4 results on '"Philippe Coulombe"'
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2. The ORC ubiquitin ligase OBI1 promotes DNA replication origin firing
- Author
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Philippe Coulombe, Joelle Nassar, Isabelle Peiffer, Slavica Stanojcic, Yvon Sterkers, Axel Delamarre, Stéphane Bocquet, and Marcel Méchali
- Subjects
Science - Abstract
DNA replication is initiated at defined genomic sites called origins of replication following ORC pre-replicative complex assembly. Here the authors identify a protein ubiquitylating ORC that is involved in origin activation and may act as a selector of origins to be fired.
- Published
- 2019
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3. Author Correction: Involvement of G-quadruplex regions in mammalian replication origin activity
- Author
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Paulina Prorok, Marie Artufel, Antoine Aze, Philippe Coulombe, Isabelle Peiffer, Laurent Lacroix, Aurore Guédin, Jean-Louis Mergny, Julia Damaschke, Aloys Schepers, Christelle Cayrou, Marie-Paule Teulade-Fichou, Benoit Ballester, and Marcel Méchali
- Subjects
Science - Abstract
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
- Published
- 2020
- Full Text
- View/download PDF
4. Involvement of G-quadruplex regions in mammalian replication origin activity
- Author
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Paulina Prorok, Aurore Guédin, Isabelle Peiffer, Aloys Schepers, Marcel Méchali, Christelle Cayrou, Marie Artufel, Jean-Louis Mergny, Antoine Aze, Laurent Lacroix, Benoit Ballester, Philippe Coulombe, Marie-Paule Teulade-Fichou, Julia Damaschke, Centre de Recherche en Cancérologie de Marseille (CRCM), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Aix Marseille Université (AMU), COULOMBE, Philippe, Appel à projets générique - Le choix des origines de réplication dans le contrôle de la stabilité génomique et l'identité cellulaire - - ORICHOICE2014 - ANR-14-CE10-0019 - Appel à projets générique - VALID, JIETSSP: Cost Effective, Efficient Monitoring and Control of Space Solar Power Management - 2002-09-01 - 2009-08-31 - 0233339 - VALID, Institut de génétique humaine (IGH), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Theories and Approaches of Genomic Complexity (TAGC), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), University of Cambridge [UK] (CAM), Acides Nucléiques : Régulations Naturelle et Artificielle (ARNA), Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut Européen de Chimie et Biologie (IECB), Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Research Unit Gene Vectors, Helmholtz Zentrum München = German Research Center for Environmental Health, Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut Curie [Paris], ANR-14-CE10-0019,ORICHOICE,Le choix des origines de réplication dans le contrôle de la stabilité génomique et l'identité cellulaire(2014), European Project: 0233339(2002), Mergny, Jean-Louis [0000-0003-3043-8401], Ballester, Benoit [0000-0002-0834-7135], Apollo - University of Cambridge Repository, Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Helmholtz-Zentrum München (HZM)
- Subjects
0301 basic medicine ,Molecular biology ,[SDV]Life Sciences [q-bio] ,Xenopus ,General Physics and Astronomy ,02 engineering and technology ,medicine.disease_cause ,Mice ,Xenopus laevis ,heterocyclic compounds ,lcsh:Science ,ComputingMilieux_MISCELLANEOUS ,Cells, Cultured ,Mammals ,Mutation ,Multidisciplinary ,biology ,021001 nanoscience & nanotechnology ,Ligand (biochemistry) ,[SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM] ,Chromatin ,Cell biology ,[SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,0210 nano-technology ,Origin selection ,Plasmids ,DNA Replication ,DNA replication initiation ,Science ,Genetic Vectors ,Replication Origin ,G-quadruplex ,Article ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,medicine ,Animals ,Humans ,Author Correction ,DNA replication ,General Chemistry ,biology.organism_classification ,In vitro ,G-Quadruplexes ,030104 developmental biology ,[SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics ,NIH 3T3 Cells ,Oocytes ,lcsh:Q - Abstract
Genome-wide studies of DNA replication origins revealed that origins preferentially associate with an Origin G-rich Repeated Element (OGRE), potentially forming G-quadruplexes (G4). Here, we functionally address their requirements for DNA replication initiation in a series of independent approaches. Deletion of the OGRE/G4 sequence strongly decreased the corresponding origin activity. Conversely, the insertion of an OGRE/G4 element created a new replication origin. This element also promoted replication of episomal EBV vectors lacking the viral origin, but not if the OGRE/G4 sequence was deleted. A potent G4 ligand, PhenDC3, stabilized G4s but did not alter the global origin activity. However, a set of new, G4-associated origins was created, whereas suppressed origins were largely G4-free. In vitro Xenopus laevis replication systems showed that OGRE/G4 sequences are involved in the activation of DNA replication, but not in the pre-replication complex formation. Altogether, these results converge to the functional importance of OGRE/G4 elements in DNA replication initiation., Origins of replications are associated with potential G quadruplexes forming structures (G4s). Here the authors reveal the functional role of G4 elements in DNA replication initiation.
- Published
- 2018
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