1. BigStitcher: reconstructing high-resolution image datasets of cleared and expanded samples.
- Author
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Hörl D, Rojas Rusak F, Preusser F, Tillberg P, Randel N, Chhetri RK, Cardona A, Keller PJ, Harz H, Leonhardt H, Treier M, and Preibisch S
- Subjects
- Animals, Caenorhabditis elegans, Drosophila, Female, Imaging, Three-Dimensional methods, Mice, Brain diagnostic imaging, Brain metabolism, Green Fluorescent Proteins metabolism, Image Processing, Computer-Assisted methods, Microscopy, Fluorescence methods, Software
- Abstract
Light-sheet imaging of cleared and expanded samples creates terabyte-sized datasets that consist of many unaligned three-dimensional image tiles, which must be reconstructed before analysis. We developed the BigStitcher software to address this challenge. BigStitcher enables interactive visualization, fast and precise alignment, spatially resolved quality estimation, real-time fusion and deconvolution of dual-illumination, multitile, multiview datasets. The software also compensates for optical effects, thereby improving accuracy and enabling subsequent biological analysis.
- Published
- 2019
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