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Combining several cultivars within species in seed mixtures is often advocated as a means for improving crop or pasture performance. Herbage accumulation (HA) of perennial ryegrass-white clover pastures sown with a single cultivar representing different functional types of perennial ryegrass was compared with the HA of cultivar mixtures over three years in two contrasting environments (Waikato, dryland; Canterbury, irrigated) under dairy cattle grazing. Functional types were: mid-season flowering diploid, late-season diploid, and late-season tetraploid. Mixture treatments (n = 4 at each site) included all binary combinations plus the three-way combination. Mixture HA and the mean HA of the constituent cultivars did not differ for any of the mixture combinations in either environment, apart from winter in Waikato where the mid-diploid plus tetraploid mix out-yielded the mean of the individual cultivars and tended to increase total annual HA. The total annual HA of the mixtures was between 1.0 and 2.3 t DM/ha/year less than the HA of the highest-yielding individual cultivar included in the mixture in six of the eight mixture-environment combinations. While some benefits of mixtures for controlling grazing outcomes were observed, sowing individual high-performing cultivars is a better overall strategy than mixing cultivars to cover a spectrum of traits. [ABSTRACT FROM AUTHOR]

The objective of this study was to investigate the relationship between plasma amino acid (AA) concentration and urinary nitrogen (N) concentration of dairy heifers offered cut plantain or perennial ryegrass-white clover pasture indoors. Twelve Jersey-Friesian heifers aged 9–10 months were equally assigned to two dietary treatments (plantain vs. perennial ryegrass-white clover pasture) in a completely randomised design. Urinary N concentration was lower in plantain fed heifers (1.4 g/kg) than in perennial ryegrass-white clover pasture fed heifers (3.4 g/kg). Feeding cut plantain or perennial ryegrass-white clover pasture did not have an effect on either plasma essential AA, non-essential AA or total AA. There was no association found between plasma AA concentration and urinary N concentration in the present study. Therefore, the urinary N concentration reduction in plantain fed heifers compared with perennial ryegrass-white clover pasture cannot be attributed by the differences in plasma AA. [ABSTRACT FROM AUTHOR]

In New Zealand, a commonly sown pasture combination is perennial ryegrass with white clover. The hydrothermal time model (HTT) was used to quantify germination under different temperature (T) and water potential (WP) conditions for white clover 'Nomad' and perennial ryegrass 'Stellar AR1'. Seeds were germinated in vitro under a range of T × WP combinations, and HTT models were fitted to germination time course data. Rapid germination of white clover under optimum T and WP was reflected by a much lower value for its HTT constant compared with perennial ryegrass (10.8 vs 80.7 MPa °C d). However, the lower base water potential for perennial ryegrass (−1.29 vs −0.495 MPa) and a higher threshold temperature for thermoinhibition (21.8 vs 18.3°C), meant that predicted perennial ryegrass germination was more resilient in warm dry conditions. HTT models can be used to assist decisions about the timing of perennial ryegrass and white clover sowing. [ABSTRACT FROM AUTHOR]

This experiment investigated the feasibility of using herb-clover mixes to enable early weaning of lambs at ∼60 days of age. Over two years, twin lambs weighing more than 16 kg were allocated to three treatments: ryegrass pastures until 90 days-of-age, herb-clover mix until 90 days-of-age or weaned at ∼60 days of age onto herb-clover mix. In 2014, lambs offered the herb-clover mix, weaned at 60 or 90 days, were heavier at 90 days than lambs grazing on pasture with their dams. In 2015, lambs that weaned early onto the herb-clover mix were lighter at the same age as lambs that remained with their dam, but they still achieved a commercial weight. Ewes weaned early were heavier at weaning than ewes suckling lambs on ryegrass pasture in both years. Under some conditions, early weaning of lambs onto a herb-clover mix can be an effective alternative to conventional weaning on ryegrass pasture. [ABSTRACT FROM AUTHOR]

