1. Confocal and multiphoton calcium imaging of the enteric nervous system in anesthetized mice
- Author
-
Keiko Gengyo-Ando, Minoru Suzuki, Yasuyuki Fusamae, Masaaki Sato, Miyako Takaki, Yuki Motegi, Yuji Ikegaya, Junichi Nakai, Masamichi Ohkura, Yoshie Hongo, Koichi Ogawa, and Kazuhide Horiguchi
- Subjects
0301 basic medicine ,Male ,Serotonin ,Intravital Microscopy ,Confocal ,Mice, Transgenic ,Biology ,Enteric Nervous System ,law.invention ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Calcium imaging ,Two-photon excitation microscopy ,In vivo ,Confocal microscopy ,law ,Animals ,Neurons ,General Neuroscience ,General Medicine ,Cell biology ,Molecular Imaging ,Intestines ,030104 developmental biology ,Microscopy, Fluorescence ,Enteric nervous system ,Calcium ,mCherry ,030217 neurology & neurosurgery ,Preclinical imaging - Abstract
Most imaging studies of the enteric nervous system (ENS) that regulates the function of the gastrointestinal tract are so far performed using preparations isolated from animals, thus hindering the understanding of the ENS function in vivo. Here we report a method for imaging the ENS cellular network activity in living mice using a new transgenic mouse line that co-expresses G-CaMP6 and mCherry in the ENS combined with the suction-mediated stabilization of intestinal movements. With confocal or two-photon imaging, our method can visualize spontaneous and pharmacologically-evoked ENS network activity in living animals at cellular and subcellular resolutions, demonstrating the potential usefulness for studies of the ENS function in health and disease.
- Published
- 2018