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Your search keyword '"Bendelac, Albert"' showing total 14 results
Start Over Author "Bendelac, Albert" Journal nature immunology
14 results on '"Bendelac, Albert"'

2. Single cell analysis defines the divergence between the innate lymphoid cell and lymphoid tissue inducer lineages

Author

Ishizuka, Isabel E., Chea, Sylvestre, Gudjonson, Herman, Constantinides, Michael G., Dinner, Aaron R., Bendelac, Albert, and Golub, Rachel

Subjects
Lymphoid Tissue, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, Kruppel-Like Transcription Factors, Cell Differentiation, T-Lymphocytes, Helper-Inducer, Flow Cytometry, Article, Lymphocyte Subsets, Mice, Animals, Cell Lineage, Promyelocytic Leukemia Zinc Finger Protein, Hepatocyte Nuclear Factor 1-alpha, Lymphocytes, Single-Cell Analysis, Multiplex Polymerase Chain Reaction
Abstract

The precise lineage relationship between innate lymphoid cells (ILCs) and lymphoid tissue-inducer (LTi) cells is poorly understood. Using single-cell multiplex transcriptional analysis of 100 lymphoid genes and single-cell cultures of fetal liver precursor cells, we identified the common proximal precursor to these lineages and found that its bifurcation was marked by differential induction of the transcription factors PLZF and TCF1. Acquisition of individual effector programs specific to the ILC subsets ILC1, ILC2 and ILC3 was initiated later, at the common ILC precursor stage, by transient expression of mixed ILC1, ILC2 and ILC3 transcriptional patterns, whereas, in contrast, the development of LTi cells did not go through multilineage priming. Our findings provide insight into the divergent mechanisms of the differentiation of the ILC lineage and LTi cell lineage and establish a high-resolution 'blueprint' of their development.

Published
2016

12. Multiple defects in antigen presentation and T cell development by mice expressing cytoplasmic tail?truncated CD1d.

Author

Chiu, Ya-Hui, Park, Se-Ho, Benlagha, Kamel, Forestier, Claire, Jayawardena-Wolf, Jayanthi, Savage, Paul B., Teyton, Luc, and Bendelac, Albert

Subjects
TYROSINE, ANTIGENS
Abstract

For members of the CD1 family of β2-microglobulin?associated lipid-presenting molecules, tyrosine-based motifs in the cytoplasmic tail and invariant chain (Ii) govern glycoprotein trafficking through endosomal compartments. Little is known about the intracellular pathways of CD1 trafficking and antigen presentation. However, in vitro studies with cells transfected with mutant CD1 that had a truncated cytoplasmic tail have suggested a role for these tyrosine motifs in some, but not all, antigenic systems. By introducing a deletion of the tyrosine motif into the germ line, and through homologous recombination in embryonic stem cells, we now describe knock-in mice with the CD1d cytoplasmic tail deleted. Despite adequate surface CD1d expression and the presence of Ii, these mutant mice showed multiple and selective abnormalities in intracellular trafficking, antigen presentation and T cell development, demonstrating the critical functions of the CD1d cytoplasmic tail motif in vivo. [ABSTRACT FROM AUTHOR]

Published
2002
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14. Multiple defects in antigen presentation and T cell development by mice expressing cytoplasmic tail-truncated CD1d.

Author

Chiu YH, Park SH, Benlagha K, Forestier C, Jayawardena-Wolf J, Savage PB, Teyton L, and Bendelac A

Subjects
Amino Acid Motifs, Animals, Antigens immunology, Antigens, CD1 chemistry, Antigens, CD1 genetics, Antigens, CD1d, B-Lymphocytes immunology, Biological Transport, Biotinylation, Clonal Deletion, Crosses, Genetic, Dendritic Cells immunology, Endosomes metabolism, Female, Galactosylceramides immunology, Hybridomas immunology, Macrophages immunology, Male, Mice, Mice, Inbred C57BL, Protein Transport, Receptors, Antigen, T-Cell, alpha-beta immunology, Recombinant Fusion Proteins chemistry, Recombinant Fusion Proteins immunology, Sequence Deletion, Specific Pathogen-Free Organisms, Stem Cells, Thymus Gland immunology, Transfection, Antigen Presentation physiology, Antigens, CD1 immunology, Gene Targeting, Phosphotyrosine physiology, T-Lymphocyte Subsets immunology, Thymus Gland cytology
Abstract

For members of the CD1 family of beta(2)-microglobulin-associated lipid-presenting molecules, tyrosine-based motifs in the cytoplasmic tail and invariant chain (Ii) govern glycoprotein trafficking through endosomal compartments. Little is known about the intracellular pathways of CD1 trafficking and antigen presentation. However, in vitro studies with cells transfected with mutant CD1 that had a truncated cytoplasmic tail have suggested a role for these tyrosine motifs in some, but not all, antigenic systems. By introducing a deletion of the tyrosine motif into the germ line, and through homologous recombination in embryonic stem cells, we now describe knock-in mice with the CD1d cytoplasmic tail deleted. Despite adequate surface CD1d expression and the presence of Ii, these mutant mice showed multiple and selective abnormalities in intracellular trafficking, antigen presentation and T cell development, demonstrating the critical functions of the CD1d cytoplasmic tail motif in vivo.

Published
2002
Full Text
View/download PDF

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