoe, Chang, Anthony, Kahaly, George J., Khan, Aly A., and Chervonsky, Alexander V.
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TYPE 1 diabetes ,T cell receptors ,SYSTEMIC lupus erythematosus ,T cells ,AUTOIMMUNE diseases - Abstract
Autoimmune diseases such as systemic lupus erythematosus (SLE) display a strong female bias. Although sex hormones have been associated with protecting males from autoimmunity, the molecular mechanisms are incompletely understood. Here we report that androgen receptor (AR) expressed in T cells regulates genes involved in T cell activation directly, or indirectly via controlling other transcription factors. T cell-specific deletion of AR in mice leads to T cell activation and enhanced autoimmunity in male mice. Mechanistically, Ptpn22, a phosphatase and negative regulator of T cell receptor signaling, is downregulated in AR-deficient T cells. Moreover, a conserved androgen-response element is found in the regulatory region of Ptpn22 gene, and the mutation of this transcription element in non-obese diabetic mice increases the incidence of spontaneous and inducible diabetes in male mice. Lastly, Ptpn22 deficiency increases the disease severity of male mice in a mouse model of SLE. Our results thus implicate AR-regulated genes such as PTPN22 as potential therapeutic targets for autoimmune diseases. Androgen is a sex hormone that may contribute to sex biases in autoimmunity. Here the authors show that in T cells androgen induces the expression of Ptpn22 phosphatase to negatively regulate T cell activation, thereby contributing to protecting males from major autoimmune diseases such as systemic lupus erythematosus and type 1 diabetes. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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15. Discovery of an unconventional lamprey lymphocyte lineage highlights divergent features in vertebrate adaptive immune system evolution.
- Author
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Huang, Yingyi, Liu, Xiang, Li, Shuo, Li, Chen, Wang, Hong-Yan, Liu, Qun, Chen, Jian-Yang, Zhang, Yingying, Li, Yanan, Zhang, Xianghui, Wang, Qian, Liu, Kaiqiang, Liu, Yu-Yan, Pang, Yue, Liu, Shanshan, Fan, Guangyi, and Shao, Changwei
- Subjects
HEMATOPOIETIC growth factors ,BIOLOGICAL evolution ,LYMPHOCYTE subsets ,T cells ,RNA sequencing - Abstract
Lymphocyte receptors independently evolved in both jawed and jawless vertebrates with similar adaptive immune responses. However, the diversity of functional subtypes and molecular architecture in jawless vertebrate lymphocytes, comparable to jawed species, is not well defined. Here, we profile the gills, intestines, and blood of the lamprey, Lampetra morii, with single-cell RNA sequencing, using a full-length transcriptome as a reference. Our findings reveal higher tissue-specific heterogeneity among T-like cells in contrast to B-like cells. Notably, we identify a unique T-like cell subtype expressing a homolog of the nonlymphoid hematopoietic growth factor receptor, MPL-like (MPL-L). These MPL-L+ T-like cells exhibit features distinct from T cells of jawed vertebrates, particularly in their elevated expression of hematopoietic genes. We further discovered that MPL-L
+ VLRA+ T-like cells are widely present in the typhlosole, gill, liver, kidney, and skin of lamprey and they proliferate in response to both a T cell mitogen and recombinant human thrombopoietin. These findings provide new insights into the adaptive immune response in jawless vertebrates, shedding new light on the evolution of adaptive immunity. Lymphocyte subsets remain inadequately understood in jawless vertebrates, relative to jawed vertebrates. In this study, the authors combine single-cell RNA and whole-transcriptome sequencing to demonstrate that the lamprey, Lampreta morii, harbours specialised T-like cells that are distinct from those found in jawed vertebrates. [ABSTRACT FROM AUTHOR]- Published
- 2024
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16. CAR T-cell-mediated delivery of bispecific innate immune cell engagers for neuroblastoma.
- Author
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Pascual-Pasto, Guillem, McIntyre, Brendan, Hines, Margaret G., Giudice, Anna M., Garcia-Gerique, Laura, Hoffmann, Jennifer, Mishra, Pamela, Matlaga, Stephanie, Lombardi, Simona, Shraim, Rawan, Schürch, Patrick M., Yarmarkovich, Mark, Hofmann, Ted J., Alikarami, Fatemeh, Martinez, Daniel, Tsang, Matthew, Gil-de-Gómez, Luis, Spear, Timothy T., Bernt, Kathrin M., and Wolpaw, Adam J.
- Subjects
CHIMERIC antigen receptors ,ENGINEERS ,CYTOTOXINS ,TREATMENT effectiveness ,NEUROBLASTOMA ,T cells - Abstract
Novel chimeric antigen receptor (CAR) T-cell approaches are needed to improve therapeutic efficacy in solid tumors. High-risk neuroblastoma is an aggressive pediatric solid tumor that expresses cell-surface GPC2 and GD2 with a tumor microenvironment infiltrated by CD16a-expressing innate immune cells. Here we engineer T-cells to express a GPC2-directed CAR and simultaneously secrete a bispecific innate immune cell engager (BiCE) targeting both GD2 and CD16a. In vitro, GPC2.CAR-GD2.BiCE T-cells induce GPC2-dependent cytotoxicity and secrete GD2.BiCE that promotes GD2-dependent activation of antitumor innate immunity. In vivo, GPC2.CAR-GD2.BiCE T-cells locally deliver GD2.BiCE and increase intratumor retention of NK-cells. In mice bearing neuroblastoma patient-derived xenografts and reconstituted with human CD16a-expressing immune cells, GD2.BiCEs enhance GPC2.CAR antitumor efficacy. A CAR.BiCE strategy should be considered for tumor histologies where antigen escape limits CAR efficacy, especially for solid tumors like neuroblastoma that are infiltrated by innate immune cells. GPC2 and GD2 have been described as immunotherapeutic targets in neuroblastoma. Here the authors engineer T cells to simultaneously express a GPC2-directed CAR and a bispecific innate immune cell engager targeting GD2 and CD16a, showing antitumor activity in neuroblastoma preclinical models. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
17. ITGB6 modulates resistance to anti-CD276 therapy in head and neck cancer by promoting PF4+ macrophage infiltration.
- Author
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Zhang, Caihua, Li, Kang, Zhu, Hongzhang, Cheng, Maosheng, Chen, Shuang, Ling, Rongsong, Wang, Cheng, and Chen, Demeng
- Subjects
HEAD & neck cancer ,IMMUNE checkpoint inhibitors ,GENE expression ,SQUAMOUS cell carcinoma ,RNA sequencing ,T cells - Abstract
Enoblituzumab, an immunotherapeutic agent targeting CD276, shows both safety and efficacy in activating T cells and oligodendrocyte-like cells against various cancers. Preclinical studies and mouse models suggest that therapies targeting CD276 may outperform PD1/PD-L1 blockade. However, data from mouse models indicate a significant non-responsive population to anti-CD276 treatment, with the mechanisms of resistance still unclear. In this study, we evaluate the activity of anti-CD276 antibodies in a chemically-induced murine model of head and neck squamous cell carcinoma. Using models of induced and orthotopic carcinogenesis, we identify ITGB6 as a key gene mediating differential responses to anti-CD276 treatment. Through single-cell RNA sequencing and gene-knockout mouse models, we find that ITGB6 regulates the expression of the tumor-associated chemokine CX3CL1, which recruits and activates PF4
+ macrophages that express high levels of CX3CR1. Inhibition of the CX3CL1-CX3CR1 axis suppresses the infiltration and secretion of CXCL16 by PF4+ macrophages, thereby reinvigorating cytotoxic CXCR6+ CD8+ T cells and enhancing sensitivity to anti-CD276 treatment. Further investigations demonstrate that inhibiting ITGB6 restores sensitivity to PD1 antibodies in mice resistant to anti-PD1 treatment. In summary, our research reveals a resistance mechanism associated with immune checkpoint inhibitor therapy and identifies potential targets to overcome resistance in cancer treatment. Response rate to anti-CD276 based immunotherapy remains suboptimal in patients with cancer. Here, in a chemically-induced murine model of head and neck squamous cell carcinoma, the authors show that expression of ITGB6 in tumor cells promotes resistance to anti-CD276 therapy, associated with accumulation of PF4+ macrophages and T cell dysfunction. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
18. Targeting ERK-MYD88 interaction leads to ERK dysregulation and immunogenic cancer cell death.
- Author
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Virard, François, Giraud, Stéphane, Bonnet, Mélanie, Magadoux, Léa, Martin, Laetitia, Pham, Thuy Ha, Skafi, Najwa, Deneuve, Sophie, Frem, Rita, Villoutreix, Bruno O., Sleiman, Nawal Hajj, Reboulet, Jonathan, Merabet, Samir, Chaptal, Vincent, Chaveroux, Cédric, Hussein, Nader, Aznar, Nicolas, Fenouil, Tanguy, Treilleux, Isabelle, and Saintigny, Pierre
- Subjects
CELL transformation ,MITOGEN-activated protein kinases ,ADAPTOR proteins ,CANCER cells ,CELL death ,T cells - Abstract
The quest for targeted therapies is critical in the battle against cancer. The RAS/MAP kinase pathway is frequently implicated in neoplasia, with ERK playing a crucial role as the most distal kinase in the RAS signaling cascade. Our previous research demonstrated that the interaction between ERK and MYD88, an adaptor protein in innate immunity, is crucial for RAS-dependent transformation and cancer cell survival. In this study, we examine the biological consequences of disrupting the ERK-MYD88 interaction through the ERK D-recruitment site (DRS), while preserving ERK's kinase activity. Our results indicate that EI-52, a small-molecule benzimidazole targeting ERK-MYD88 interaction induces an HRI-mediated integrated stress response (ISR), resulting in immunogenic apoptosis specific to cancer cells. Additionally, EI-52 exhibits anti-tumor efficacy in patient-derived tumors and induces an anti-tumor T cell response in mice in vivo. These findings suggest that inhibiting the ERK-MYD88 interaction may be a promising therapeutic approach in cancer treatment. The interaction between MyD88 and ERK is necessary for RAS-dependent transformation and cancer cell survival. Here, the authors identify benzimidazole compound EI-52 as a disrupter of this interaction and demonstrate its therapeutic efficacy in tumours via ERK dysregulation and the induction of immunogenic cell death. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
19. Inhibition of glycosphingolipid synthesis with eliglustat in combination with immune checkpoint inhibitors in advanced cancers: preclinical evidence and phase I clinical trial.
