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Deep profiling of antibody and T cell-receptor repertoires by means of high-throughput sequencing has become an attractive approach for adaptive immunity studies, but its power is substantially compromised by the accumulation of PCR and sequencing errors. Here we report MIGEC (molecular identifier groups-based error correction), a strategy for high-throughput sequencing data analysis. MIGEC allows for nearly absolute error correction while fully preserving the natural diversity of complex immune repertoires. [ABSTRACT FROM AUTHOR]

Fluorescent proteins with emission wavelengths in the near-infrared and infrared range are in high demand for whole-body imaging techniques. Here we report near-infrared dimeric fluorescent proteins eqFP650 and eqFP670. To our knowledge, eqFP650 is the brightest fluorescent protein with emission maximum above 635 nm, and eqFP670 displays the most red-shifted emission maximum and high photostability. [ABSTRACT FROM AUTHOR]

The article presents information on the MiTCR, a software package, which extracts information of T-cell antigen receptor (TCR) from sequencing data. It mentions that MiTCR carries out CDR3 extraction, recognizes V, D and J segments and gathers clonotypes. It also informs that more than 80 tests units have evaluated MiTCR modules, thereby improving the reliability and correctness of the code.