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Start Over Author "Shouyue Zhang" Journal nature communications
3 results on '"Shouyue Zhang"'

1. Cas12e orthologs evolve variable structural elements to facilitate dsDNA cleavage

Author

Danyuan Li, Shouyue Zhang, Shuo Lin, Wenjing Xing, Yun Yang, Fengxia Zhu, Dingding Su, Chunlai Chen, and Jun-Jie Gogo Liu

Subjects
Science
Abstract

Abstract Exceptionally diverse type V CRISPR-Cas systems provide numerous RNA-guided nucleases as powerful tools for DNA manipulation. Two known Cas12e nucleases, DpbCas12e and PlmCas12e, are both effective in genome editing. However, many differences exist in their in vitro dsDNA cleavage activities, reflecting the diversity in Cas12e’s enzymatic properties. To comprehensively understand the Cas12e family, we identify and characterize six unreported Cas12e members that vary in their CRISPR-locus architectures, PAM preferences, and cleavage efficacies. Interestingly, among all variants, PlmCas12e exhibits the most robust trans-cleavage activity and the lowest salt sensitivity in cis-cleavage. Further structural comparisons reveal that the unique NTSB domain in PlmCas12e is beneficial to DNA unwinding at high salt concentrations, while some NTSB-lacking Cas12e proteins rely on positively charged loops for dsDNA unwinding. These findings demonstrate how divergent evolution of structural elements shapes the nuclease diversity within the Cas12e family, potentially contributing to their adaptations to varying environmental conditions.

Published
2024
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2. Prognostic significance of frequent CLDN18-ARHGAP26/6 fusion in gastric signet-ring cell cancer

Author

Yang Shu, Weihan Zhang, Qianqian Hou, Linyong Zhao, Shouyue Zhang, Jiankang Zhou, Xiaohai Song, Yan Zhang, Dan Jiang, Xinzu Chen, Peiqi Wang, Xuyang Xia, Fei Liao, Dandan Yin, Xiaolong Chen, Xueyan Zhou, Duyu Zhang, Senlin Yin, Kun Yang, Jianping Liu, Leilei Fu, Lan Zhang, Yuelan Wang, Junlong Zhang, Yunfei An, Hua Cheng, Bin Zheng, Hongye Sun, Yinglan Zhao, Yongsheng Wang, Dan Xie, Liang Ouyang, Ping Wang, Wei Zhang, Meng Qiu, Xianghui Fu, Lunzhi Dai, Gu He, Hanshuo Yang, Wei Cheng, Li Yang, Bo Liu, Weimin Li, Biao Dong, Zongguang Zhou, Yuquan Wei, Yong Peng, Heng Xu, and Jiankun Hu

Subjects
Science
Abstract

Signet-ring cell carcinoma (SRCC) is a unique type of gastric cancer with no prognostic features. Here, the authors report a CLDN18-ARHGAP26/6 gene fusion in patients with a high signet-ring cell content, poor survival outcomes, and who experience no benefit from platinum/fluoropyrimidines-based chemotherapy.

Published
2018
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3. Prognostic significance of frequent CLDN18-ARHGAP26/6 fusion in gastric signet-ring cell cancer

Author

Lunzhi Dai, Yong Peng, Yang Shu, Shouyue Zhang, Gu He, Meng Qiu, Xuyang Xia, Dan Jiang, Ping Wang, Heng Xu, Xueyan Zhou, Peiqi Wang, Xiao-Long Chen, Xiao-Hai Song, Dan Xie, Bin Zheng, Yongsheng Wang, Kun Yang, Biao Dong, Yinglan Zhao, Senlin Yin, Qianqian Hou, Weimin Li, Wei-Han Zhang, Bo Liu, Dandan Yin, Jian-Kang Zhou, Hongye Sun, Fei Liao, Wei Zhang, Hua Cheng, Xin-Zu Chen, Leilei Fu, Junlong Zhang, Yunfei An, Xianghui Fu, Zong-Guang Zhou, Jiankun Hu, Jian-Ping Liu, Lan Zhang, Duyu Zhang, Yan Zhang, Hanshuo Yang, Wei Cheng, Y. Wang, Yuquan Wei, Li Yang, Liang Ouyang, and Lin-Yong Zhao

Subjects
0301 basic medicine, Oncology, Male, medicine.medical_specialty, Science, medicine.medical_treatment, Mutant Chimeric Proteins, General Physics and Astronomy, Antineoplastic Agents, medicine.disease_cause, General Biochemistry, Genetics and Molecular Biology, Article, 03 medical and health sciences, 0302 clinical medicine, Stomach Neoplasms, Internal medicine, Cell Line, Tumor, Carcinoma, medicine, Humans, Stage (cooking), lcsh:Science, Retrospective Studies, Chemotherapy, Multidisciplinary, Whole Genome Sequencing, Signet ring cell, business.industry, GTPase-Activating Proteins, Cancer, Retrospective cohort study, General Chemistry, medicine.disease, digestive system diseases, Oxaliplatin, 030104 developmental biology, Treatment Outcome, Drug Resistance, Neoplasm, 030220 oncology & carcinogenesis, Claudins, lcsh:Q, Female, Carcinogenesis, business, Carcinoma, Signet Ring Cell, medicine.drug
Abstract

Signet-ring cell carcinoma (SRCC) has specific epidemiology and oncogenesis in gastric cancer, however, with no systematical investigation for prognostic genomic features. Here we report a systematic investigation conducted in 1868 Chinese gastric cancer patients indicating that signet-ring cells content was related to multiple clinical characteristics and treatment outcomes. We thus perform whole-genome sequencing on 32 pairs of SRC samples, and identify frequent CLDN18-ARHGAP26/6 fusion (25%). With 797 additional patients for validation, prevalence of CLDN18-ARHGAP26/6 fusion is noticed to be associated with signet-ring cell content, age at diagnosis, female/male ratio, and TNM stage. Importantly, patients with CLDN18-ARHGAP26/6 fusion have worse survival outcomes, and get no benefit from oxaliplatin/fluoropyrimidines-based chemotherapy, which is consistent with the fact of chemo-drug resistance acquired in CLDN18-ARHGAP26 introduced cell lines. Overall, this study provides insights into the clinical and genomic features of SRCC, and highlights the importance of frequent CLDN18-ARHGAP26/6 fusions in chemotherapy response for SRCC., Signet-ring cell carcinoma (SRCC) is a unique type of gastric cancer with no prognostic features. Here, the authors report a CLDN18-ARHGAP26/6 gene fusion in patients with a high signet-ring cell content, poor survival outcomes, and who experience no benefit from platinum/fluoropyrimidines-based chemotherapy.

Published
2018

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