- Author
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Dong, Liang, Cao, Zhi, Chen, Meixia, Liu, Yang, Ma, Xinran, Lu, Yuting, Zhang, Yan, Feng, Kaichao, Zhang, Yang, Meng, Zhenzhen, Yang, Qingming, Wang, Yao, Wu, Zhiqiang, and Han, Weidong
- Subjects
IMMUNE checkpoint inhibitors ,MAJOR histocompatibility complex ,CANCER cells ,IMMUNE response ,GLYCOSPHINGOLIPIDS ,T cell receptors ,T cells - Abstract
Glycosphingolipids (GSLs) are abundantly expressed in cancer cells. The effects of GSL-targeted immunotherapies are not fully understood. Here, we show that the inhibition of GSL synthesis with the UDP-glucose ceramide glucosyltransferase inhibitor eliglustat can increase the exposure of the major histocompatibility complex (MHC) and tumour antigen peptides, enhancing the antitumour response of CD8
+ T cells in a range of tumour models. We therefore conducted a proof-of-concept phase I trial on the combination of eliglustat and an anti-PD-1 antibody for the treatment of advanced cancers (NCT04944888). The primary endpoints were safety and feasibility, and the secondary endpoint was antitumor activity. All prespecified endpoints were met. Among the 31 enrolled patients, only 1 patient experienced a grade 3 adverse event (AE), and no grade 4 AEs were observed. The objective response rate was 22.6% and the disease control rate reached 71%. Of the 8 patients with proficient mismatch repair/microsatellite stable (pMMR/MSS) colorectal cancer, one achieved complete response and two each had partial response and stable disease. In summary, inhibiting the synthesis of GSLs might represent an effective immunotherapy approach. Glycosphingolipids (GSLs) are abnormally expressed in cancer cells. Here, in addition to showing that inhibition of the synthesis of GSLs with eliglustat promotes anti-tumor immune responses in preclinical models, the authors report the results of a phase I trial of eliglustat and anti-PD-1 antibodies for the treatment of advanced cancers. [ABSTRACT FROM AUTHOR]- Published
- 2024
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- View/download PDF
20. TFEB activation hallmarks antigenic experience of B lymphocytes and directs germinal center fate decisions.
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Münchhalfen, Matthias, Görg, Richard, Haberl, Michael, Löber, Jens, Willenbrink, Jakob, Schwarzt, Laura, Höltermann, Charlotte, Ickes, Christian, Hammermann, Leonard, Kus, Jan, Chapuy, Björn, Ballabio, Andrea, Reichardt, Sybille D., Flügel, Alexander, Engels, Niklas, and Wienands, Jürgen
- Subjects
TRANSCRIPTION factors ,B cells ,IMMUNOLOGIC memory ,T cells ,GERMINAL centers ,B cell receptors ,CHEMOKINE receptors - Abstract
Ligation of the B cell antigen receptor (BCR) initiates humoral immunity. However, BCR signaling without appropriate co-stimulation commits B cells to death rather than to differentiation into immune effector cells. How BCR activation depletes potentially autoreactive B cells while simultaneously primes for receiving rescue and differentiation signals from cognate T lymphocytes remains unknown. Here, we use a mass spectrometry-based proteomic approach to identify cytosolic/nuclear shuttling elements and uncover transcription factor EB (TFEB) as a central BCR-controlled rheostat that drives activation-induced apoptosis, and concurrently promotes the reception of co-stimulatory rescue signals by supporting B cell migration and antigen presentation. CD40 co-stimulation prevents TFEB-driven cell death, while enhancing and prolonging TFEB's nuclear residency, which hallmarks antigenic experience also of memory B cells. In mice, TFEB shapes the transcriptional landscape of germinal center B cells. Within the germinal center, TFEB facilitates the dark zone entry of light-zone-residing centrocytes through regulation of chemokine receptors and, by balancing the expression of Bcl-2/BH3-only family members, integrates antigen-induced apoptosis with T cell-provided CD40 survival signals. Thus, TFEB reprograms antigen-primed germinal center B cells for cell fate decisions. B cell receptor activation leads to contrary outcomes in the absence and presence of co-stimulation. Here, the authors show transcription factor EB acts as a B cell receptor-controlled rheostat that balances activation-induced cell death with co-stimulatory rescue signals, collectively reprograming antigen-primed germinal center B cells for fate decisions. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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21. Dysfunctional tumor-infiltrating Vδ1 + T lymphocytes in microsatellite-stable colorectal cancer.
- Author
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Stary, Victoria, Pandey, Ram V., List, Julia, Kleissl, Lisa, Deckert, Florian, Kabiljo, Julijan, Laengle, Johannes, Gerakopoulos, Vasileios, Oehler, Rudolf, Watzke, Lukas, Farlik, Matthias, Lukowski, Samuel W., Vogt, Anne B., Stary, Georg, Stockinger, Hannes, Bergmann, Michael, and Pilat, Nina
- Subjects
T cells ,IMMUNOSUPPRESSION ,IMMUNE recognition ,TUMOR-infiltrating immune cells ,COLORECTAL cancer - Abstract
Although γδ T cells are known to participate in immune dysregulation in solid tumors, their relevance to human microsatellite-stable (MSS) colorectal cancer (CRC) is still undefined. Here, using integrated gene expression analysis and T cell receptor sequencing, we characterized γδ T cells in MSS CRC, with a focus on Vδ1 + T cells. We identified Vδ1
+ T cells with shared motifs in the third complementarity-determining region of the δ-chain, reflective of antigen recognition. Changes in gene and protein expression levels suggested a dysfunctional effector state of Vδ1+ T cells in MSS CRC, distinct from Vδ1+ T cells in microsatellite-instable (MSI). Interaction analysis highlighted an immunosuppressive role of fibroblasts in the dysregulation of Vδ1+ T cells in MSS CRC via the TIGIT-NECTIN2 axis. Blocking this pathway with a TIGIT antibody partially restored cytotoxicity of the dysfunctional Vδ1 phenotype. These results define an operative pathway in γδ T cells in MSS CRC. Although γδ T cells are known to participate in immune dysregulation in solid tumors, their relevance to human microsatellite-stable (MSS) colorectal cancer (CRC) is less well-studied. Here, using single-cell RNA-sequencing, the authors identify a Vδ1 + T cell subset, which are functionally impaired in MSS CRC via a TIGIT-NECTIN2 interaction. [ABSTRACT FROM AUTHOR]- Published
- 2024
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22. Single-cell profiling identifies a CD8bright CD244bright Natural Killer cell subset that reflects disease activity in HLA-A29-positive birdshot chorioretinopathy.
- Author
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Nath, Pulak R., Maclean, Mary, Nagarajan, Vijay, Lee, Jung Wha, Yakin, Mehmet, Kumar, Aman, Nadali, Hadi, Schmidt, Brian, Kaya, Koray D., Kodati, Shilpa, Young, Alice, Caspi, Rachel R., Kuiper, Jonas J. W., and Sen, H. Nida
- Subjects
KILLER cells ,T cells ,CELL receptors ,CELLULAR recognition ,CELL populations - Abstract
Birdshot chorioretinopathy is an inflammatory eye condition strongly associated with MHC-I allele HLA-A29. The striking association with MHC-I suggests involvement of T cells, whereas natural killer (NK) cell involvement remains largely unstudied. Here we show that HLA-A29-positive birdshot chorioretinopathy patients have a skewed NK cell pool containing expanded CD16 positive NK cells which produce more proinflammatory cytokines. These NK cells contain populations that express CD8A which is involved in MHC-I recognition on target cells, display gene signatures indicative of high cytotoxic activity (GZMB, PRF1 and ISG15), and signaling through NK cell receptor CD244 (SH2D1B). Long-term monitoring of a cohort of birdshot chorioretinopathy patients with active disease identifies a population of CD8
bright CD244bright NK cells, which rapidly declines to normal levels upon clinical remission following successful treatment. Collectively, these studies implicate CD8bright CD244bright NK cells in birdshot chorioretinopathy. Birdshot chorioretinopathy is an inflammatory eye condition strongly associated with HLA-A29. Here the authors use a single cell RNA sequencing approach to characterise NK cell involvement in this disease and show that CD8bright CD244bright NK cells are associated with active disease. [ABSTRACT FROM AUTHOR]- Published
- 2024
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23. Targeting myeloid-derived suppressor cells promotes antiparasitic T-cell immunity and enhances the efficacy of PD-1 blockade (15 words).
