1. Imaging-based screens of pool-synthesized cell libraries
- Author
-
Michael J. Lawson and Johan Elf
- Subjects
0303 health sciences ,2019-20 coronavirus outbreak ,Coronavirus disease 2019 (COVID-19) ,Computer science ,Strain (biology) ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Cell ,Cell Biology ,Computational biology ,Models, Biological ,Biochemistry ,Cell Line ,High-Throughput Screening Assays ,Open Reading Frames ,03 medical and health sciences ,Phenotype ,medicine.anatomical_structure ,Mutation ,medicine ,Humans ,Molecular Biology ,030304 developmental biology ,Biotechnology - Abstract
Mapping a genetic perturbation to a change in phenotype is at the core of biological research. Advances in microscopy have transformed these studies, but they have largely been confined to examining a few strains or cell lines at a time. In parallel, there has been a revolution in creating synthetic libraries of genetically altered cells with relative ease. Here we describe methods that combine these powerful tools to perform live-cell imaging of pool-generated strain libraries for improved biological discovery.
- Published
- 2021