1. Trophic activity derived from bone marrow mononuclear cells increases peripheral nerve regeneration by acting on both neuronal and glial cell populations.
- Author
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Ribeiro-Resende VT, Pimentel-Coelho PM, Mesentier-Louro LA, Mendez RM, Mello-Silva JP, Cabral-da-Silva MC, de Mello FG, de Melo Reis RA, and Mendez-Otero R
- Subjects
- Animals, Bone Marrow Cells physiology, Bromodeoxyuridine metabolism, Cell Death, Cell Proliferation, Cells, Cultured, Chick Embryo, Disease Models, Animal, Ganglia, Spinal cytology, Male, Nerve Growth Factor therapeutic use, Nerve Regeneration drug effects, Nerve Tissue Proteins metabolism, Neurons classification, Neurons drug effects, Rats, Sciatic Neuropathy drug therapy, Tissue Culture Techniques, Bone Marrow Transplantation methods, Nerve Regeneration physiology, Neuroglia physiology, Neurons physiology, Sciatic Neuropathy pathology, Sciatic Neuropathy surgery
- Abstract
A rat model of complete sciatic nerve transection was used to evaluate the effect of bone marrow mononuclear cells (BMMC) transplanted to the injury site immediately after lesion. Rats treated with BMMC had both sensory and motor axons reaching the distal stump earlier compared to untreated animals. In addition, BMMC transplantation reduced cell death in dorsal root ganglia (DRG) compared to control animals. Transplanted BMMC remained in the lesion site for several days but there is no evidence of BMMC differentiation into Schwann cells. However, an increase in the number of Schwann cells, satellite cells and astrocytes was observed in the treated group. Moreover, neutralizing antibodies for nerve growth factor (NGF) (but not for brain-derived neurotrophic factor and ciliary-derived neurotrophic factor) added to the BMMC-conditioned medium reduced neurite growth of sensory and sympathetic neurons in vitro, suggesting that BMMC release NGF, improve regeneration of the sciatic nerve in the adult rat and stimulate Schwann and satellite cell proliferation or a combination of both.
- Published
- 2009
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