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Compared to liposomes, polymersomes of block copolymers (BCPs) possess enhanced stability, along with compromised bilayer permeability. Though polyion complex vesicles (PICsomes) from oppositely charged block polyelectrolytes possess semipermeable bilayers, they are unstable towards physiologically relevant ionic strength and temperature; moreover, permselectivity tuning of PICsomes has remained a challenge. Starting from a single component diblock or triblock precursor, we solve this dilemma by stimuli-triggered chemical reactions within pre-organized BCP vesicles, actuating in situ polymersome-to-PICsome transition and achieving molecular size-selective cargo release at tunable rates. UV light and reductive milieu were utilized to trigger carboxyl decaging and generate ion pairs within hydrophobic polymersome bilayers containing tertiary amines. Contrary to conventional PICsomes, in situ generated ones are highly stable towards extreme pH range (pH 2-12), ionic strength (~3 M NaCl), and elevated temperature (70 °C) due to multivalent ion-pair interactions at high local concentration and cooperative hydrogen bonding interactions of pre-organized carbamate linkages., Polyion complex vesicles have advantageous applications but have poorly tuneable permeability and nanostructure stability. Here, the authors develop polyion complex vesicles created using a stimuli-triggered transition from polymersomes and show high stability and switchable bilayer permaselectivity.

A discontinuity in the seismic velocity associated with the lithosphere-asthenosphere interface, known as the Gutenberg discontinuity, is enigmatic in its origin. While partial mantle melts are frequently suggested to explain this discontinuity, it is not well known which factors critically regulate the melt production. Here, we report geochemical evidence showing that the melt fractions in the lithosphere-asthenosphere boundary were enhanced not only by accumulation of compacted carbonated melts related to recycled ancient marine sediments, but also by partial melting of a pyroxene-rich mantle domain related to the recycled oceanic eclogite/pyroxenites. This conclusion is derived from the first set of Mg isotope data for a suite of young petit-spot basalts erupted on the northwest Pacific plate, where a clearly defined Gutenberg discontinuity exists. Our results reveal a specific linkage between the Gutenberg discontinuity beneath the normal oceanic regions and the recycling of ancient subducted crust and carbonate through the deep Earth., The seismic Gutenberg discontinuity has long been associated with the lithosphere-asthenosphere boundary, yet the physical explanation of what is causing the discontinuity remains debated. Here, the authors report geochemical evidence, including Mg isotopes, and suggest that melting of recycled crust is responsible for the Gutenberg discontinuity.

Although it has long been known that patients with sepsis often have thrombocytopenia and that septic patients with severe thrombocytopenia have a poor prognosis and higher mortality, the role of platelets in the pathogenesis of sepsis is poorly understood. Here we report a protective role of platelets in septic shock. We show that experimental thrombocytopenia induced by intraperitoneal injection of an anti-glycoprotein Ibα monoclonal antibody increases mortality and aggravates organ failure, whereas transfusion of platelets reduces mortality in lipopolysaccharide-induced endotoxemia and a bacterial infusion mouse sepsis model. Plasma concentrations of proinflammatory cytokines TNF-α and IL-6 are elevated by thrombocytopenia and decreased by platelet transfusion in septic mice. Furthermore, we identify that platelets protect from septic shock by inhibiting macrophage-dependent inflammation via the COX1/PGE₂/EP4-dependent pathway. Thus, these findings demonstrate a previously unappreciated role for platelets in septic shock and suggest that platelet transfusion may be effective in treating severely septic patients.