- Author
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Zhang, Chuanshan, Wang, Hui, Aji, Tuerganaili, Li, Zhide, Li, Yinshi, Ainiwaer, Abidan, Rousu, Zibigu, Li, Jing, Wang, Maolin, Deng, Bingqing, duolikun, Adilai, Kang, Xuejiao, Zheng, Xuran, Yu, Qian, Shao, Yingmei, Zhang, Wenbao, Vuitton, Dominique A., Tian, Zhigang, Sun, Haoyu, and Wen, Hao
- Subjects
MYELOID-derived suppressor cells ,SUPPRESSOR cells ,T cells ,PROGRAMMED cell death 1 receptors ,ECHINOCOCCUS multilocularis ,IMMUNE response - Abstract
Immune exhaustion corresponds to a loss of effector function of T cells that associates with cancer or chronic infection. Here, our objective was to decipher the mechanisms involved in the immune suppression of myeloid-derived suppressor cells (MDSCs) and to explore the potential to target these cells for immunotherapy to enhance checkpoint blockade efficacy in a chronic parasite infection. We demonstrated that programmed cell-death-1 (PD-1) expression was significantly upregulated and associated with T-cell dysfunction in advanced alveolar echinococcosis (AE) patients and in Echinococcus multilocularis-infected mice. PD-1 blockade ex vivo failed to reverse AE patients' peripheral blood T-cell dysfunction. PD-1/PD-L1 blockade or PD-1 deficiency had no significant effects on metacestode in mouse model. This was due to the inhibitory capacities of immunosuppressive granulocytic MDSCs (G-MDSCs), especially in the liver surrounding the parasite pseudotumor. MDSCs suppressed T-cell function in vitro in an indoleamine 2, 3 dioxygenase 1 (IDO1)-dependent manner. Although depleting MDSCs alone restored T-cell effector functions and led to some limitation of disease progression in E. multilocularis-infected mice, combination with PD-1 blockade was better to induce antiparasitic efficacy. Our findings provide preclinical evidence in support of targeting MDSC or combining such an approach with checkpoint blockade in patients with advanced AE. (200 words) Myeloid-derived suppressor cells play an important role in immune suppression caused by Echinococcus multilocularis infection. This study demonstrates that the removal of this cell type effectively restores T-cell function and potentiates PD-1 blockade therapy in chronic liver infection. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
24. Ca2+ transients on the T cell surface trigger rapid integrin activation in a timescale of seconds.
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Li, Yue, Wang, ShiHui, Zhang, YouHua, Liu, ZhaoYuan, Zheng, YunZhe, Zhang, Kun, Chen, ShiYang, Lv, XiaoYing, Huang, MengWen, Pan, XingChao, Zheng, YaJuan, Yuan, MengYa, Ge, GaoXiang, Zeng, Yi Arial, Lin, ChangDong, and Chen, JianFeng
- Subjects
T cells ,LYMPHOCYTE transformation ,SKIN inflammation ,LYMPHOCYTES ,INTEGRINS ,CHEMOKINE receptors ,LABORATORY mice - Abstract
One question in lymphocyte homing is how integrins are rapidly activated to enable immediate arrest of fast rolling lymphocytes upon encountering chemokines at target vascular beds given the slow chemokine-induced integrin inside-out activation. Herein we demonstrate that chemokine CCL25-triggered Ca
2+ influx induces T cell membrane-proximal external Ca2+ concentration ([Ca2+ ]ex ) drop in 6 s from physiological concentration 1.2 mM to 0.3 mM, a critical extracellular Ca2+ threshold for inducing αLβ2 activation, triggering rapid αLβ2 activation and T cell arrest before occurrence of αLβ2 inside-out activation. Talin knockdown inhibits the slow inside-out activation of αLβ2 but not [Ca2+ ]ex drop-triggered αLβ2 quick activation. Blocking Ca2+ influx significantly suppresses T cell rolling-to-arrest transition and homing to skin lesions in a mouse psoriasis model, thus alleviating skin inflammation. [Ca2+ ]ex decrease-triggered rapid integrin activation bridges the gap between initial chemokine stimulation and slow integrin inside-out activation, ensuring immediate lymphocyte arrest and subsequent diapedesis on the right location. Lymphocytes need to be slowed down rapidly to enter tissues. Here the authors characterise the arrest of lymphocytes and using a calcium biosensor propose that a rapid drop in extracellular calcium concentration results in integrin activation and lymphocyte arrest. [ABSTRACT FROM AUTHOR]- Published
- 2024
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25. Type II innate lymphoid cell plasticity contributes to impaired reconstitution after allogeneic hematopoietic stem cell transplantation.
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Laurie, Sonia J., Foster II, Joseph P., Bruce, Danny W., Bommiasamy, Hemamalini, Kolupaev, Oleg V., Yazdimamaghani, Mostafa, Pattenden, Samantha G., Chao, Nelson J., Sarantopoulos, Stefanie, Parker, Joel S., Davis, Ian J., and Serody, Jonathan S.
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HEMATOPOIETIC stem cell transplantation ,INNATE lymphoid cells ,T cells ,HEMATOPOIETIC stem cells ,MUCOUS membranes ,GRAFT versus host disease ,SMALL intestine ,HOMEOSTASIS ,IMMUNOPRECIPITATION - Abstract
Type II innate lymphoid cells (ILC2s) maintain homeostasis and barrier integrity in mucosal tissues. In both mice and humans, ILC2s poorly reconstitute after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Determining the mechanisms involved in their impaired reconstitution could improve transplant outcomes. By integrating single-cell chromatin and transcriptomic analyses of transplanted ILC2s, we identify a previously unreported population of converted ILC1-like cells in the mouse small intestine post-transplant. Exposure of ILC2s to proinflammatory cytokines resulted in a mixed ILC1-ILC2 phenotype but was able to convert only a small population of ILC2s to ILC1s, which were found post-transplant. Whereas ILC2s protected against acute graft-versus-host disease (aGVHD) mediated mortality, infusion of proinflammatory cytokine-exposed ILC2s accelerated aGvHD. Interestingly, murine ILC2 reconstitution post-HSCT is decreased in the presence of alloreactive T cells. Finally, peripheral blood cells from human patients with aGvHD have an altered ILC2-associated chromatin landscape compared to transplanted controls. These data demonstrate that following transplantation ILC2s convert to a pro-pathogenic population with an ILC1-like chromatin state and provide insights into the contribution of ILC plasticity to the impaired reconstitution of ILC2 cells, which is one of several potential mechanisms for the poor reconstitution of these important cells after allo-HSCT. Allogeneic hematopoietic stem cell transplantation restores the whole hematopoietic compartment of the recipient, but some cell types, such as Type II innate lymphoid cells (ILC2) are not reconstituted efficiently. Here authors show that ILC2s could be converted to ILC1-like cells in the mouse small intestine post-transplantation, which might contribute to the lower than physiological ILC2 numbers. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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26. Terminal deoxynucleotidyl transferase and CD84 identify human multi-potent lymphoid progenitors.
- Author
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Kim, YeEun, Calderon, Ariel A., Favaro, Patricia, Glass, David R., Tsai, Albert G., Ho, Daniel, Borges, Luciene, Greenleaf, William J., and Bendall, Sean C.
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TRANSCRIPTION factors ,BONE marrow ,TRANSFERASES ,T cells ,HEMATOPOIESIS ,HUMAN beings ,DNA polymerases - Abstract
Lymphoid specification in human hematopoietic progenitors is not fully understood. To better associate lymphoid identity with protein-level cell features, we conduct a highly multiplexed single-cell proteomic screen on human bone marrow progenitors. This screen identifies terminal deoxynucleotidyl transferase (TdT), a specialized DNA polymerase intrinsic to VDJ recombination, broadly expressed within CD34
+ progenitors prior to B/T cell emergence. While these TdT+ cells coincide with granulocyte-monocyte progenitor (GMP) immunophenotype, their accessible chromatin regions show enrichment for lymphoid-associated transcription factor (TF) motifs. TdT expression on GMPs is inversely related to the SLAM family member CD84. Prospective isolation of CD84lo GMPs demonstrates robust lymphoid potentials ex vivo, while still retaining significant myeloid differentiation capacity, akin to LMPPs. This multi-omic study identifies human bone marrow lymphoid-primed progenitors, further defining the lympho-myeloid axis in human hematopoiesis. How lymphoid and myeloid specification occurs in human haematopoietic progenitors is not fully understood. Here the authors perform a proteomic screen on human bone marrow progenitors and suggest TdT+ and CD84- progenitors as lymphoid-primed progenitors with residual myeloid potentials. [ABSTRACT FROM AUTHOR]- Published
- 2024
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27. Adeno-associated virus delivered CXCL9 sensitizes glioblastoma to anti-PD-1 immune checkpoint blockade.
