12 results on '"Marisa Roberto"'
Search Results
2. Decreased excitability of leptin-sensitive anterior insula pyramidal neurons in a rat model of compulsive food demand
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Dean Kirson, Samantha R. Spierling Bagsic, Jiayuan Murphy, Hang Chang, Roman Vlkolinsky, Sarah N. Pucci, Julia Prinzi, Casey A. Williams, Savannah Y. Fang, Marisa Roberto, and Eric P. Zorrilla
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Leptin ,Pharmacology ,Cellular and Molecular Neuroscience ,Pyramidal Cells ,Compulsive Behavior ,Animals ,Humans ,Female ,Feeding Behavior ,Rats, Wistar ,Article ,Rats - Abstract
Compulsive eating is an overlapping construct with binge eating and shares many characteristics with substance abuse disorders. Compulsive eating may impact millions of Americans; presenting in some cases of binge eating disorders, overweight/obesity, and among individuals who have not yet been diagnosed with a recognized eating disorder. To study the behavioral and neurobiological underpinnings of compulsive eating, we employ a published rodent model using cyclic intermittent access to a palatable diet to develop a self-imposed binge-withdrawal cycle. Here, we further validated this model of compulsive eating in female Wistar rats, through the lens of behavioral economic analyses, and observed heightened demand intensity, inelasticity and essential value as well as increased food-seeking during extinction. Using electrophysiological recordings in the anterior insular cortex, a region previously implicated in modulating compulsive-like eating in intermittent access models, we observed functional adaptations of pyramidal neurons. Within the same neurons, application of leptin led to further functional adaptations, suggesting a previously understudied, extrahypothalamic role of leptin in modulating feeding-related cortical circuits. Collectively, the findings suggest that leptin may modulate food-related motivation or decisionmaking via a plastic cortical circuit that is influenced by intermitted access to a preferred diet. These findings warrant further study for whether the behavioral economics of compulsive eating behavior can impact disordered eating outcomes in humans, and whether there is translational relevance of a leptin-sensitive anterior insular circuit implicated in these behaviors.
- Published
- 2022
3. Morphological and functional evidence of increased excitatory signaling in the prelimbic cortex during ethanol withdrawal
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Marisa Roberto, Florence P. Varodayan, Max Kreifeldt, Harpreet Sidhu, and Candice Contet
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Male ,0301 basic medicine ,Patch-Clamp Techniques ,Dendritic spine ,Dendritic Spines ,Infralimbic cortex ,Prefrontal Cortex ,In Vitro Techniques ,Biology ,Neurotransmission ,Article ,Mice ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,Glutamatergic ,0302 clinical medicine ,medicine ,Animals ,Maze Learning ,Prefrontal cortex ,Pharmacology ,Analysis of Variance ,Ethanol ,Pyramidal Cells ,Glutamate receptor ,Central Nervous System Depressants ,Synaptic Potentials ,Substance Withdrawal Syndrome ,Barnes maze ,Mice, Inbred C57BL ,Alcoholism ,030104 developmental biology ,medicine.anatomical_structure ,Excitatory postsynaptic potential ,Neuroscience ,030217 neurology & neurosurgery ,Signal Transduction - Abstract
Excessive alcohol consumption in humans induces deficits in decision making and emotional processing, which indicates a dysfunction of the prefrontal cortex (PFC). The present study aimed to determine the impact of chronic intermittent ethanol (CIE) inhalation on mouse medial PFC pyramidal neurons. Data were collected 6-8 days into withdrawal from 7 weeks of CIE exposure, a time point when mice exhibit behavioral symptoms of withdrawal. We found that spine maturity in prelimbic (PL) layer 2/3 neurons was increased, while dendritic spines in PL layer 5 neurons or infralimbic (IL) neurons were not affected. Corroborating these morphological observations, CIE enhanced glutamatergic transmission in PL layer 2/3 pyramidal neurons, but not IL layer 2/3 neurons. Contrary to our predictions, these cellular alterations were associated with improved, rather than impaired, performance in reversal learning and strategy switching tasks in the Barnes maze at an earlier stage of chronic ethanol exposure (5-7 days withdrawal from 3 to 4 weeks of CIE), which could result from the anxiety-like behavior associated with ethanol withdrawal. Altogether, this study adds to a growing body of literature indicating that glutamatergic activity in the PFC is upregulated following chronic ethanol exposure, and identifies PL layer 2/3 pyramidal neurons as a sensitive target of synaptic remodeling. It also indicates that the Barnes maze is not suitable to detect deficits in cognitive flexibility in CIE-withdrawn mice.
