1. Regulated expression of the neuronal calcium sensor-1 gene during long-term potentiation in the dentate gyrus in vivo
- Author
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H Meziane, John C. Roder, Sabrina Davis, Jacques Mallet, Andreas Jeromin, A. Genin, Serge Laroche, Valérie Doyère, CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Neurobiologie de l'apprentissage, de la mémoire et de la communication (NAMC), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Institut de neurosciences cognitives de la méditerranée - UMR 6193 (INCM), Université de la Méditerranée - Aix-Marseille 2-Centre National de la Recherche Scientifique (CNRS), Mount Sinai Hospital [Toronto, Canada] (MSH), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)
- Subjects
Male ,[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology ,Long-Term Potentiation ,MESH: Neurons ,MESH: Rats, Sprague-Dawley ,MESH: Calcium-Binding Proteins ,MESH: Neuropeptides ,Synaptic Transmission ,Rats, Sprague-Dawley ,0302 clinical medicine ,Calcium-binding protein ,Gene expression ,MESH: Up-Regulation ,MESH: Animals ,In Situ Hybridization ,Calcium signaling ,Neurons ,Regulation of gene expression ,0303 health sciences ,MESH: Neuronal Calcium-Sensor Proteins ,[SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior ,General Neuroscience ,MESH: Electric Stimulation ,[SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences ,Long-term potentiation ,MESH: Gene Expression Regulation ,Up-Regulation ,MESH: Calcium ,MESH: Rats ,Neuronal Calcium-Sensor Proteins ,Biology ,MESH: Calcium Signaling ,03 medical and health sciences ,MESH: In Situ Hybridization ,MESH: Long-Term Potentiation ,MESH: Synaptic Transmission ,Animals ,Calcium Signaling ,RNA, Messenger ,MESH: RNA, Messenger ,030304 developmental biology ,Dentate gyrus ,Calcium-Binding Proteins ,Neuropeptides ,Electric Stimulation ,MESH: Male ,Rats ,Gene Expression Regulation ,Neuronal calcium sensor-1 ,Dentate Gyrus ,Synaptic plasticity ,biology.protein ,Calcium ,Neuroscience ,030217 neurology & neurosurgery ,MESH: Dentate Gyrus - Abstract
International audience; Neuronal calcium sensor-1 (NCS-1), the mammalian homologue of frequenin, is a member of a highly conserved family of neuron-specific calcium-binding proteins which has been implicated in exocytosis and in multiple calcium-signalling pathways, suggesting a potential involvement in mechanisms of neuronal plasticity. Here, using in situ hybridization, we report an increased induction of the mRNA encoding NCS-1 in dentate granule cells following the induction of long-term potentiation in the awake rat. We show that NCS-1 mRNA levels are increased 1 and 3 h after long-term potentiation in an N-methyl-D-aspartate receptor-dependent manner, returning to baseline expression levels by 6 h. Electroconvulsive stimulation also induced NCS-1 mRNA transcription in the dentate gyrus, but at the different time of 6 h post-seizure, returning to baseline by 12 h. These results show that regulated expression of the NCS-1 gene is part of the transcriptional response associated with activity-dependent neuronal plasticity in vivo and suggest a molecular mechanism capable of mediating a functional change in synapse sensitivity to calcium and calcium-signalling pathways after long-term potentiation.
- Published
- 2001