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Your search keyword '"Shaw, Andrey S"' showing total 12 results
Start Over Author "Shaw, Andrey S" Journal nature immunology
12 results on '"Shaw, Andrey S"'

1. Gremlin 1+ fibroblastic niche maintains dendritic cell homeostasis in lymphoid tissues

Author

Kapoor, Varun N., Müller, Sören, Keerthivasan, Shilpa, Brown, Markus, Chalouni, Cecile, Storm, Elaine E., Castiglioni, Alessandra, Lane, Ryan, Nitschke, Maximilian, Dominguez, Claudia X., Astarita, Jillian L., Krishnamurty, Akshay T., Carbone, Catherine B., Senbabaoglu, Yasin, Wang, Amber W., Wu, Xiumin, Cremasco, Viviana, Roose-Girma, Merone, Tam, Lucinda, Doerr, Jonas, Chen, Mark Z., Lee, Wyne P., Modrusan, Zora, Yang, Yeqing Angela, Bourgon, Richard, Sandoval, Wendy, Shaw, Andrey S., de Sauvage, Frederic J., Mellman, Ira, Moussion, Christine, and Turley, Shannon J.

Published
2021
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3. Gremlin 1+fibroblastic niche maintains dendritic cell homeostasis in lymphoid tissues

Author

Kapoor, Varun N., Müller, Sören, Keerthivasan, Shilpa, Brown, Markus, Chalouni, Cecile, Storm, Elaine E., Castiglioni, Alessandra, Lane, Ryan, Nitschke, Maximilian, Dominguez, Claudia X., Astarita, Jillian L., Krishnamurty, Akshay T., Carbone, Catherine B., Senbabaoglu, Yasin, Wang, Amber W., Wu, Xiumin, Cremasco, Viviana, Roose-Girma, Merone, Tam, Lucinda, Doerr, Jonas, Chen, Mark Z., Lee, Wyne P., Modrusan, Zora, Yang, Yeqing Angela, Bourgon, Richard, Sandoval, Wendy, Shaw, Andrey S., de Sauvage, Frederic J., Mellman, Ira, Moussion, Christine, and Turley, Shannon J.

Abstract

Fibroblastic reticular cells (FRCs) are specialized stromal cells that define tissue architecture and regulate lymphocyte compartmentalization, homeostasis, and innate and adaptive immunity in secondary lymphoid organs (SLOs). In the present study, we used single-cell RNA sequencing (scRNA-seq) of human and mouse lymph nodes (LNs) to identify a subset of T cell–zone FRCs defined by the expression of Gremlin1 (Grem1) in both species. Grem1-CreERT2knock-in mice enabled localization, multi-omics characterization and genetic depletion of Grem1+FRCs. Grem1+FRCs primarily localize at T–B cell junctions of SLOs, neighboring pre-dendritic cells and conventional dendritic cells (cDCs). As such, their depletion resulted in preferential loss and decreased homeostatic proliferation and survival of resident cDCs and compromised T cell immunity. Trajectory analysis of human LN scRNA-seq data revealed expression similarities to murine FRCs, with GREM1+cells marking the endpoint of both trajectories. These findings illuminate a new Grem1+fibroblastic niche in LNs that functions to maintain the homeostasis of lymphoid tissue-resident cDCs.

Published
2021
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4. Macrophage colony-stimulating factor induces the proliferation and survival of macrophages via a pathway involving DAP12 and β-catenin

Author

Otero, Karel, primary, Turnbull, Isaiah R, additional, Poliani, Pietro Luigi, additional, Vermi, William, additional, Cerutti, Elisa, additional, Aoshi, Taiki, additional, Tassi, Ilaria, additional, Takai, Toshiyuki, additional, Stanley, Samuel L, additional, Miller, Mark, additional, Shaw, Andrey S, additional, and Colonna, Marco, additional

Published
2009
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10. The immunological synapse and CD28-CD80 interactions

Author

Bromley, Shannon K., primary, Iaboni, Andrea, additional, Davis, Simon J., additional, Whitty, Adrian, additional, Green, Jonathan M., additional, Shaw, Andrey S., additional, Weiss, Arthur, additional, and Dustin, Michael L., additional

Published
2001
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12. Macrophage colony-stimulating factor induces the proliferation and survival of macrophages via a pathway involving DAP12 and beta-catenin.

Author

Otero K, Turnbull IR, Poliani PL, Vermi W, Cerutti E, Aoshi T, Tassi I, Takai T, Stanley SL, Miller M, Shaw AS, and Colonna M

Subjects
Adaptor Proteins, Signal Transducing genetics, Animals, Brain metabolism, Brain pathology, Calcium-Binding Proteins metabolism, Cell Adhesion drug effects, Cell Cycle drug effects, Cell Survival drug effects, Cells, Cultured, Flow Cytometry, Focal Adhesion Kinase 2 metabolism, Green Fluorescent Proteins genetics, Green Fluorescent Proteins metabolism, Immunoblotting, Immunohistochemistry, Macrophages cytology, Macrophages metabolism, Mice, Mice, Inbred C57BL, Mice, Inbred Strains, Mice, Knockout, Mice, Transgenic, Microfilament Proteins, Phosphorylation, Adaptor Proteins, Signal Transducing metabolism, Cell Proliferation drug effects, Macrophage Colony-Stimulating Factor pharmacology, Macrophages drug effects, Signal Transduction drug effects, beta Catenin metabolism
Abstract

Macrophage colony-stimulating factor (M-CSF) influences the proliferation and survival of mononuclear phagocytes through the receptor CSF-1R. The adaptor protein DAP12 is critical for the function of mononuclear phagocytes. DAP12-mutant mice and humans have defects in osteoclasts and microglia, as well as brain and bone abnormalities. Here we show DAP12 deficiency impaired the M-CSF-induced proliferation and survival of macrophages in vitro. DAP12-deficient mice had fewer microglia in defined central nervous system areas, and DAP12-deficient progenitors regenerated myeloid cells inefficiently after bone marrow transplantation. Signaling by M-CSF through CSF-1R induced the stabilization and nuclear translocation of beta-catenin, which activated genes involved in the cell cycle. DAP12 was essential for phosphorylation and nuclear accumulation of beta-catenin. Our results provide a mechanistic explanation for the many defects of DAP12-deficient mononuclear phagocytes.

Published
2009
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