- Published
- 2018
4. PACAP regulation of central amygdala GABAergic synapses is altered by restraint stress
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M.W. Riley, Florence P. Varodayan, Christopher S. Oleata, Valentina Sabino, Marisa Roberto, Margaret A. Minnig, and Michael Q. Steinman
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0301 basic medicine ,Male ,Restraint, Physical ,Neuropeptide ,Biology ,Neurotransmission ,Amygdala ,Article ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,0302 clinical medicine ,medicine ,Premovement neuronal activity ,Animals ,GABAergic Neurons ,Rats, Wistar ,Receptor ,gamma-Aminobutyric Acid ,Pharmacology ,Central Amygdaloid Nucleus ,Rats ,Electrophysiology ,030104 developmental biology ,medicine.anatomical_structure ,Anxiogenic ,Inhibitory Postsynaptic Potentials ,Synapses ,GABAergic ,Pituitary Adenylate Cyclase-Activating Polypeptide ,Neuroscience ,030217 neurology & neurosurgery ,Stress, Psychological ,Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I - Abstract
The pituitary adenylate cyclase-activating polypeptide (PACAP) system plays a central role in the brain's emotional response to psychological stress by activating cellular processes and circuits associated with threat exposure. The neuropeptide PACAP and its main receptor PAC1 are expressed in the rodent central amygdala (CeA), a brain region critical in negative emotional processing, and CeA PACAPergic signaling drives anxiogenic and stress coping behaviors. Despite this behavioral evidence, PACAP's effects on neuronal activity within the medial subdivision of the CeA (CeM, the major output nucleus for the entire amygdala complex) during basal conditions and after psychological stress remain unknown. Therefore, in the present study, male Wistar rats were subjected to either restraint stress or control conditions, and PACAPergic regulation of CeM cellular function was assessed using immunohistochemistry and whole-cell patch-clamp electrophysiology. Our results demonstrate that PACAP-38 potentiates GABA release in the CeM of naive rats, via its actions at presynaptic PAC1. Basal PAC1 activity also enhances GABA release in an action potential-dependent manner. Notably, PACAP-38's facilitation of CeM GABA release was attenuated after a single restraint stress session, but after repeated sessions returned to the level observed in naive animals. A single restraint session also significantly decreased PAC1 levels in the CeM, with repeated restraint sessions producing a slight recovery. Collectively our data reveal that PACAP/PAC1 signaling enhances inhibitory control of the CeM and that psychological stress can modulate this influence to potentially disinhibit downstream effector regions that mediate anxiety and stress-related behaviors. This article is part of the special issue on 'Neuropeptides'.
