1. Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II
- Author
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Sierra, Raymond G, Gati, Cornelius, Laksmono, Hartawan, Dao, E Han, Gul, Sheraz, Fuller, Franklin, Kern, Jan, Chatterjee, Ruchira, Ibrahim, Mohamed, Brewster, Aaron S, Young, Iris D, Michels-Clark, Tara, Aquila, Andrew, Liang, Mengning, Hunter, Mark S, Koglin, Jason E, Boutet, Sébastien, Junco, Elia A, Hayes, Brandon, Bogan, Michael J, Hampton, Christina Y, Puglisi, Elisabetta V, Sauter, Nicholas K, Stan, Claudiu A, Zouni, Athina, Yano, Junko, Yachandra, Vittal K, Soltis, S Michael, Puglisi, Joseph D, and DeMirci, Hasan
- Subjects
Biological Sciences ,Crystallography ,Models ,Molecular ,Photosystem II Protein Complex ,Ribosomes ,Technology ,Medical and Health Sciences ,Developmental Biology ,Biological sciences - Abstract
We describe a concentric-flow electrokinetic injector for efficiently delivering microcrystals for serial femtosecond X-ray crystallography analysis that enables studies of challenging biological systems in their unadulterated mother liquor. We used the injector to analyze microcrystals of Geobacillus stearothermophilus thermolysin (2.2-Å structure), Thermosynechococcus elongatus photosystem II (
- Published
- 2016