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Your search keyword '"Hirabayashi, Masumi"' showing total 6 results
Start Over Author "Hirabayashi, Masumi" Journal nature communications
6 results on '"Hirabayashi, Masumi"'

1. Myocardial TRPC6-mediated Zn2+ influx induces beneficial positive inotropy through β-adrenoceptors

Author

Oda, Sayaka, Nishiyama, Kazuhiro, Furumoto, Yuka, Yamaguchi, Yohei, Nishimura, Akiyuki, Tang, Xiaokang, Kato, Yuri, Numaga-Tomita, Takuro, Kaneko, Toshiyuki, Mangmool, Supachoke, Kuroda, Takuya, Okubo, Reishin, Sanbo, Makoto, Hirabayashi, Masumi, Sato, Yoji, Nakagawa, Yasuaki, Kuwahara, Koichiro, Nagata, Ryu, Iribe, Gentaro, Mori, Yasuo, and Nishida, Motohiro

Published
2022
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4. Myocardial TRPC6-mediated Zn2+ influx induces beneficial positive inotropy through β-adrenoceptors.

Author

Oda, Sayaka, Nishiyama, Kazuhiro, Furumoto, Yuka, Yamaguchi, Yohei, Nishimura, Akiyuki, Tang, Xiaokang, Kato, Yuri, Numaga-Tomita, Takuro, Kaneko, Toshiyuki, Mangmool, Supachoke, Kuroda, Takuya, Okubo, Reishin, Sanbo, Makoto, Hirabayashi, Masumi, Sato, Yoji, Nakagawa, Yasuaki, Kuwahara, Koichiro, Nagata, Ryu, Iribe, Gentaro, and Mori, Yasuo

Subjects
TRP channels, CARDIAC contraction, BAROREFLEXES, HEART failure, AMINO acids, HOMEOSTASIS
Abstract

Baroreflex control of cardiac contraction (positive inotropy) through sympathetic nerve activation is important for cardiocirculatory homeostasis. Transient receptor potential canonical subfamily (TRPC) channels are responsible for α1-adrenoceptor (α1AR)-stimulated cation entry and their upregulation is associated with pathological cardiac remodeling. Whether TRPC channels participate in physiological pump functions remains unclear. We demonstrate that TRPC6-specific Zn2+ influx potentiates β-adrenoceptor (βAR)-stimulated positive inotropy in rodent cardiomyocytes. Deletion of trpc6 impairs sympathetic nerve–activated positive inotropy but not chronotropy in mice. TRPC6-mediated Zn2+ influx boosts α1AR-stimulated βAR/Gs-dependent signaling in rat cardiomyocytes by inhibiting β-arrestin-mediated βAR internalization. Replacing two TRPC6-specific amino acids in the pore region with TRPC3 residues diminishes the α1AR-stimulated Zn2+ influx and positive inotropic response. Pharmacological enhancement of TRPC6-mediated Zn2+ influx prevents chronic heart failure progression in mice. Our data demonstrate that TRPC6-mediated Zn2+ influx with α1AR stimulation enhances baroreflex-induced positive inotropy, which may be a new therapeutic strategy for chronic heart failure. Baroreflex control of cardiac contractility is essential to maintain cardiocirculatory homeostasis. Here, Oda et al show that α1 adrenoceptor-stimulated Zn2+ entry through TRPC6 channels boosts β adrenoceptor-dependent myocardial positive inotropy. [ABSTRACT FROM AUTHOR]

Published
2022
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5. Structural basis of epilepsy-related ligand–receptor complex LGI1–ADAM22

Author

Yamagata, Atsushi, primary, Miyazaki, Yuri, additional, Yokoi, Norihiko, additional, Shigematsu, Hideki, additional, Sato, Yusuke, additional, Goto-Ito, Sakurako, additional, Maeda, Asami, additional, Goto, Teppei, additional, Sanbo, Makoto, additional, Hirabayashi, Masumi, additional, Shirouzu, Mikako, additional, Fukata, Yuko, additional, Fukata, Masaki, additional, and Fukai, Shuya, additional

Published
2018
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6. Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats

Author

Naoyo Kajitani, Shinichi Hochi, Fumika Yoshida, Hiromitsu Nakauchi, Toshihiro Kobayashi, Masumi Hirabayashi, Makoto Sanbo, Teppei Goto, Mami Oikawa, Reiko Terada, Kanako Kazuki, Yasuhiro Kazuki, M. Azim Surani, Naoko Niizeki, Kobayashi, Toshihiro [0000-0001-8019-0008], Sanbo, Makoto [0000-0001-8806-1745], Hochi, Shinichi [0000-0001-5315-3471], Nakauchi, Hiromitsu [0000-0002-9841-6973], Surani, M. Azim [0000-0002-8640-4318], Hirabayashi, Masumi [0000-0002-1059-5883], Apollo - University of Cambridge Repository, and Surani, M Azim [0000-0002-8640-4318]

Subjects
0301 basic medicine, Male, Knockout rat, Somatic cell, Germline development, 42/109, General Physics and Astronomy, Germline, 631/532/2117, Gene Knockout Techniques, Mice, 0302 clinical medicine, 13/44, Induced pluripotent stem cell, 631/136/2434, Multidisciplinary, RNA-Binding Proteins, Spermatids, Cell biology, Genetically modified organism, 13/31, DNA-Binding Proteins, medicine.anatomical_structure, Models, Animal, 38/77, 64/60, Female, Pluripotent Stem Cells, Embryonic stem cells, Science, 631/532/2064, Biology, General Biochemistry, Genetics and Molecular Biology, Article, 03 medical and health sciences, 13/100, medicine, Animals, Blastocyst, General Chemistry, Embryonic stem cell, Rats, 030104 developmental biology, Germ Cells, 64/86, 13/51, Genetic engineering, 631/61/17/1511, Transcriptome, 030217 neurology & neurosurgery, Transcription Factors
Abstract

Murine animal models from genetically modified pluripotent stem cells (PSCs) are essential for functional genomics and biomedical research, which require germline transmission for the establishment of colonies. However, the quality of PSCs, and donor-host cell competition in chimeras often present strong barriers for germline transmission. Here, we report efficient germline transmission of recalcitrant PSCs via blastocyst complementation, a method to compensate for missing tissues or organs in genetically modified animals via blastocyst injection of PSCs. We show that blastocysts from germline-deficient Prdm14 knockout rats provide a niche for the development of gametes originating entirely from the donor PSCs without any detriment to somatic development. We demonstrate the potential of this approach by creating PSC-derived Pax2/Pax8 double mutant anephric rats, and rescuing germline transmission of a PSC carrying a mouse artificial chromosome. Furthermore, we generate mouse PSC-derived functional spermatids in rats, which provides a proof-of-principle for the generation of xenogenic gametes in vivo. We believe this approach will become a useful system for generating PSC-derived germ cells in the future., The uptake of donor pluripotent stem cells (PSCs) in hosts of different species and subsequent germline transmission is very inefficient. Here, the authors show, using Prdm14 gene depleted rat host blastocysts to remove functional sperm, that germline transmission from donor rat or mouse PSCs is possible.

Published
2021

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