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To inquire about the possibility of durable valorization of saffron, this review highlights the different techniques of in vitro culture and varietal creation of this specie. Then, a reveal main component of saffron with some pharmacological activities that make this product a serious therapeutic hope. Saffron (Crocus sativus L.) is a sterile triploid geophyte that propagated by corms. To respond to the increasing global demand for saffron, it is necessary to expand the area under cultivation. Thus, in vitro techniques can produce a large quantity of propagating material in reduced time. It is well-known that saffron is traditionally used as a coloring or flavoring agent, but recent research showed its potential for health promotion. The interest components include crocin, crocetin, picrocrocin, and safranal have all demonstrated a wide range of use in medical field. Previous studies have reported that biological activities of saffron alleviates or prevents health problems such as stomach upset, cardiovascular disease, and depression. In addition, saffron is also promising in cancer prevention due to its antioxidant properties.