The objective of this study was to quantify amino acid (AA) profiles in plasma collected from dairy heifers grazing swards containing ryegrass/clover, chicory and plantain in autumn and spring. The study comprised a 28-day autumn trial and a 21-day spring trial using 56 Friesian x Jersey heifers. Total AA concentrations ranged from 2849 to 3165 μM in autumn and from 3195 to 3454 μM in spring, with no treatment effect. Season affected essential AA (EAA) and total AA concentrations. Total plasma AA concentrations were lower in autumn (2965 μM) than in spring (3303 μM). Based on these results, feeding dairy heifer's either pure chicory, binary mixture or pure plantain does not affect EAA and total AA of Friesian x Jersey heifers. Further, no relationship was established between AA and weight gain of heifers in this study. [ABSTRACT FROM AUTHOR]

Pasture mitigation strategies are needed to reduce the negative effects of nitrate (NO3−) leaching from intensive pastoral livestock production systems. We explored the capability of pasture grasses with different cool season (winter-early spring) growth rates to reduce NO3− leaching and increase N uptake. Following a single urine application of 700 kg N ha−1, NO3− leaching loss and N uptake by Italian ryegrass and four perennial ryegrass cultivars of varying seasonal growth (heading dates from -7 to +20 days), either undersown with/without Italian ryegrass, were quantified in a 12 month lysimeter study. Italian ryegrass cv. Tabu had lower NO3−-N leaching loss and drainage, and greater N uptake and DM yield than perennial ryegrass varieties. Italian ryegrass total N leaching loss (143 kg N ha−1 yr−1) was 46-33% less (P < .05) than early and mid-season maturing Tyson, Arrow and AberDart perennial ryegrass. Italian ryegrass total N uptake (463 kg N ha−1) was 1.2-1.4 times greater than all other grass sward types. Overall, perennial ryegrass pure swards had similar N uptake and N leaching. Late-season maturing One 50 perennial ryegrass showed significantly reduced (P < .05) total N leaching loss. Undersowing Italian ryegrass into established perennial ryegrass swards resulted in lower NO3− leaching and greater N uptake than non-undersown perennial ryegrass swards, though not significant. Results confirm Italian ryegrass is a useful pasture grass option for reducing annual N leaching loss. Cool season perennial ryegrass varieties appear not to influence soil N uptake and leaching loss. [ABSTRACT FROM AUTHOR]

Herbage accumulation (HA) data from four sites were combined to (1) address the hypothesis that adjustments to the New Zealand Forage Value Index to account for grass-clover interactions are not required and (2) explore the main effects of clover and nitrogen (N) fertiliser treatments on pasture productivity. In summer and autumn, pastures based on mid-season heading ryegrass cultivars consistently supported a greater clover content than pastures based on late heading cultivars. However, the clover ×  cultivar interaction was significant in only 2 of 17 HA data sets available for analysis. Thus, the hypothesis is supported. Compared with ryegrass monocultures, mixtures yielded an additional 2.4 (in the Low-N treatment) and 1.2 (High-N treatment) t DM/ha/year. Apparent N transfer from clover to grass was 79 (Low N) and 27 (High N) kg N/ha/year, derived from estimated N fixation efficiencies of 66 (Low N) and 50 (High N) kg N/t clover DM grown. [ABSTRACT FROM AUTHOR]

The implementation and monitoring of the treatments in the core experiment (eight perennial ryegrass cultivars grown under four combinations of plus/minus clover and high/low nitrogen (N)) at a dryland Waikato site is described. The N x clover interaction was significant in 10 of the 17 seasonal or total annual herbage accumulation (HA) data sets available, caused by substantially lower annual HA in the low N minus clover treatment compared with all other treatments. Five significant scaling-type clover × cultivar interactions in HA were evident. In all cases, HA was greater in plus clover than minus clover treatments, but more so for some cultivars than others. However, the interactions were not consistent across seasons, and different cultivars or phenotypic contrast groups were involved in many cases. The hypothesis that relative HA rankings of ryegrass cultivars do not differ when ryegrass is grown in monoculture versus mixtures with white clover is supported. [ABSTRACT FROM AUTHOR]

