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Summary: A new aphelenchoidid entomoparasitic nematode was isolated from the body cavity of overwintering individuals of a tenebrionid beetle, Uloma marseuli , collected at Shiga, Japan. The nematode is characterised by its six equal-sized lips forming a dome shape continuous with body contour. It has a moderately thick stylet, with wide lumen, a long two-part long (conus + conophore) and elongate oval or pear-shaped metacorpus with glandular anterior part. The male spicules are separate with a well-developed condylus, triangular rostrum and smoothly and strongly curved calomus-lamina complex. A gubernaculum or apophysis is absent. There are two pairs of papilliform male genital papillae. Females lack a post-vulval uterine sac, have a seemingly vestigial rectum and anus, and conical tail. The combination of the typological characters of the species does not fit any currently accepted aphelenchoidid genus and is somewhat intermediate between the Ektaphelenchinae and Entaphelenchinae. The molecular phylogenetic analysis also suggested that the nematode is close to both Ektaphelenchoides (Ektaphelenchinae) and Peraphelenchus (Entaphelenchinae). Thus, the nematode is described and illustrated as Lenisaphelenchus ulomae n. gen., n. sp. and tentatively placed in the Ektaphelenchinae. [ABSTRACT FROM AUTHOR]

Summary: Bursaphelenchus acaloleptae n. sp. is described and figured based upon its morphological characters and molecular profiles. The new species belongs to the xylophilus -group of the genus and is typologically characterised by its secretory-excretory pore being located slightly posterior to the median bulb, weakly tapering, relatively broad and straight female tail with variously shaped terminus, and rounded male bursal flap with squared terminus. The new species is phylogenetically and biologically closest to B. luxuriosae. These cryptic species share the host (habitat) tree species, Aralia elata (Araliaceae), the same carrier insect species, Acalolepta luxuriosa (Cerambycidae), and a characteristic phoretic adult form. However, the new species is typologically distinguished from all other xylophilus -group species by the combination of the above-mentioned characters and molecular profiles (SSU and LSU molecular barcodes). Further, B. acaloleptae n. sp. can be distinguished from its closest relative, B. luxuriosae , by mating experiments, i.e. , these two species did not produce a viable F1 generation in reciprocal crossings, thereby confirming separate species status using the biological species concept. [ABSTRACT FROM AUTHOR]