- Published
- 2019
5. Glutamatergic transmission in the central nucleus of the amygdala is selectively altered in Marchigian Sardinian alcohol-preferring rats: Alcohol and CRF effects
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Roberto Ciccocioppo, Dean Kirson, Florence P. Varodayan, Melissa A. Herman, Marisa Roberto, George Luu, Markus Heilig, and Christopher S. Oleata
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0301 basic medicine ,medicine.medical_specialty ,Alcohol Drinking ,Glutamic Acid ,Biology ,Receptors, Corticotropin-Releasing Hormone ,Amygdala ,Article ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,Glutamatergic ,0302 clinical medicine ,Internal medicine ,medicine ,Animals ,Rats, Wistar ,neoplasms ,Neurons ,Pharmacology ,Ethanol ,Central nucleus of the amygdala ,Central Amygdaloid Nucleus ,Glutamate receptor ,Antagonist ,Excitatory Postsynaptic Potentials ,Glutamic acid ,Rats ,Electrophysiology ,Pyrimidines ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,Biochemistry ,Excitatory postsynaptic potential ,030217 neurology & neurosurgery - Abstract
The CRF system of the central nucleus of the amygdala (CeA) is important for the processing of anxiety, stress, and effects of acute and chronic ethanol. We previously reported that ethanol decreases evoked glutamate transmission in the CeA of Sprague Dawley rats and that ethanol dependence alters glutamate release in the CeA. Here, we examined the effects of ethanol, CRF and a CRF1 receptor antagonist on spontaneous and evoked glutamatergic transmission in CeA neurons from Wistar and Marchigian Sardinian Preferring (msP) rats, a rodent line genetically selected for excessive alcohol drinking and characterized by heightened activity of the CRF1 system. Basal spontaneous and evoked glutamate transmission in CeA neurons from msP rats was increased compared to Wistar rats. Ethanol had divergent effects, either increasing or decreasing spontaneous glutamate release in the CeA of Wistar rats. This bidirectional effect was retained in msP rats, but the magnitude of the ethanol-induced increase in glutamate release was significantly smaller. The inhibitory effect of ethanol on evoked glutamatergic transmission was similar in both strains. CRF also either increased or decreased spontaneous glutamate release in CeA neurons of Wistar rats, however, in msP rats CRF only increased glutamate release. The inhibitory effect of CRF on evoked glutamatergic transmission was also lost in neurons from msP rats. A CRF1 antagonist produced only minor effects on spontaneous glutamate transmission, which were consistent across strains, and no effects on evoked glutamate transmission. These results demonstrate that the genetically altered CRF system of msP rats results in alterations in spontaneous and stimulated glutamate signaling in the CeA that may contribute to both the anxiety and drinking behavioral phenotypes.
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- 2016
6. CRF modulates glutamate transmission in the central amygdala of naïve and ethanol-dependent rats
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Florence P. Varodayan, George Luu, Christopher S. Oleata, Dean Kirson, Diego Correia, Paul Schweitzer, Sophia Khom, and Marisa Roberto
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0301 basic medicine ,Male ,medicine.medical_specialty ,Corticotropin-Releasing Hormone ,Glutamic Acid ,Biology ,Neurotransmission ,Receptors, Corticotropin-Releasing Hormone ,Synaptic Transmission ,Article ,Rats, Sprague-Dawley ,Tissue Culture Techniques ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,Glutamatergic ,chemistry.chemical_compound ,0302 clinical medicine ,Astressin-B ,Internal medicine ,medicine ,DNQX ,Animals ,Pharmacology ,Neurotransmitter Agents ,Ethanol ,Central nucleus of the amygdala ,Central Amygdaloid Nucleus ,Antagonist ,Glutamate receptor ,Central Nervous System Depressants ,Alcoholism ,Disease Models, Animal ,030104 developmental biology ,Endocrinology ,chemistry ,Synapses ,Excitatory postsynaptic potential ,030217 neurology & neurosurgery ,hormones, hormone substitutes, and hormone antagonists - Abstract