- Author
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von Roemeling, Christina A., Patel, Jeet A., Carpenter, Savannah L., Yegorov, Oleg, Yang, Changlin, Bhatia, Alisha, Doonan, Bently P., Russell, Rylynn, Trivedi, Vrunda S., Klippel, Kelena, Ryu, Daniel H., Grippin, Adam, Futch, Hunter S., Ran, Yong, Hoang-Minh, Lan B., Weidert, Frances L., Golde, Todd E., and Mitchell, Duane A.
- Subjects
IMMUNE checkpoint proteins ,ADENO-associated virus ,GLIOBLASTOMA multiforme ,TREATMENT effectiveness ,LYMPHOKINES ,T cells ,CHEMOKINE receptors - Abstract
There are numerous mechanisms by which glioblastoma cells evade immunological detection, underscoring the need for strategic combinatorial treatments to achieve appreciable therapeutic effects. However, developing combination therapies is difficult due to dose-limiting toxicities, blood-brain-barrier, and suppressive tumor microenvironment. Glioblastoma is notoriously devoid of lymphocytes driven in part by a paucity of lymphocyte trafficking factors necessary to prompt their recruitment and activation. Herein, we develop a recombinant adeno-associated virus (AAV) gene therapy that enables focal and stable reconstitution of the tumor microenvironment with C-X-C motif ligand 9 (CXCL9), a powerful call-and-receive chemokine for lymphocytes. By manipulating local chemokine directional guidance, AAV-CXCL9 increases tumor infiltration by cytotoxic lymphocytes, sensitizing glioblastoma to anti-PD-1 immune checkpoint blockade in female preclinical tumor models. These effects are accompanied by immunologic signatures evocative of an inflamed tumor microenvironment. These findings support AAV gene therapy as an adjuvant for reconditioning glioblastoma immunogenicity given its safety profile, tropism, modularity, and off-the-shelf capability. The limited infiltration and migration of T cells in the brain can hinder the success of immune checkpoint blockade in glioblastoma (GBM). Here the authors show that an adeno-associated virus-based gene therapy for the intratumor delivery of CXCL9 promotes T cell infiltration and enhances response to anti-PD1 in GBM preclinical models. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
28. Heterogeneity in lung macrophage control of Mycobacterium tuberculosis is modulated by T cells.
- Author
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Lai, Rocky, Williams, Travis, Rakib, Tasfia, Lee, Jinhee, and Behar, Samuel M.
- Subjects
MYCOBACTERIUM tuberculosis ,T cells ,CELLULAR recognition ,ALVEOLAR macrophages ,LUNGS ,MACROPHAGES ,T cell receptors ,IMMUNOSENESCENCE - Abstract
Following Mycobacterium tuberculosis infection, alveolar macrophages are initially infected but ineffectively restrict bacterial replication. The distribution of M. tuberculosis among different cell types in the lung changes with the onset of T cell immunity when the dominant infected cellular niche shifts from alveolar to monocyte-derived macrophages (MDM). We hypothesize that changes in bacterial distribution among different cell types is driven by differences in T cell recognition of infected cells and their subsequent activation of antimicrobial effector mechanisms. We show that CD4 and CD8 T cells efficiently eliminate M. tuberculosis infection in alveolar macrophages, but they have less impact on suppressing infection in MDM, which may be a bacterial niche. Importantly, CD4 T cell responses enhance MDM recruitment to the lung. Thus, the outcome of infection depends on the interaction between the T cell subset and the infected cell; both contribute to the resolution and persistence of the infection. Both T cells and lung macrophages are involved in the control of lung tuberculosis infection. Here the authors examine the dependence of the lung macrophage response upon the presence of T cells and show that CD4 and CD8 T cells promote the elimination of M.Tb in alveolar macrophages but have less impact on monocyte-derived macrophages. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
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29. Using a pan-cancer atlas to investigate tumour associated macrophages as regulators of immunotherapy response.
- Author
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Coulton, Alexander, Murai, Jun, Qian, Danwen, Thakkar, Krupa, Lewis, Claire E., and Litchfield, Kevin
- Subjects
MACROPHAGES ,T cells ,IMMUNE checkpoint proteins ,REFERENCE sources ,TUMORS - Abstract
The paradigm for macrophage characterization has evolved from the simple M1/M2 dichotomy to a more complex model that encompasses the broad spectrum of macrophage phenotypic diversity, due to differences in ontogeny and/or local stimuli. We currently lack an in-depth pan-cancer single cell RNA-seq (scRNAseq) atlas of tumour-associated macrophages (TAMs) that fully captures this complexity. In addition, an increased understanding of macrophage diversity could help to explain the variable responses of cancer patients to immunotherapy. Our atlas includes well established macrophage subsets as well as a number of additional ones. We associate macrophage composition with tumour phenotype and show macrophage subsets can vary between primary and metastatic tumours growing in sites like the liver. We also examine macrophage-T cell functional cross talk and identify two subsets of TAMs associated with T cell activation. Analysis of TAM signatures in a large cohort of immune checkpoint inhibitor-treated patients (CPI1000 +) identify multiple TAM subsets associated with response, including the presence of a subset of TAMs that upregulate collagen-related genes. Finally, we demonstrate the utility of our data as a resource and reference atlas for mapping of novel macrophage datasets using projection. Overall, these advances represent an important step in both macrophage classification and overcoming resistance to immunotherapies in cancer. Single cell sequencing can be used to examine tumour associated macrophages (TAM) and comparison between studies has been a challenge. Here the authors show a comparison tool to compare and contrast TAMs from different human tumour types and how these cells associate with T cells exploring further macrophage heterogeneity. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
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30. Three SARS-CoV-2 spike protein variants delivered intranasally by measles and mumps vaccines are broadly protective.
- Author
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Zhang, Yuexiu, Chamblee, Michelle, Xu, Jiayu, Qu, Panke, Shamseldin, Mohamed M., Yoo, Sung J., Misny, Jack, Thongpan, Ilada, KC, Mahesh, Hall, Jesse M., Gupta, Yash A., Evans, John P., Lu, Mijia, Ye, Chengjin, Hsu, Cheng Chih, Liang, Xueya, Martinez-Sobrido, Luis, Yount, Jacob S., Boyaka, Prosper N., and Liu, Shan-Lu
- Subjects
SARS-CoV-2 Omicron variant ,MEASLES vaccines ,COMBINED vaccines ,COVID-19 vaccines ,SARS-CoV-2 ,T cells - Abstract
As the new SARS-CoV-2 Omicron variants and subvariants emerge, there is an urgency to develop intranasal, broadly protective vaccines. Here, we developed highly efficacious, intranasal trivalent SARS-CoV-2 vaccine candidates (TVC) based on three components of the MMR vaccine: measles virus (MeV), mumps virus (MuV) Jeryl Lynn (JL1) strain, and MuV JL2 strain. Specifically, MeV, MuV-JL1, and MuV-JL2 vaccine strains, each expressing prefusion spike (preS-6P) from a different variant of concern (VoC), were combined to generate TVCs. Intranasal immunization of IFNAR1
−/− mice and female hamsters with TVCs generated high levels of S-specific serum IgG antibodies, broad neutralizing antibodies, and mucosal IgA antibodies as well as tissue-resident memory T cells in the lungs. The immunized female hamsters were protected from challenge with SARS-CoV-2 original WA1, B.1.617.2, and B.1.1.529 strains. The preexisting MeV and MuV immunity does not significantly interfere with the efficacy of TVC. Thus, the trivalent platform is a promising next-generation SARS-CoV-2 vaccine candidate. In this study, the authors developed intranasal measles virus and mumps virus-based trivalent vaccines, each expressing three distinct SARS-CoV-2 stabilized prefusion spike proteins. They show that the intranasal vaccines provide protection against infection of SARS-CoV-2 variants in small animal models. [ABSTRACT FROM AUTHOR]- Published
- 2024
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31. Multi-modal generative modeling for joint analysis of single-cell T cell receptor and gene expression data.
- Author
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Drost, Felix, An, Yang, Bonafonte-Pardàs, Irene, Dratva, Lisa M., Lindeboom, Rik G. H., Haniffa, Muzlifah, Teichmann, Sarah A., Theis, Fabian, Lotfollahi, Mohammad, and Schubert, Benjamin
- Subjects
GENE expression ,DEEP learning ,CELL physiology ,T cell receptors ,T cells ,KNOWLEDGE transfer - Abstract
Recent advances in single-cell immune profiling have enabled the simultaneous measurement of transcriptome and T cell receptor (TCR) sequences, offering great potential for studying immune responses at the cellular level. However, integrating these diverse modalities across datasets is challenging due to their unique data characteristics and technical variations. Here, to address this, we develop the multimodal generative model mvTCR to fuse modality-specific information across transcriptome and TCR into a shared representation. Our analysis demonstrates the added value of multimodal over unimodal approaches to capture antigen specificity. Notably, we use mvTCR to distinguish T cell subpopulations binding to SARS-CoV-2 antigens from bystander cells. Furthermore, when combined with reference mapping approaches, mvTCR can map newly generated datasets to extensive T cell references, facilitating knowledge transfer. In summary, we envision mvTCR to enable a scalable analysis of multimodal immune profiling data and advance our understanding of immune responses. Although single-cell RNA sequencing analysis now allows simultaneous examination of transcriptome and T cell receptor repertoire sequences, integrating these two modalities remains a challenge. Here, the authors develop mvTCR, a generative deep learning model that integrates transcriptome and T cell receptor data into a joint representation capturing cell functions and phenotypes. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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- View/download PDF
32. Publisher Correction: ER stress-induced mediator C/EBP homologous protein thwarts effector T cell activity in tumors through T-bet repression.
