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Your search keyword '"Dahl, Andreas"' showing total 6 results
Start Over Author "Dahl, Andreas" Journal nature communications
6 results on '"Dahl, Andreas"'

2. Targeting cardiomyocyte ADAM10 ectodomain shedding promotes survival early after myocardial infarction

Author

Klapproth, Erik, Witt, Anke, Klose, Pauline, Wiedemann, Johanna, Vavilthota, Nikitha, Künzel, Stephan R., Kämmerer, Susanne, Günscht, Mario, Sprott, David, Lesche, Mathias, Rost, Fabian, Dahl, Andreas, Rauch, Erik, Kattner, Lars, Weber, Silvio, Mirtschink, Peter, Kopaliani, Irakli, Guan, Kaomei, Lorenz, Kristina, Saftig, Paul, Wagner, Michael, and El-Armouche, Ali

Published
2022
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5. Epigenomic map of human liver reveals principles of zonated morphogenic and metabolic control

Author

Brosch, Mario, Kattler, Kathrin, Herrmann, Alexander, von Schönfels, Witigo, Nordström, Karl, Seehofer, Daniel, Damm, Georg, Becker, Thomas, Zeissig, Sebastian, Nehring, Sophie, Reichel, Fabian, Moser, Vincent, Thangapandi, Raghavan Veera, Stickel, Felix, Baretton, Gustavo, Röcken, Christoph, Muders, Michael, Matz-Soja, Madlen, Krawczak, Michael, Gasparoni, Gilles, Hartmann, Hella, Dahl, Andreas, Schafmayer, Clemens, Walter, Jörn, and Hampe, Jochen

Published
2018
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6. Cre-Controlled CRISPR mutagenesis provides fast and easy conditional gene inactivation in zebrafish

Author

Hans, Stefan, Zöller, Daniela, Hammer, Juliane, Stucke, Johanna, Spieß, Sandra, Kesavan, Gokul, Kroehne, Volker, Eguiguren, Juan Sebastian, Ezhkova, Diana, Petzold, Andreas, Dahl, Andreas, and Brand, Michael

Subjects
CRISPR-Cas9 genome editing, Time Factors, Base Sequence, Integrases, Molecular engineering, Monophenol Monooxygenase, Pigmentation, Science, Green Fluorescent Proteins, Eye, Article, Mutagenesis, Differentiation, Animals, Clustered Regularly Interspaced Short Palindromic Repeats, Gene Silencing, RNA, Messenger, Transgenes, Zebrafish
Abstract

Conditional gene inactivation is a powerful tool to determine gene function when constitutive mutations result in detrimental effects. The most commonly used technique to achieve conditional gene inactivation employs the Cre/loxP system and its ability to delete DNA sequences flanked by two loxP sites. However, targeting a gene with two loxP sites is time and labor consuming. Here, we show Cre-Controlled CRISPR (3C) mutagenesis to circumvent these issues. 3C relies on gRNA and Cre-dependent Cas9-GFP expression from the same transgene. Exogenous or transgenic supply of Cre results in Cas9-GFP expression and subsequent mutagenesis of the gene of interest. The recombined cells become fluorescently visible enabling their isolation and subjection to various omics techniques. Hence, 3C mutagenesis provides a valuable alternative to the production of loxP-flanked alleles. It might even enable the conditional inactivation of multiple genes simultaneously and should be applicable to other model organisms amenable to single integration transgenesis., Targeting a gene with two loxP sites is both time and labour intensive. Here the authors present Cre-Controlled CRISPR allowing conditional mutagenesis of a gene of interest and simultaneously labelling the putative mutant cells fluorescently.

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