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Entanglement is the central yet fleeting phenomenon of quantum physics. Once being considered a peculiar counter-intuitive property of quantum theory, it has developed into the most central element of quantum technology. Consequently, there have been a number of experimental demonstrations of entanglement between photons, atoms, ions and solid-state systems such as spins or quantum dots, superconducting circuits and macroscopic diamond. Here we experimentally demonstrate entanglement between two engineered single solid-state spin quantum bits (qubits) at ambient conditions. Photon emission of defect pairs reveals ground-state spin correlation. Entanglement (fidelity = 0.67±0.04) is proved by quantum state tomography. Moreover, the lifetime of electron spin entanglement is extended to milliseconds by entanglement swapping to nuclear spins. The experiments mark an important step towards a scalable room-temperature quantum device being of potential use in quantum information processing as well as metrology. [ABSTRACT FROM AUTHOR]