- Author
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Cao, Yu, Trillo-Tinoco, Jimena, Sierra, Rosa A., Anadon, Carmen, Dai, Wenjie, Mohamed, Eslam, Cen, Ling, Costich, Tara L., Magliocco, Anthony, Marchion, Douglas, Klar, Richard, Michel, Sven, Jaschinski, Frank, Reich, Richard R., Mehrotra, Shikhar, Cubillos-Ruiz, Juan R., Munn, David H., Conejo-Garcia, Jose R., and Rodriguez, Paulo C.
- Subjects
ENDOPLASMIC reticulum ,T cells ,TUMORS - Abstract
An amendment to this paper has been published and can be accessed via a link at the top of the paper. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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33. Author Correction: Human FCHO1 deficiency reveals role for clathrin-mediated endocytosis in development and function of T cells.
- Author
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Łyszkiewicz, Marcin, Ziętara, Natalia, Frey, Laura, Pannicke, Ulrich, Stern, Marcel, Liu, Yanshan, Fan, Yanxin, Puchałka, Jacek, Hollizeck, Sebastian, Somekh, Ido, Rohlfs, Meino, Yilmaz, Tuğba, Ünal, Ekrem, Karakukcu, Musa, Patiroğlu, Türkan, Kellerer, Christina, Karasu, Ebru, Sykora, Karl-Walter, Lev, Atar, and Simon, Amos
- Subjects
T cells ,ENDOCYTOSIS ,CELL physiology - Abstract
An amendment to this paper has been published and can be accessed via a link at the top of the paper. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
34. HIV-1 Vpr combats the PU.1-driven antiviral response in primary human macrophages.
- Author
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Virgilio, Maria C., Ramnani, Barkha, Chen, Thomas, Disbennett, W. Miguel, Lubow, Jay, Welch, Joshua D., and Collins, Kathleen L.
- Subjects
TRANSCRIPTION factors ,HIV ,MACROPHAGES ,VIRAL transmission ,IMMUNE response ,PROTEOLYSIS ,T cells - Abstract
HIV-1 Vpr promotes efficient spread of HIV-1 from macrophages to T cells by transcriptionally downmodulating restriction factors that target HIV-1 Envelope protein (Env). Here we find that Vpr induces broad transcriptomic changes by targeting PU.1, a transcription factor necessary for expression of host innate immune response genes, including those that target Env. Consistent with this, we find silencing PU.1 in infected macrophages lacking Vpr rescues Env. Vpr downmodulates PU.1 through a proteasomal degradation pathway that depends on physical interactions with PU.1 and DCAF1, a component of the Cul4A E3 ubiquitin ligase. The capacity for Vpr to target PU.1 is highly conserved across primate lentiviruses. In addition to impacting infected cells, we find that Vpr suppresses expression of innate immune response genes in uninfected bystander cells, and that virion-associated Vpr can degrade PU.1. Together, we demonstrate Vpr counteracts PU.1 in macrophages to blunt antiviral immune responses and promote viral spread. Virgilio et al show that HIV Vpr promotes the degradation of the myeloid transcription factor, PU.1, to prevent the expression of PU.1-regulated antiviral factors that would otherwise target HIV Env and inhibit viral spread in macrophages. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
35. CD4+ T cells display a spectrum of recall dynamics during re-infection with malaria parasites.
- Author
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Lee, Hyun Jae, Moreira, Marcela L., Li, Shihan, Asatsuma, Takahiro, Williams, Cameron G., Skinner, Oliver P., Asad, Saba, Bramhall, Michael, Jiang, Zhe, Liu, Zihan, Kerr, Ashlyn S., Engel, Jessica A., Soon, Megan S. F., Straube, Jasmin, Barrera, Irving, Murray, Evan, Chen, Fei, Nideffer, Jason, Jagannathan, Prasanna, and Haque, Ashraful
- Subjects
T cells ,REINFECTION ,PLASMODIUM ,GRAPHICAL user interfaces ,T cell receptors ,TRANSCRIPTOMES - Abstract
Children in malaria-endemic regions can experience repeated Plasmodium infections over short periods of time. Effects of re-infection on multiple co-existing CD4
+ T cell subsets remain unresolved. Here, we examine antigen-experienced CD4+ T cells during re-infection in mice, using scRNA-seq/TCR-seq and spatial transcriptomics. TCR transgenic TEM cells initiate rapid Th1/Tr1 recall responses prior to proliferating, while GC Tfh counterparts are refractory, with TCM /Tfh-like cells exhibiting modest non-proliferative responses. Th1-recall is a partial facsimile of primary Th1-responses, with no upregulated effector-associated genes being unique to recall. Polyclonal, TCR-diverse, CD4+ T cells exhibit similar recall dynamics, with individual clones giving rise to multiple effectors including highly proliferative Th1/Tr1 cells, as well as GC Tfh and Tfh-like cells lacking proliferative capacity. Thus, we show substantial diversity in recall responses mounted by multiple co-existing CD4+ T cell subsets in the spleen, and present graphical user interfaces for studying gene expression dynamics and clonal relationships during re-infection. CD4+ T cells are known to be important in Plasmodium infection. Here the authors use mouse models to track antigen-experienced TCR transgenic and polyclonal CD4+ T cells during Plasmodium re-infection, and show different T cell phenotypes and varied responses in different areas of the spleen. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
36. Mycobacterium tuberculosis strain with deletions in menT3 and menT4 is attenuated and confers protection in mice and guinea pigs.
- Author
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Gosain, Tannu Priya, Chugh, Saurabh, Rizvi, Zaigham Abbas, Chauhan, Neeraj Kumar, Kidwai, Saqib, Thakur, Krishan Gopal, Awasthi, Amit, and Singh, Ramandeep
- Subjects
GUINEA pigs ,MYCOBACTERIUM tuberculosis ,T cells ,MICE ,IMMUNOLOGIC memory ,BACTERIAL toxins ,OXIDATIVE stress - Abstract
The genome of Mycobacterium tuberculosis encodes for a large repertoire of toxin-antitoxin systems. In the present study, MenT3 and MenT4 toxins belonging to MenAT subfamily of TA systems have been functionally characterized. We demonstrate that ectopic expression of these toxins inhibits bacterial growth and this is rescued upon co-expression of their cognate antitoxins. Here, we show that simultaneous deletion of menT3 and menT4 results in enhanced susceptibility of M. tuberculosis upon exposure to oxidative stress and attenuated growth in guinea pigs and mice. We observed reduced expression of transcripts encoding for proteins that are essential or required for intracellular growth in mid-log phase cultures of ΔmenT4ΔT3 compared to parental strain. Further, the transcript levels of proteins involved in efficient bacterial clearance were increased in lung tissues of ΔmenT4ΔT3 infected mice relative to parental strain infected mice. We show that immunization of mice and guinea pigs with ΔmenT4ΔT3 confers significant protection against M. tuberculosis infection. Remarkably, immunization of mice with ΔmenT4ΔT3 results in increased antigen-specific T
H 1 bias and activated memory T cell response. We conclude that MenT3 and MenT4 are important for M. tuberculosis pathogenicity and strains lacking menT3 and menT4 have the potential to be explored further as vaccine candidates. Gosain et al.'s work aims to enhance understanding of the contribution of MenT3 and MenT4 toxins belonging to MenAT TA systems to the physiology and pathogenesis of Mycobacterium tuberculosis. The authors show that strains lacking these proteins is attenuated and confers protection in vivo. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
37. Expression of tumor antigens within an oncolytic virus enhances the anti-tumor T cell response.
- Author
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Webb, Mason J., Sangsuwannukul, Thanich, van Vloten, Jacob, Evgin, Laura, Kendall, Benjamin, Tonne, Jason, Thompson, Jill, Metko, Muriel, Moore, Madelyn, Chiriboga Yerovi, Maria P., Olin, Michael, Borgatti, Antonella, McNiven, Mark, Monga, Satdarshan P. S., Borad, Mitesh J., Melcher, Alan, Roberts, Lewis R., and Vile, Richard
- Subjects
T cells ,TUMOR antigens ,IMMUNE checkpoint inhibitors ,ONCOLYTIC virotherapy ,VESICULAR stomatitis ,CELL populations ,T cell receptors - Abstract
Although patients benefit from immune checkpoint inhibition (ICI) therapy in a broad variety of tumors, resistance may arise from immune suppressive tumor microenvironments (TME), which is particularly true of hepatocellular carcinoma (HCC). Since oncolytic viruses (OV) can generate a highly immune-infiltrated, inflammatory TME, OVs could potentially restore ICI responsiveness via recruitment, priming, and activation of anti-tumor T cells. Here we find that on the contrary, an oncolytic vesicular stomatitis virus, expressing interferon-ß (VSV-IFNß), antagonizes the effect of anti-PD-L1 therapy in a partially anti-PD-L1-responsive model of HCC. Cytometry by Time of Flight shows that VSV-IFNß expands dominant anti-viral effector CD8 T cells with concomitant relative disappearance of anti-tumor T cell populations, which are the target of anti-PD-L1. However, by expressing a range of HCC tumor antigens within VSV, combination OV and anti-PD-L1 therapeutic benefit could be restored. Our data provide a cautionary message for the use of highly immunogenic viruses as tumor-specific immune-therapeutics by showing that dominant anti-viral T cell responses can inhibit sub-dominant anti-tumor T cell responses. However, through encoding tumor antigens within the virus, oncolytic virotherapy can generate anti-tumor T cell populations upon which immune checkpoint blockade can effectively work. Oncolytic viruses create an inflamed tumour microenvironment allowing T cells to respond to immune checkpoint blockade therapy more efficiently. Authors here show that in a hepatocellular carcinoma model, a dominant anti-viral rather than anti-tumour T cell response is elicited by an oncolytic vesicular stomatitis virus, unless the virus is designed to express tumour antigens, which restores therapeutic benefit. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