This experiment, implemented at a Southland site representative of the lower South Island, New Zealand, and monitored for three full years from 2012/2013 to 2015/2016, was one of four throughout New Zealand that investigated the yield and nutritive value rankings of perennial ryegrass cultivars sown with or without white clover and with high (225 kg N/ha/annum) or low (50 kg N/ha/annum) application rates of nitrogen fertiliser. High inputs of nitrogen (N) fertiliser increased annual total dry matter (DM) yields and seasonal yield in 12 out of 18 measurement occasions. Adding white clover increased both annual dry matter yield and seasonal yield in 7 out of 18 measurement occasions. An interaction between clover and N input occurred on 10 occasions. Over time, the low N minus clover treatment became progressively lower yielding compared with the other three treatments. Ryegrass cultivar by clover interactions occurred in the latter half of the experiment. When analysed using ryegrass characteristics, early- and late-heading ryegrass cultivars produced similar yields when sown without clover, but late-heading ryegrass cultivars had a higher yield than early-heading pastures when sown with white clover (P < .001). Tetraploid ryegrasses had lower yields than diploid ryegrasses when sown without clover (P = .001). This difference was reduced when white clover was included. There was no significant interaction with ryegrass morphology when sown with white clover, as dense ryegrass cultivars always had a higher yield than open ryegrass cultivars (P = .005). Interactions occurred between N input levels and ryegrass cultivar. These occurred in early spring or late spring of each year. Analysis by ryegrass characteristics identified consistent interactions. Ryegrass cultivars with a late-heading date were higher yielding than early-heading cultivars at low N inputs (P = .029), but they were not different at high N inputs. Diploid and tetraploid ryegrass cultivars were similar with low N inputs, but diploid cultivars out-yielded tetraploid cultivars at high N inputs (P = .005). Significant interactions between N inputs and white clover addition affected herbage nutritive value but this did not extend to consistent interactions with ryegrass cultivar. Invertebrate pest numbers were generally low and unlikely to have influenced clover/ryegrass interactions or cultivar ranking. Results from this site suggest that ranking of ryegrass cultivars needs to account for both the inclusion of clover and variation in N fertiliser input. [ABSTRACT FROM AUTHOR]

The development of economic indexing systems for ranking cultivars of forage species requires new knowledge of factors that may influence the scaling of agronomic data collected in controlled, small-plot evaluation trials to whole farm systems. In the case of perennial ryegrass indexing, one such knowledge gap is the effect of growing clover with ryegrass on the relative rankings of different ryegrass cultivars. The objective of the investigation reported in this series was to test whether the relative yield rankings of perennial ryegrass (Lolium perenne L.) cultivars representing the range in phenotypes now available to New Zealand farmers as a result of recent breeding differed when those cultivars were grown in monocultures (as per standard protocols for cultivar yield trialling systems such as the National Forage Variety Trials) versus mixtures of grass and clover. This paper presents the rationale for the investigation by reviewing developments in perennial ryegrass breeding and evaluation in the context of grass-clover relationships in grazed pasture systems. The papers that follow report results from a multi-year, multi-site investigation comparing the yield, nutritive value and other productivity-related variables of eight perennial ryegrass cultivars grown with or without white clover (monoculture versus mixture respectively) at two levels of nitrogen fertiliser. Key considerations in the design of the experiment on which the investigation was based are discussed. [ABSTRACT FROM AUTHOR]

Growth rates of weaned bull calves in late summer/early autumn can be low in a ryegrass/white clover-based system. This experiment compared the liveweight gain of weaned bull calves (n = 18 per treatment per year, in 3 replicates of 6 bulls) grazed on a herb sward comprised of chicory, plantain, red clover and white clover with bulls grazed on either pasture supplemented with 1.8 kg DM/day of concentrate, or pasture without supplementation. The experiment was repeated over three years. Bulls grazed on the herb sward grew faster (P < .05) than bulls grazed on pasture with or without concentrate supplementation. Liveweight gain of bulls grazed on pasture was less than for bulls supplemented with concentrate in two of the three years (P < .05). A herb sward is a better quality alternative to late summer/early autumn pasture, and could be used to increase liveweight gain of bulls during this period. [ABSTRACT FROM AUTHOR]