Corticotropin-releasing factor (CRF) signaling in the central nucleus of the amygdala (CeA) is hypothesized to drive the development of alcohol dependence, as it regulates ethanol intake and several anxiogenic behaviors linked to withdrawal. Excitatory glutamatergic neurotransmission contributes to alcohol reinforcement, tolerance and dependence. Therefore, in this study we used in vitro slice electrophysiology to investigate the effects of CRF and its receptor subtype (CRF1 and CRF2) antagonists on both evoked and spontaneous action potential-independent glutamatergic transmission in the CeA of naive and ethanol-dependent Sprague-Dawley rats. We found that CRF (25–200 nM) concentration-dependently diminished evoked compound excitatory postsynaptic potentials (EPSPs), but increased miniature excitatory postsynaptic current (mEPSC) frequencies similarly in CeA neurons of both naive and ethanol-dependent rats, indicating reduced evoked glutamatergic responses and enhanced vesicular glutamate release, respectively. This CRF-induced vesicular glutamate release was prevented by the CRF1/2 antagonist (Astressin B) and the CRF1 antagonist (R121919), but not by the CRF2 antagonist (Astressin 2B). Similarly, CRF's effects on evoked glutamatergic responses were completely blocked by CRF1 antagonism, but only slightly decreased in the presence of the CRF2 antagonist. Moreover, CRF1 antagonism reveals a tonic facilitation of vesicular glutamate, whereas the CRF2 antagonism revealed a tonic inhibition of vesicular glutamate release. Collectively our data show that CRF primarily acts at presynaptic CRF1 to produce opposite effects on CeA evoked and spontaneous glutamate release and that the CRF system modulates CeA glutamatergic synapses throughout the development of alcohol dependence.
- Published
- 2017
7. Dopamine D2 receptor desensitization by dopamine or corticotropin releasing factor in ventral tegmental area neurons is associated with increased glutamate release
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Sudarat Nimitvilai, Maureen A. McElvain, Devinder Arora, Chang You, Marisa Roberto, Melissa A. Herman, and Mark S. Brodie
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Male ,Agonist ,endocrine system ,medicine.medical_specialty ,Patch-Clamp Techniques ,Quinpirole ,Corticotropin-Releasing Hormone ,medicine.drug_class ,Dopamine ,Action Potentials ,Glutamic Acid ,Article ,Cellular and Molecular Neuroscience ,Dopamine receptor D2 ,Internal medicine ,medicine ,Animals ,Rats, Wistar ,Urocortins ,Neurons ,Pharmacology ,Urocortin ,Receptors, Dopamine D2 ,Receptors, Dopamine D1 ,Ventral Tegmental Area ,Dopaminergic ,Glutamate receptor ,Excitatory Postsynaptic Potentials ,Rats, Inbred F344 ,Ventral tegmental area ,Endocrinology ,medicine.anatomical_structure ,Receptors, Glutamate ,nervous system ,Dopamine Agonists ,Excitatory Amino Acid Antagonists ,Central Nervous System Agents ,medicine.drug - Abstract
Neurons of the ventral tegmental area (VTA) are the source of dopaminergic (DAergic) input to important brain regions related to addiction. Prolonged exposure of these VTA neurons to moderate concentrations of dopamine (DA) causes a time-dependent decrease in DA-induced inhibition, a complex desensitization called DA inhibition reversal (DIR). DIR is mediated by conventional protein kinase C (cPKC) through concurrent stimulation of D2 and D1-like DA receptors, or by D2 stimulation concurrent with activation of some Gq-linked receptors. Corticotropin releasing factor (CRF) acts via Gq, and can modulate glutamatergic neurotransmission in the VTA. In the present study, we used brain slice electrophysiology to characterize the interaction of DA, glutamate antagonists, and CRF agonists in the induction and maintenance of DIR in the VTA. Glutamate receptor antagonists blocked induction but not maintenance of DIR. Putative blockers of neurotransmitter release and store-operated calcium channels blocked and reversed DIR. CRF and the CRF agonist urocortin reversed inhibition produced by the D2 agonist quinpirole, consistent with our earlier work indicating that Gq activation reverses quinpirole-mediated inhibition. In whole cell recordings, the combination of urocortin and quinpirole, but not either agent alone, increased spontaneous excitatory postsynaptic currents (sEPSCs) in VTA neurons. Likewise, the combination of a D1-like receptor agonist and quinpirole, but not either agent alone, increased sEPSCs in VTA neurons. In summary, desensitization of D2 receptors induced by dopamine or CRF on DAergic VTA neurons is associated with increased glutamatergic signaling in the VTA.