38. IFNγ causes mitochondrial dysfunction and oxidative stress in myositis.
- Author
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Abad, Catalina, Pinal-Fernandez, Iago, Guillou, Clement, Bourdenet, Gwladys, Drouot, Laurent, Cosette, Pascal, Giannini, Margherita, Debrut, Lea, Jean, Laetitia, Bernard, Sophie, Genty, Damien, Zoubairi, Rachid, Remy-Jouet, Isabelle, Geny, Bernard, Boitard, Christian, Mammen, Andrew, Meyer, Alain, and Boyer, Olivier
- Subjects
T cells ,MYOSITIS ,MITOCHONDRIA ,OXIDATIVE stress ,REACTIVE oxygen species ,IDIOPATHIC diseases ,AUTOIMMUNE diseases - Abstract
Idiopathic inflammatory myopathies (IIMs) are severe autoimmune diseases with poorly understood pathogenesis and unmet medical needs. Here, we examine the role of interferon γ (IFNγ) using NOD female mice deficient in the inducible T cell co-stimulator (Icos), which have previously been shown to develop spontaneous IFNγ-driven myositis mimicking human disease. Using muscle proteomic and spatial transcriptomic analyses we reveal profound myofiber metabolic dysregulation in these mice. In addition, we report muscle mitochondrial abnormalities and oxidative stress in diseased mice. Supporting a pathogenic role for oxidative stress, treatment with a reactive oxygen species (ROS) buffer compound alleviated myositis, preserved muscle mitochondrial ultrastructure and respiration, and reduced inflammation. Mitochondrial anomalies and oxidative stress were diminished following anti-IFNγ treatment. Further transcriptomic analysis in IIMs patients and human myoblast in vitro studies supported the link between IFNγ and mitochondrial dysfunction observed in mice. These results suggest that mitochondrial dysfunction, ROS and inflammation are interconnected in a self-maintenance loop, opening perspectives for mitochondria therapy and/or ROS targeting drugs in myositis. Idiopathic inflammatory myopathies are severe autoimmune diseases with poorly understood pathogenesis. In this study, the authors use Icos-deficient NOD mice as a model for myositis, as well as clinical samples, to demonstrate mitochondrial abnormalities and metabolic dysfunction, which can be reversed by treatment with the ROS scavenger, N-acetylcysteine (NAC). [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
39. Myelin-reactive B cells exacerbate CD4+ T cell-driven CNS autoimmunity in an IL-23-dependent manner.
- Author
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Fazazi, Mohamed Reda, Doss, Prenitha Mercy Ignatius Arokia, Pereira, Resel, Fudge, Neva, Regmi, Aryan, Joly-Beauparlant, Charles, Akbar, Irshad, Yeola, Asmita Pradeep, Mailhot, Benoit, Baillargeon, Joanie, Grenier, Philippe, Bertrand, Nicolas, Lacroix, Steve, Droit, Arnaud, Moore, Craig S., Rojas, Olga L., and Rangachari, Manu
- Subjects
B cells ,T cells ,T helper cells ,MYELIN oligodendrocyte glycoprotein ,DURA mater ,B cell receptors ,REACTIVE oxygen species ,AUTOIMMUNITY - Abstract
B cells and T cells collaborate in multiple sclerosis (MS) pathogenesis. IgH
[MOG] mice possess a B cell repertoire skewed to recognize myelin oligodendrocyte glycoprotein (MOG). Here, we show that upon immunization with the T cell-obligate autoantigen, MOG[35-55] , IgH[MOG] mice develop rapid and exacerbated experimental autoimmune encephalomyelitis (EAE) relative to wildtype (WT) counterparts, characterized by aggregation of T and B cells in the IgH[MOG] meninges and by CD4+ T helper 17 (Th17) cells in the CNS. Production of the Th17 maintenance factor IL-23 is observed from IgH[MOG] CNS-infiltrating and meningeal B cells, and in vivo blockade of IL-23p19 attenuates disease severity in IgH[MOG] mice. In the CNS parenchyma and dura mater of IgH[MOG] mice, we observe an increased frequency of CD4+ PD-1+ CXCR5- T cells that share numerous characteristics with the recently described T peripheral helper (Tph) cell subset. Further, CNS-infiltrating B and Tph cells from IgH[MOG] mice show increased reactive oxygen species (ROS) production. Meningeal inflammation, Tph-like cell accumulation in the CNS and B/Tph cell production of ROS were all reduced upon p19 blockade. Altogether, MOG-specific B cells promote autoimmune inflammation of the CNS parenchyma and meninges in an IL-23-dependent manner. B cells are crucial in multiple sclerosis (MS) pathology but the mechanisms are incompletely understood. In a mouse model of MS, the authors show that B cells make IL-23, and that IL-23 invokes meningeal inflammation and CNS presence of T peripheral helper cells. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
40. The tyrosine kinase KDR is essential for the survival of HTLV-1-infected T cells by stabilizing the Tax oncoprotein.
- Author
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Mohanty, Suchitra, Suklabaidya, Sujit, Lavorgna, Alfonso, Ueno, Takaharu, Fujisawa, Jun-ichi, Ngouth, Nyater, Jacobson, Steven, and Harhaj, Edward W.
- Subjects
T cells ,PROTEIN-tyrosine kinases ,HTLV-I ,ADULT T-cell leukemia ,CELL transformation ,BOVINE viral diarrhea - Abstract
Human T-cell leukemia virus type 1 (HTLV-1) infection is linked to the development of adult T-cell leukemia/lymphoma (ATLL) and the neuroinflammatory disease, HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). The HTLV-1 Tax oncoprotein regulates viral gene expression and persistently activates NF-κB to maintain the viability of HTLV-1-infected T cells. Here, we utilize a kinome-wide shRNA screen to identify the tyrosine kinase KDR as an essential survival factor of HTLV-1-transformed cells. Inhibition of KDR specifically induces apoptosis of Tax expressing HTLV-1-transformed cell lines and CD4 + T cells from HAM/TSP patients. Furthermore, inhibition of KDR triggers the autophagic degradation of Tax resulting in impaired NF-κB activation and diminished viral transmission in co-culture assays. Tax induces the expression of KDR, forms a complex with KDR, and is phosphorylated by KDR. These findings suggest that Tax stability is dependent on KDR activity which could be exploited as a strategy to target Tax in HTLV-1-associated diseases. The human T-cell leukemia virus type 1 Tax oncoprotein plays essential roles in regulating viral gene expression and cell survival. Here, the authors show that Tax requires the tyrosine kinase KDR to prevent its degradation by autophagy. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
41. Viral modulation of type II interferon increases T cell adhesion and virus spread.
- Author
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Jacobsen, Carina, Plückebaum, Nina, Ssebyatika, George, Beyer, Sarah, Mendes-Monteiro, Lucas, Wang, Jiayi, Kropp, Kai A., González-Motos, Víctor, Steinbrück, Lars, Ritter, Birgit, Rodríguez-González, Claudio, Böning, Heike, Nikolouli, Eirini, Kinchington, Paul R., Lachmann, Nico, Depledge, Daniel P., Krey, Thomas, and Viejo-Borbolla, Abel
- Subjects
INTERFERON gamma ,T cells ,CELL adhesion ,MONONUCLEAR leukocytes ,INTERFERON receptors ,CD54 antigen ,VIRAL transmission ,EPITHELIAL cells - Abstract
During primary varicella zoster virus (VZV) infection, infected lymphocytes drive primary viremia, causing systemic dissemination throughout the host, including the skin. This results in cytokine expression, including interferons (IFNs), which partly limit infection. VZV also spreads from skin keratinocytes to lymphocytes prior to secondary viremia. It is not clear how VZV achieves this while evading the cytokine response. Here, we show that VZV glycoprotein C (gC) binds IFN-γ and modifies its activity, increasing the expression of a subset of IFN-stimulated genes (ISGs), including intercellular adhesion molecule 1 (ICAM1), chemokines and immunomodulatory genes. The higher ICAM1 protein level at the plasma membrane of keratinocytes facilitates lymphocyte function-associated antigen 1-dependent T cell adhesion and expression of gC during infection increases VZV spread to peripheral blood mononuclear cells. This constitutes the discovery of a strategy to modulate IFN-γ activity, upregulating a subset of ISGs, promoting enhanced lymphocyte adhesion and virus spread. Here, Jacobsen et al show that Varicella zoster virus glycoprotein C binds interferon gamma and induces biased signalling through its receptor, leading to higher expression of adhesion molecules, more T cell adhesion and viral spread from epithelial cells. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