Liveweight production was quantified from six dryland pastures at Lincoln University, Canterbury, New Zealand, over an 8 year period. The experiment was a randomised complete block with six replicates. The pastures were established in autumn 2002 with cocksfoot (CF) and subterranean (CF/Sub), balansa (CF/Bal), white (CF/Wc) or Caucasian (CF/Cc) clovers, a perennial ryegrass/white clover (RG/Wc) control and a lucerne (Luc) monoculture. Liveweight (LWt) production from Years 2 (2003–04) to 9 (2010–11) was 7940 kg LWt/ha from the lucerne monoculture, 7270 kg LWt/ha from the CF/Sub pasture and 5680 ± 171 kg LWt/ha from CF/Bal, CF/Wc, CF/Cc and RG/Wc pastures. Annually, LWt production ranged from 443 kg/ha (CF/Wc pastures with hoggets grazing in spring in 2006–07) to 1285 kg/ha from CF/Sub pastures in Year 9 when ewes with twin lambs at foot grazed in spring. On average, grass-based pastures produced 64%–70% of their total annual LWt production in spring compared with 57% from lucerne monocultures. In spring, relationships between LWt and total spring DM yield showed that 7.3 ± 0.68 kg LWt/ha was produced per 100 kg total DM (R2= 0.87) grown in 5 of the 6 years under the hogget/weaned lamb grazing system. Relationships between LWt and metabolisable energy or N yield failed to improve this relationship, which suggests that DM yield from persistent improved pasture components was the major factor responsible for differences in LWt production from these optimally grazed pastures. [ABSTRACT FROM PUBLISHER]

A botanical survey of 24 commercial dairy pastures ranging in post-sowing age and perennial ryegrass type (mid-season diploid, late-season diploid, tetraploid) was undertaken in each of Waikato-Bay of Plenty (BoP), Taranaki and Canterbury during autumn/winter. The mean perennial ryegrass proportion (85% of total DM) and tiller density (3252 m−2) was greatest in Canterbury and the broadleaved weed proportion lowest (2%). Conversely, Waikato–BoP had the lowest ryegrass proportion (59%), tiller density (1817 m−2) and greatest proportion of broadleaved weeds (15%), with Taranaki intermediate. Tiller density was greater in pastures sown with diploid rather than tetraploids (averaging 3033 vs. 1784 m−2). Grass grub (Costelytra zealandica, 11 m−2) populations were lowest in the youngest age category pastures where nematode populations were highest (80 g−1dry soil). The soil seedbank was dominated by unsown species (with total species emergence averaging 4594 seeds m−2), with negligible ryegrass emergence. [ABSTRACT FROM PUBLISHER]

Soil phosphorus (P) concentrations in excess of those required to maximise pasture production impose unnecessary costs on farmers and potentially increase the risk of P runoff to waterways. The relationship between soil available P concentration and pasture production was examined on an Oxyaquic Hydrosol. Mean annual pasture production ranged from 18,415 to 21,834 kg DM/ha over the 3-year sampling period. There was no significant difference in the annual DM production at Olsen P concentrations of 23-81 mg/kg measured from the 0-75 mm sampling depth. Results show that there was no increase in pasture production when soil Olsen P concentrations exceeded 23 mg/kg (0-75 mm). The optimum soil Olsen P ranges determined in the current study are well below the mean paddock concentrations previously measured in northwest Tasmania (54 mg/kg) and suggest that such high concentrations are not justified in terms of optimising pasture growth. [ABSTRACT FROM AUTHOR]

Perennial ryegrass (Lolium perenne L.) can be sown as a mixture of cultivars, but different eco-physiological mechanisms among cultivars can cause the cultivar proportions to change over time. The objectives of this work were to differentiate the cultivars 'Barnhem', 'Barmaco', 'Barlet' and 'Mara', and to identify their proportions in mixture. Two seed-lots of each cultivar were characterised using one simple sequence repeat (SSR) and two inter-SSR (ISSR) markers. Simulated mixtures based on random sub-samples from monocultures of each cultivar showed that individuals could be correctly allocated to their respective seed-lot and cultivar with 78-87% accuracy using discriminant analysis. 100 BG34 plants were collected from three dairy pastures in Ohio, USA and their SSR/ISSR profiles were compared with the reference populations. One pasture had a similar cultivar proportion as the original sown mixture. Two pastures had increased proportions of 'Mara' and 'Barmaco' and decreases of 'Barlet' or 'Barnhem', depending on the pasture. [ABSTRACT FROM AUTHOR]

