1. Microglia control vascular architecture via a TGFβ1 dependent paracrine mechanism linked to tissue mechanics.
- Author
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Dudiki T, Meller J, Mahajan G, Liu H, Zhevlakova I, Stefl S, Witherow C, Podrez E, Kothapalli CR, and Byzova TV
- Subjects
- Actomyosin physiology, Animals, Biomechanical Phenomena, Cell Movement physiology, Cytoskeletal Proteins deficiency, Cytoskeletal Proteins genetics, Cytoskeletal Proteins physiology, Female, Hydrogels, Integrins physiology, MAP Kinase Signaling System, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Microglia cytology, Paracrine Communication, Retina growth & development, Retinal Vessels cytology, Retinal Vessels growth & development, Transforming Growth Factor beta1 genetics, Microglia physiology, Retinal Vessels innervation, Transforming Growth Factor beta1 physiology
- Abstract
Tissue microarchitecture and mechanics are important in development and pathologies of the Central Nervous System (CNS); however, their coordinating mechanisms are unclear. Here, we report that during colonization of the retina, microglia contacts the deep layer of high stiffness, which coincides with microglial bipolarization, reduction in TGFβ1 signaling and termination of vascular growth. Likewise, stiff substrates induce microglial bipolarization and diminish TGFβ1 expression in hydrogels. Both microglial bipolarization in vivo and the responses to stiff substrates in vitro require intracellular adaptor Kindlin3 but not microglial integrins. Lack of Kindlin3 causes high microglial contractility, dysregulation of ERK signaling, excessive TGFβ1 expression and abnormally-patterned vasculature with severe malformations in the area of photoreceptors. Both excessive TGFβ1 signaling and vascular defects caused by Kindlin3-deficient microglia are rescued by either microglial depletion or microglial knockout of TGFβ1 in vivo. This mechanism underlies an interplay between microglia, vascular patterning and tissue mechanics within the CNS.
- Published
- 2020
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