42. Viral modulation of type II interferon increases T cell adhesion and virus spread.
- Author
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Jacobsen, Carina, Plückebaum, Nina, Ssebyatika, George, Beyer, Sarah, Mendes-Monteiro, Lucas, Wang, Jiayi, Kropp, Kai A., González-Motos, Víctor, Steinbrück, Lars, Ritter, Birgit, Rodríguez-González, Claudio, Böning, Heike, Nikolouli, Eirini, Kinchington, Paul R., Lachmann, Nico, Depledge, Daniel P., Krey, Thomas, and Viejo-Borbolla, Abel
- Subjects
INTERFERON gamma ,T cells ,CELL adhesion ,MONONUCLEAR leukocytes ,INTERFERON receptors ,CD54 antigen ,VIRAL transmission ,EPITHELIAL cells - Abstract
During primary varicella zoster virus (VZV) infection, infected lymphocytes drive primary viremia, causing systemic dissemination throughout the host, including the skin. This results in cytokine expression, including interferons (IFNs), which partly limit infection. VZV also spreads from skin keratinocytes to lymphocytes prior to secondary viremia. It is not clear how VZV achieves this while evading the cytokine response. Here, we show that VZV glycoprotein C (gC) binds IFN-γ and modifies its activity, increasing the expression of a subset of IFN-stimulated genes (ISGs), including intercellular adhesion molecule 1 (ICAM1), chemokines and immunomodulatory genes. The higher ICAM1 protein level at the plasma membrane of keratinocytes facilitates lymphocyte function-associated antigen 1-dependent T cell adhesion and expression of gC during infection increases VZV spread to peripheral blood mononuclear cells. This constitutes the discovery of a strategy to modulate IFN-γ activity, upregulating a subset of ISGs, promoting enhanced lymphocyte adhesion and virus spread. Here, Jacobsen et al show that Varicella zoster virus glycoprotein C binds interferon gamma and induces biased signalling through its receptor, leading to higher expression of adhesion molecules, more T cell adhesion and viral spread from epithelial cells. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
43. CD8+ T cell-derived Fgl2 regulates immunity in a cell-autonomous manner via ligation of FcγRIIB.
- Author
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Bennion, Kelsey B., Liu, Danya, Dawood, Abdelhameed S., Wyatt, Megan M., Alexander, Katie L., Abdel-Hakeem, Mohamed S., Paulos, Chrystal M., and Ford, Mandy L.
- Subjects
T cells ,T-cell exhaustion ,IMMUNITY ,CYTOKINE receptors - Abstract
The regulatory circuits dictating CD8
+ T cell responsiveness versus exhaustion during anti-tumor immunity are incompletely understood. Here we report that tumor-infiltrating antigen-specific PD-1+ TCF-1− CD8+ T cells express the immunosuppressive cytokine Fgl2. Conditional deletion of Fgl2 specifically in mouse antigen-specific CD8+ T cells prolongs CD8+ T cell persistence, suppresses phenotypic and transcriptomic signatures of T cell exhaustion, and improves control of the tumor. In a mouse model of chronic viral infection, PD-1+ CD8+ T cell-derived Fgl2 also negatively regulates virus-specific T cell responses. In humans, CD8+ T cell-derived Fgl2 is associated with poorer survival in patients with melanoma. Mechanistically, the dampened responsiveness of WT Fgl2-expressing CD8+ T cells, when compared to Fgl2-deficient CD8+ T cells, is underpinned by the cell-intrinsic interaction of Fgl2 with CD8+ T cell-expressed FcγRIIB and concomitant caspase 3/7-mediated apoptosis. Our results thus illuminate a cell-autonomous regulatory axis by which PD-1+ CD8+ T cells both express the receptor and secrete its ligand in order to mediate suppression of anti-tumor and anti-viral immunity. Mechanisms regulating CD8 T cell responsiveness are not fully understood. Here the authors show, using mouse genetic tools and human patient samples, that CD8 T-derived Fgl2, an immunosuppressive cytokine, binds autologous FcγRIIB receptor to induce CD8 T cell apoptosis and dampen anti-tumor or antivirus immunity. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
44. E proteins control the development of NKγδT cells through their invariant T cell receptor.
- Author
-
Mihai, Ariana, Lee, Sang-Yun, Shinton, Susan, Parker, Mitchell I., Contreras, Alejandra V., Zhang, Baojun, Rhodes, Michele, Dunbrack, Roland L., Zúñiga-Pflücker, Juan-Carlos, Ciofani, Maria, Zhuang, Yuan, and Wiest, David L.
- Subjects
T cell receptors ,T cells ,CAPACITY building ,PROTEINS ,CARRIER proteins - Abstract
T cell receptor (TCR) signaling regulates important developmental transitions, partly through induction of the E protein antagonist, Id3. Although normal γδ T cell development depends on Id3, Id3 deficiency produces different phenotypes in distinct γδ T cell subsets. Here, we show that Id3 deficiency impairs development of the Vγ3
+ subset, while markedly enhancing development of NKγδT cells expressing the invariant Vγ1Vδ6.3 TCR. These effects result from Id3 regulating both the generation of the Vγ1Vδ6.3 TCR and its capacity to support development. Indeed, the Trav15 segment, which encodes the Vδ6.3 TCR subunit, is directly bound by E proteins that control its expression. Once expressed, the Vγ1Vδ6.3 TCR specifies the innate-like NKγδT cell fate, even in progenitors beyond the normally permissive perinatal window, and this is enhanced by Id3-deficiency. These data indicate that the paradoxical behavior of NKγδT cells in Id3-deficient mice is determined by its stereotypic Vγ1Vδ6.3 TCR complex. Although normal γδ T cell development depends on the E protein antagonist, Id3, Id3 deficiency produces different phenotypes in distinct γδ T cell subsets. Here, the authors demonstrate that Id3 deficiency impairs the development of Vγ3+ T cells, while promoting that of NKγδT cells expressing the invariant Vγ1Vδ6.3 TCR. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
45. SLAM-family receptors promote resolution of ILC2-mediated inflammation.
- Author
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Wang, Yuande, Quan, Yuhe, He, Junming, Chen, Shasha, and Dong, Zhongjun
- Subjects
INNATE lymphoid cells ,TH2 cells ,INFLAMMATION ,IMMUNE response ,PNEUMONIA ,T cells ,EOSINOPHILIA - Abstract
Type 2 innate lymphoid cells (ILC2) initiate early allergic inflammation in the lung, but the factors that promote subsequent resolution of type 2 inflammation and prevent prolonged ILC2 activation are not fully known. Here we show that SLAM-family receptors (SFR) play essential roles in this process. We demonstrate dynamic expression of several SFRs on ILC2s during papain-induced type 2 immunity in mice. SFR deficiency exacerbates ILC2-driven eosinophil infiltration in the lung, and results in a significant increase in IL-13 production by ILC2s exclusively in mediastinal lymph nodes (MLN), leading to increased dendritic cell (DC) and TH2 cell numbers. In MLNs, we observe more frequent interaction between ILC2s and bystander T cells, with T cell-expressed SFRs (especially SLAMF3 and SLAMF5) acting as self-ligands to suppress IL-13 production by ILC2s. Mechanistically, homotypic engagement of SFRs at the interface between ILC2s and T cells delivers inhibitory signaling primarily mediated by SHIP-1. This prevents activation of NF-κB, driven by IL-7 and IL-33, two major drivers of ILC2-mediated type 2 immunity. Thus, our study shows that an ILC2-DC-TH2 regulatory axis may promote the resolution of pulmonary type 2 immune responses, and highlights SLAMF3/SLAMF5 as potential therapeutic targets for ameliorating type 2 immunity. Type 2 innate lymphoid cells are instrumental to the early phases of pulmonary allergic inflammation, but the negative regulation that leads to their inhibition in the resolution phase is less known. Here authors show that SLAM-family receptors control Il-13 production and NF-κB activation in the mediastinal lymph nodes but not in the lung, eventually setting a limit to lung inflammation. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
46. Germline-like TCR-α chains shared between autoreactive T cells in blood and pancreas.
- Author
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Linsley, Peter S., Nakayama, Maki, Balmas, Elisa, Chen, Janice, Barahmand-pour-Whitman, Fariba, Bansal, Shubham, Bottorff, Ty, Serti, Elisavet, Speake, Cate, Pugliese, Alberto, and Cerosaletti, Karen
- Subjects
T cells ,BLOOD cells ,T cell receptors ,PANCREATIC beta cells ,DEAD ,AMINO acid sequence ,TYPE 1 diabetes ,PANCREAS - Abstract
Human type 1 diabetes (T1D) is caused by autoimmune attack on the insulin-producing pancreatic beta cells by islet antigen-reactive T cells. How human islet antigen-reactive (IAR) CD4+ memory T cells from peripheral blood affect T1D progression in the pancreas is poorly understood. Here, we aim to determine if IAR T cells in blood could be detected in pancreas. We identify paired αβ (TRA/TRB) T cell receptors (TCRs) in IAR T cells from the blood of healthy, at-risk, new-onset, and established T1D donors, and measured sequence overlap with TCRs in pancreata from healthy, at risk and T1D organ donors. We report extensive TRA junction sharing between IAR T cells and pancreas-infiltrating T cells (PIT), with perfect-match or single-mismatch TRA junction amino acid sequences comprising ~29% total unique IAR TRA junctions (942/3,264). PIT-matched TRA junctions were largely public and enriched for TRAV41 usage, showing significant nucleotide sequence convergence, increased use of germline-encoded versus non-templated residues in epitope engagement, and a potential for cross-reactivity. Our findings thus link T cells with distinctive germline-like TRA chains in the peripheral blood with T cells in the pancreas. Most efforts to examine islet antigen-reactive T cells in type-1 diabetes have been limited to peripheral blood. In this study, the authors examine the T cell receptor (TCR) repertoire of insulin antigen-reactive T cells from peripheral blood from patients with type-1 diabetes as well as pancreata from deceased donors. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
47. Immunological synapse formation between T regulatory cells and cancer-associated fibroblasts promotes tumour development.