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- 2014
8. Kappa opioid receptor activation decreases inhibitory transmission and antagonizes alcohol effects in rat central amygdala
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George F. Koob, Paul Schweitzer, Nicholas W. Gilpin, and Marisa Roberto
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Male ,medicine.medical_specialty ,Patch-Clamp Techniques ,Pyrrolidines ,Narcotic Antagonists ,Benzeneacetamides ,Dynorphin ,Pharmacology ,Neurotransmission ,Inhibitory postsynaptic potential ,Dynorphins ,κ-opioid receptor ,Article ,Naltrexone ,Rats, Sprague-Dawley ,Cellular and Molecular Neuroscience ,Postsynaptic potential ,Internal medicine ,medicine ,Animals ,Ethanol ,GABAA receptor ,Chemistry ,Receptors, Opioid, kappa ,Amygdala ,Rats ,Endocrinology ,Inhibitory Postsynaptic Potentials ,nervous system ,Norbinaltorphimine ,medicine.drug - Abstract
Activation of the kappa opioid receptor (KOR) system mediates negative emotional states and considerable evidence suggests that KOR and their natural ligand, dynorphin, are involved in ethanol dependence and reward. The central amygdala (CeA) plays a major role in alcohol dependence and reinforcement. Dynorphin peptide and gene expression are activated in the amygdala during acute and chronic administration of alcohol, but the effects of activation or blockade of KOR on inhibitory transmission and ethanol effects have not been studied. We used the slice preparation to investigate the physiological role of KOR and interaction with ethanol on GABAA receptor-mediated synaptic transmission. Superfusion of dynorphin or U69593 onto CeA neurons decreased evoked inhibitory postsynaptic potentials (IPSPs) in a concentration-dependent manner, an effect prevented by the KOR antagonist norbinaltorphimine (norBNI). Applied alone, norBNI increased GABAergic transmission, revealing a tonic endogenous activity at KOR. Paired-pulse analysis suggested a presynaptic KOR mechanism. Superfusion of ethanol increased IPSPs and pretreatment with KOR agonists diminished the ethanol effect. Surprisingly, the ethanol-induced augmentation of IPSPs was completely obliterated by KOR blockade. Our results reveal an important role of the dynorphin/KOR system in the regulation of inhibitory transmission and mediation of ethanol effects in the CeA.
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- 2014
9. Enhanced GABAergic transmission in the central nucleus of the amygdala of genetically selected Marchigian Sardinian rats: Alcohol and CRF effects
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Roberto Ciccocioppo, Markus Heilig, George F. Koob, Christopher S. Oleata, George Luu, Melissa A. Herman, Marisa Roberto, and Marsida Kallupi
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Male ,medicine.medical_specialty ,Corticotropin-Releasing Hormone ,Biology ,Neurotransmission ,Pharmacology ,Inhibitory postsynaptic potential ,Synaptic Transmission ,Article ,Rats, Sprague-Dawley ,Cellular and Molecular Neuroscience ,Corticotropin-releasing hormone ,Species Specificity ,Postsynaptic potential ,Internal medicine ,medicine ,Animals ,GABAergic Neurons ,Rats, Wistar ,Ethanol ,GABAA receptor ,Central nucleus of the amygdala ,Antagonist ,Amygdala ,Receptors, GABA-A ,Rats ,Alcoholism ,Endocrinology ,Inhibitory Postsynaptic Potentials ,GABAergic - Abstract
The GABAergic system in the central amygdala (CeA) plays a major role in ethanol dependence and the anxiogenic-like response to ethanol withdrawal. Alcohol dependence is associated with increased corticotropin releasing factor (CRF) influence on CeA GABA release and CRF type 1 receptor (CRF 1 ) antagonists prevent the excessive alcohol consumption associated with dependence. Genetically selected Marchigian Sardinian (msP) rats have an overactive extrahypothalamic CRF 1 system, are highly sensitive to stress, and display an innate preference for alcohol. The present study examined differences in CeA GABAergic transmission