1. Activation of adenosine A 2A receptors in the olfactory tubercle promotes sleep in rodents.
- Author
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Li R, Wang YQ, Liu WY, Zhang MQ, Li L, Cherasse Y, Schiffmann SN, de Kerchove d'Exaerde A, Lazarus M, Qu WM, and Huang ZL
- Subjects
- Adenosine analogs & derivatives, Adenosine pharmacology, Animals, Electroencephalography methods, Male, Mice, Mice, Inbred C57BL, Mice, Transgenic, Olfactory Tubercle drug effects, Phenethylamines pharmacology, Rats, Rats, Sprague-Dawley, Receptor, Adenosine A2A genetics, Rodentia, Sleep drug effects, Adenosine A2 Receptor Agonists pharmacology, Olfactory Tubercle metabolism, Receptor, Adenosine A2A metabolism, Sleep physiology
- Abstract
The olfactory tubercle (OT), an important nucleus in processing sensory information, has been reported to change cortical activity under odor. However, little is known about the physiological role and mechanism of the OT in sleep-wake regulation. The OT expresses abundant adenosine A
2A receptors (A2A Rs), which are important in sleep regulation. Therefore, we hypothesized that the OT regulates sleep via A2A Rs. This study examined sleep-wake profiles through electroencephalography and electromyography recordings with pharmacological and chemogenetic manipulations in freely moving rodents. Compared with their controls, activation of OT A2A Rs pharmacologically and OT A2A R neurons via chemogenetics increased non-rapid eye movement sleep for 5 and 3 h, respectively, while blockade of A2A Rs decreased non-rapid eye movement sleep. Tracing and electrophysiological studies showed OT A2A R neurons projected to the ventral pallidum and lateral hypothalamus, forming inhibitory innervations. Together, these findings indicate that A2A Rs in the OT play an important role in sleep regulation., Competing Interests: Declaration of competing interest The authors declare no competing interests., (Copyright © 2019. Published by Elsevier Ltd.)- Published
- 2020
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