- Author
-
Varveri, Athina, Papadopoulou, Miranta, Papadovasilakis, Zacharias, Compeer, Ewoud B., Legaki, Aigli-Ioanna, Delis, Anastasios, Damaskou, Vasileia, Boon, Louis, Papadogiorgaki, Sevasti, Samiotaki, Martina, Foukas, Periklis G., Eliopoulos, Aristides G., Hatzioannou, Aikaterini, Alissafi, Themis, Dustin, Michael L., and Verginis, Panayotis
- Subjects
REGULATORY T cells ,T cells ,SYNAPTOGENESIS ,FIBROBLASTS ,CELL populations ,ANTIGEN processing - Abstract
Cancer-associated fibroblasts (CAFs) have emerged as a dominant non-hematopoietic cell population in the tumour microenvironment, serving diverse functions in tumour progression. However, the mechanisms via which CAFs influence the anti-tumour immunity remain poorly understood. Here, using multiple tumour models and biopsies from cancer patients, we report that α-SMA
+ CAFs can form immunological synapses with Foxp3+ regulatory T cells (Tregs) in tumours. Notably, α-SMA+ CAFs can phagocytose and process tumour antigens and exhibit a tolerogenic phenotype which instructs movement arrest, activation and proliferation in Tregs in an antigen-specific manner. Moreover, α-SMA+ CAFs display double-membrane structures resembling autophagosomes in their cytoplasm. Single-cell transcriptomic data showed an enrichment in autophagy and antigen processing/presentation pathways in α-SMA-expressing CAF clusters. Conditional knockout of Atg5 in α-SMA+ CAFs promoted inflammatory re-programming in CAFs, reduced Treg cell infiltration and attenuated tumour development. Overall, our findings reveal an immunosuppressive mechanism entailing the formation of synapses between α-SMA+ CAFs and Tregs in an autophagy-dependent manner. Cancer-associated fibroblasts (CAFs) are a predominant stromal cell population in the tumour microenvironment. Here, the authors demonstrate that αSMA + CAFs can form an immunological synapse with regulatory T cells (Tregs) in tumours, which results in Treg activation and expansion in a process that is antigen- and autophagy-dependent. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
48. Single-cell multi-ome and immune profiles of the Inspiration4 crew reveal conserved, cell-type, and sex-specific responses to spaceflight.
- Author
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Kim, JangKeun, Tierney, Braden T., Overbey, Eliah G., Dantas, Ezequiel, Fuentealba, Matias, Park, Jiwoon, Narayanan, S. Anand, Wu, Fei, Najjar, Deena, Chin, Christopher R., Meydan, Cem, Loy, Conor, Mathyk, Begum, Klotz, Remi, Ortiz, Veronica, Nguyen, Khiem, Ryon, Krista A., Damle, Namita, Houerbi, Nadia, and Patras, Laura I.
- Subjects
SPACE flight ,T cells ,HEALTH of astronauts ,OXIDATIVE phosphorylation ,TRANSCRIPTOMES ,GENE expression ,CHEMOKINE receptors - Abstract
Spaceflight induces an immune response in astronauts. To better characterize this effect, we generated single-cell, multi-ome, cell-free RNA (cfRNA), biochemical, and hematology data for the SpaceX Inspiration4 (I4) mission crew. We found that 18 cytokines/chemokines related to inflammation, aging, and muscle homeostasis changed after spaceflight. In I4 single-cell multi-omics data, we identified a "spaceflight signature" of gene expression characterized by enrichment in oxidative phosphorylation, UV response, immune function, and TCF21 pathways. We confirmed the presence of this signature in independent datasets, including the NASA Twins Study, the I4 skin spatial transcriptomics, and 817 NASA GeneLab mouse transcriptomes. Finally, we observed that (1) T cells showed an up-regulation of FOXP3, (2) MHC class I genes exhibited long-term suppression, and (3) infection-related immune pathways were associated with microbiome shifts. In summary, this study reveals conserved and distinct immune disruptions occurring and details a roadmap for potential countermeasures to preserve astronaut health. Multiple omics platforms and deep single-cell profiling in the I4 astronauts reveal both conserved and distinct immune system disruptions across missions, provide a single-cell immune reference for future missions. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
49. Clonal associations between lymphocyte subsets and functional states in rheumatoid arthritis synovium.
- Author
-
Dunlap, Garrett, Wagner, Aaron, Meednu, Nida, Wang, Ruoqiao, Zhang, Fan, Ekabe, Jabea Cyril, Jonsson, Anna Helena, Wei, Kevin, Sakaue, Saori, Nathan, Aparna, Accelerating Medicines Partnership Program: Rheumatoid Arthritis and Systemic Lupus Erythematosus (AMP RA/SLE) Network, Albrecht, Jennifer, Apruzzese, William, Barnas, Jennifer L., Bathon, Joan M., Ben-Artzi, Ami, Boyce, Brendan F., Bridges Jr, S. Louis, Campbell, Debbie, and Carr, Hayley L.
- Subjects
LYMPHOCYTE subsets ,RHEUMATOID arthritis ,T helper cells ,T cells ,SYNOVIAL membranes ,T cell receptors - Abstract
Rheumatoid arthritis (RA) is an autoimmune disease involving antigen-specific T and B cells. Here, we perform single-cell RNA and repertoire sequencing on paired synovial tissue and blood samples from 12 seropositive RA patients. We identify clonally expanded CD4 + T cells, including CCL5+ cells and T peripheral helper (Tph) cells, which show a prominent transcriptomic signature of recent activation and effector function. CD8 + T cells show higher oligoclonality than CD4 + T cells, with the largest synovial clones enriched in GZMK+ cells. CD8 + T cells with possibly virus-reactive TCRs are distributed across transcriptomic clusters. In the B cell compartment, NR4A1+ activated B cells, and plasma cells are enriched in the synovium and demonstrate substantial clonal expansion. We identify synovial plasma cells that share BCRs with synovial ABC, memory, and activated B cells. Receptor-ligand analysis predicted IFNG and TNFRSF members as mediators of synovial Tph-B cell interactions. Together, these results reveal clonal relationships between functionally distinct lymphocyte populations that infiltrate the synovium of patients with RA. Activated B cells and T cells accumulate within joints of patients with rheumatoid arthritis. Here, the authors use single-cell transcriptome and repertoire profiling to identify clonally expanded synovial B cells and T cells and define their phenotypes and predicted cell-cell interactions. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
50. SOD3 suppresses early cellular immune responses to parasite infection.
- Author
-
Li, Qilong, Lv, Kunying, Jiang, Ning, Liu, Tong, Hou, Nan, Yu, Liying, Yang, Yixin, Feng, Anni, Zhang, Yiwei, Su, Ziwei, Sang, Xiaoyu, Feng, Ying, Chen, Ran, Xu, Wenyue, Cui, Liwang, Cao, Yaming, and Chen, Qijun
- Subjects
IMMUNE response ,T cells ,PLASMODIUM ,CEREBRAL malaria ,PARASITES ,SUPEROXIDE dismutase ,ARMS race ,PLASMODIUM falciparum - Abstract
Host immune responses are tightly controlled by various immune factors during infection, and protozoan parasites also manipulate the immune system to evade surveillance, leading to an evolutionary arms race in host‒pathogen interactions; however, the underlying mechanisms are not fully understood. We observed that the level of superoxide dismutase 3 (SOD3) was significantly elevated in both Plasmodium falciparum malaria patients and mice infected with four parasite species. SOD3-deficient mice had a substantially longer survival time and lower parasitemia than control mice after infection, whereas SOD3-overexpressing mice were much more vulnerable to parasite infection. We revealed that SOD3, secreted from activated neutrophils, bound to T cells, suppressed the interleukin-2 expression and concomitant interferon-gamma responses crucial for parasite clearance. Overall, our findings expose active fronts in the arms race between the parasites and host immune system and provide insights into the roles of SOD3 in shaping host innate immune responses to parasite infection. Superoxide dismutase 3 is elevated in patients and animals infected by protozoan parasites such as Plasmodium. Here, Li et al. show that SOD3 expression is associated with experimental cerebral malaria and inhibition of host immune responses. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
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