and the effects of ethanol, CRF and a CRF 1 antagonist in msP, Sprague Dawley, and Wistar rats using an electrophysiological approach. We found no significant differences in membrane properties or mean amplitude of evoked GABA A -inhibitory postsynaptic potentials (IPSPs). However, paired-pulse facilitation (PPF) ratios of evoked IPSPs were significantly lower and spontaneous miniature inhibitory postsynaptic current (mIPSC) frequencies were higher in msP rats, suggesting increased CeA GABA release in msP as compared to Sprague Dawley and Wistar rats. The sensitivity of spontaneous GABAergic transmission to ethanol (44 mM), CRF (200 nM) and CRF 1 antagonist (R121919, 1 μM) was comparable in msP, Sprague Dawley, and Wistar rats. However, a history of ethanol drinking significantly increased the baseline mIPSC frequency and decreased the effects of a CRF 1 antagonist in msP rats, suggesting increased GABA release and decreased CRF 1 sensitivity. These results provide electrophysiological evidence that msP rats display distinct CeA GABAergic activity as compared to Sprague Dawley and Wistar rats. The elevated GABAergic transmission observed in naive msP rats is consistent with the neuroadaptations reported in Sprague Dawley rats after the development of ethanol dependence.
- Published
- 2013
10. Sex differences in responses of the basolateral-central amygdala circuit to alcohol, corticosterone and their interaction
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Marisa Roberto, Marian L. Logrip, and Christopher S. Oleata
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0301 basic medicine ,Male ,medicine.medical_specialty ,Inhibitory postsynaptic potential ,Amygdala ,Article ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,Glutamatergic ,chemistry.chemical_compound ,0302 clinical medicine ,Postsynaptic potential ,Corticosterone ,Internal medicine ,medicine ,Animals ,Rats, Wistar ,Pharmacology ,Neurons ,Sex Characteristics ,Ethanol ,business.industry ,Basolateral Nuclear Complex ,Central Amygdaloid Nucleus ,Excitatory Postsynaptic Potentials ,Rats ,Electrophysiology ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,chemistry ,Female ,business ,Nucleus ,030217 neurology & neurosurgery - Abstract
Alcohol use disorders are chronically relapsing conditions that pose significant health challenges for our society. Stress is a prevalent trigger of relapse, particularly for women, yet the mechanisms by which alcohol and stress interact, and how this differs between males and females, remain poorly understood. The glutamatergic circuit connecting the basolateral (BLA) and central (CeA) nuclei of the amygdala is a likely locus for such adaptations, yet the impact of alcohol, corticosterone and their interaction on this circuit has been understudied. In particular, no studies have addressed sex differences in these effects or potential differential responses between the lateral and medial subdivisions of the central nucleus. Thus, we assessed the effects of alcohol and corticosterone treatments on BLA-evoked compound glutamatergic responses in medial and lateral CeA neurons from male and female rats. We observed minimal differences between medial and lateral CeA responses to alcohol and corticosterone in male rats, which were primarily sensitive to alcohol-induced inhibition of glutamatergic postsynaptic potentials. Unlike male neurons, cells from female rats displayed reduced sensitivity to alcohol's inhibitory effects. In addition, female neurons diverged in their sensitivity to corticosterone, with lateral CeA neuronal responses significantly blunted following corticosterone treatment and medial CeA neurons largely unchanged by corticosterone or subsequent co-application of alcohol. Together these data highlight striking differences in how male and female amygdala respond to alcohol and the stress hormone corticosterone, factors which may impact differential susceptibility of the sexes to alcohol- and stress-related disorders.
