1. Protective effect of planarian DJ-1 against 6-hydroxydopamine-induced neurotoxicity
- Author
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Yoshihisa Kitamura, Natsuka Tashiro, Kaneyasu Nishimura, Jun Tsushima, Hiroyoshi Ariga, Kiyokazu Agata, Kazuyuki Takata, Kanji Yoshimoto, and Eishi Ashihara
- Subjects
Blotting, Western ,Molecular Sequence Data ,Protein Deglycase DJ-1 ,Fluorescent Antibody Technique ,In situ hybridization ,Neuroblastoma ,Adrenergic Agents ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Neurotoxin ,Amino Acid Sequence ,Oxidopamine ,In Situ Hybridization ,Oncogene Proteins ,Hydroxydopamine ,Sequence Homology, Amino Acid ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,Dopaminergic Neurons ,General Neuroscience ,Intracellular Signaling Peptides and Proteins ,PARK7 ,Neurotoxicity ,Planarians ,General Medicine ,medicine.disease ,biology.organism_classification ,Molecular biology ,Planarian ,Dugesia japonica ,Stem cell - Abstract
DJ-1/PARK7 has multiple functions as an antioxidant, an oncogene, and a molecular chaperone in vertebrates, and loss-of-function mutations in DJ-1 cause early onset of Parkinson's disease. However, the function of invertebrate DJ-1 remains unknown. In order to investigate the function of planarian DJ-1, we isolated the planarian DJ-1 gene Dugesia japonica DJ-1 (DjDJ-1) and analyzed its expression and function. In situ hybridization analysis revealed that DjDJ-1 mRNA was expressed throughout the body, including the central nervous system, cells surrounding the pharynx, and stem cells. Planarian DjDJ-1 protein exhibited antioxidant function, similar to human DJ-1, as evidenced by the fact that recombinant DjDJ-1 protein reduced reactive oxygen species and protected human neuroblastoma SH-SY5Y cells from cell death. In addition, dopaminergic neurons in DjDJ-1(RNAi) planarians became susceptible to 6-hydroxydopamine, a dopaminergic neurotoxin. These results suggest that planarians have a DJ-1 ortholog, which has conserved antioxidant and neuroprotective functions.
- Published
- 2012