A lysimeter experiment evaluated the use of sawdust or sucrose to decrease nitrogen (N) leaching from newly deposited urine patches to a perennial ryegrass/white clover pasture on sandy loam soil. Cow urine (equivalent to 500 kg N ha-1), labelled with 15N-urea, was applied to the surface of lysimeters and sawdust (9 t ha-1) or sucrose (3, 12 or 24 t ha-1) was applied immediately afterwards. Sawdust did not decrease mineral N leaching compared with urine-only (82 kg N ha-1). Sucrose applied at 12 or 24 t ha-1 decreased mineral N leaching by 27 or 66%, compared to urine-only. Pasture dry matter yields were decreased in all sucrose treatments (range 16-29% over 247 days, but 15-73% over the first 70 days) compared with urine-only. Total recovery of 15N in pasture and soil was similar across treatments (average 66%) but the 15N partitioning between soil and pasture differed; soil recovery was 19% in the urine-only and urine + sawdust treatments, and 27-51% in the sucrose treatments, increasing with increasing rate of sucrose. In the farm situation, the risk of yield loss and the large carbon applications required may limit the practical application of this N mitigation method. [ABSTRACT FROM AUTHOR]

The objective of this study was to improve the McCall herbage growth model. A more mechanistic senescence function was incorporated, replacing an empirical senescence function. The new function is based on leaf lifespan (LLS), measured in thermal time, which is characteristic of a given grass species, and can be measured independently or sourced from the literature. Other complementary changes were also incorporated, the major ones being to incorporate soil water infiltration constraints and to improve seasonal predictions in winter based on greater photosynthetic efficiency of leaves at low light intensity. The existing and new versions of the model were each calibrated against five time series of herbage growth data using the first half of each time series. The second half of each time series was used for validation, along with an independent dataset. A suite of goodness-of-fit indicators was used to evaluate and compare the existing and new versions of the model. The new version more accurately predicted herbage growth following calibration, showing higher concordance correlation coefficients (ρ c = 0.59, 0.62, 0.81, 0.81 and 0.53 versus 0.85, 0.88, 0.81, 0.88 and 0.80, for existing and new versions respectively). Most goodness-of-fit indicators also improved for the independent dataset (e.g. ρ c = 0.708 versus 0.787, for existing and new versions, respectively). It is concluded that the new improved version of the model can be confidently used as a replacement for the existing version [ABSTRACT FROM AUTHOR]

Gibberellins are plant hormones that activate dormant enzyme systems. Applied to pasture, they can stimulate out-of-season growth or accelerate growth through reserve mobilisation, leaf and stem elongation, and promotion of flowering. When evaluating gibberellin as a potential pasture production stimulant, historical investigations frequently used rates ranging from 25-700 g/ha of active ingredient. Responses were found to be uneconomic, and there were often side effects including a decrease in tillering, decreased root mass, and a yield depression subsequent to the initial response. With gibberellic acid now available from China at comparatively cheaper prices than applied historically, and emerging understanding that application rates as low as 5-10 g/ha active ingredient can produce more cost-effective responses, possibly with reduced side effects, there is renewed interest in gibberellin use to increase winter and spring pasture production or manipulate seasonality of production. a rationale for delivery of extension advice is presented. [ABSTRACT FROM AUTHOR]

Rhizobium populations were counted in random soil samples from a ryegrass/clover pasture, in soil around white clover plants, and also in the rhizoplane of the white clover roots. The random soil samples contained 3 x 105 cells per g dry soil of Rhizobium leguminosarum bv. trifolii effective on its host, and the same number of Mesorhizobium loti. Soil around white clover plants had 106 cells per g dry soil of each Rhizobium. In the rhizoplane of white clover there were 106 effective R. leguminosarum bv. trifolii per mg root dry weight, and only 10³ M. loti per mg root dry weight. The increase in the ratio of effective white clover rhizobia to M. loti in the rhizoplane demonstrates species-specific stimulation of effective rhizobia on the root surface. This would be important for the ongoing cycles of effective nodulation required by the clonal growth habit of white clover in pasture. [ABSTRACT FROM AUTHOR]