- Published
- 2016
11. New insights on neurobiological mechanisms underlying alcohol addiction
- Author
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Thomas L. Kash, Veronica A. Alvarez, Changhai Cui, Hitoshi Morikawa, Karen K. Szumlinski, Antonio Noronha, Garret D. Stuber, Mark V. Wilcox, and Marisa Roberto
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Pharmacology ,Neuronal Plasticity ,Alcohol addiction ,Addiction ,media_common.quotation_subject ,Alcohol dependence ,Binge drinking ,Brain ,Article ,Review article ,Binge Drinking ,Behavior, Addictive ,Cellular and Molecular Neuroscience ,Alcoholism ,Metaplasticity ,Neuroplasticity ,Structural plasticity ,Animals ,Humans ,Nerve Net ,Psychology ,Neuroscience ,Stress, Psychological ,media_common - Abstract
Alcohol dependence/addiction is mediated by complex neural mechanisms that involve multiple brain circuits and neuroadaptive changes in a variety of neurotransmitter and neuropeptide systems. Although recent studies have provided substantial information on the neurobiological mechanisms that drive alcohol drinking behavior, significant challenges remain in understanding how alcohol-induced neuroadaptations occur and how different neurocircuits and pathways cross-talk. This review article highlights recent progress in understanding neural mechanisms of alcohol addiction from the perspectives of the development and maintenance of alcohol dependence. It provides insights on cross talks of different mechanisms and reviews the latest studies on metaplasticity, structural plasticity, interface of reward and stress pathways, and cross-talk of different neural signaling systems involved in binge-like drinking and alcohol dependence.
- Published
- 2012
12. Endocannabinoids restrict hippocampal long-term potentiation via CB1
- Author
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Paul Schweitzer, Kristen A. Slanina, and Marisa Roberto
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Male ,Cannabinoid receptor ,Patch-Clamp Techniques ,medicine.medical_treatment ,Long-Term Potentiation ,Thiazines ,Hippocampal formation ,Neurotransmission ,In Vitro Techniques ,Inhibitory postsynaptic potential ,Meloxicam ,Hippocampus ,Membrane Potentials ,Rats, Sprague-Dawley ,Cellular and Molecular Neuroscience ,Receptor, Cannabinoid, CB1 ,Cannabinoid Receptor Modulators ,LTP induction ,medicine ,Animals ,Cyclooxygenase Inhibitors ,Theta Rhythm ,Pharmacology ,Cyclooxygenase 2 Inhibitors ,Chemistry ,musculoskeletal, neural, and ocular physiology ,Excitatory Postsynaptic Potentials ,Long-term potentiation ,Electric Stimulation ,Rats ,Electrophysiology ,Thiazoles ,nervous system ,Cyclooxygenase 2 ,Prostaglandin-Endoperoxide Synthases ,Excitatory postsynaptic potential ,Cannabinoid ,Neuroscience ,Endocannabinoids - Abstract
Cannabinoid ligands alter cognition and prevent long-term potentiation (LTP) of synaptic transmission, but the influence of endogenously formed cannabinoids (eCBs) on hippocampal LTP remains ambiguous. In the accompanying study, we showed that eCB levels regulated by cyclooxygenase-2 (COX-2) tonically decrease basal excitatory transmission. Here, we investigated the influence of eCBs on LTP in CA1 hippocampus. LTP elicited by moderate stimulations (20 or 50 pulses) was facilitated in slices treated with a CB1 antagonist, whereas LTP elicited with robust stimulations (100 or 200 pulses) was unchanged by CB1 blockade. LTP elicited with theta-burst stimulations also was facilitated with CB1 blockade, revealing a tonic inhibitory influence of eCBs on LTP induction. Conversely, inhibition of COX-2 prevented LTP elicited with theta burst stimulations. Inhibition of COX-1 or other routes of eCB degradation did not affect LTP. We conclude that COX-2 regulates the formation of CB1 ligands that negatively regulate LTP.
- Published
- 2005
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