Acute and chronic experiments were conducted to evaluate the effect of salinity on survival and growth of the juvenile Dark Sleeper Odontobutis potamophila. For the acute experiment, half-lethal concentrations (LC50) of salinity for the juvenile Dark Sleeper after exposures for 12, 24, 48, 72 and 96 h were determined. Mean survival time (MST) and half-survival time (ST50) at salinity of 32‰ were also recorded. For the chronic experiment, a 22-day trial focused on comparing the effects of different salinity levels (0, 2, 4, 6, 8, and 10‰) on survival rate and growth of the juvenile Dark Sleeper. Results revealed that the 96-h LC50 was 13.79‰ salinity. MST and ST50 at 32‰ were 35.4 min and 37.0 min, respectively. In the 22-d trial, survival rate and specific growth rate (SGR) decreased with salinity. Survival rate of groups in 8‰ and 10‰ salinity were significantly lower than that of other groups. The SGR for body length and body weight of the 10‰ group was significantly lower than that of the othe...