1. Multi-focal sequencing of a diffuse intrinsic pontine glioma establishes PTEN loss as an early event
- Author
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Marcia Leonard, Stefanie Stallard, Pedro R. Lowenstein, Carl Koschmann, Sriram Venneti, Xuhong Cao, Valerie P. Opipari, Katayoon Kasaian, Daniel Zamler, Hugh J. L. Garton, Rajen Mody, Karin M. Muraszko, Luigi Franchi, Arul M. Chinnaiyan, Shawn L. Hervey-Jumper, Patricia L. Robertson, Maria G. Castro, and Zishaan Farooqui
- Subjects
0301 basic medicine ,Cancer Research ,Pathology ,medicine.medical_specialty ,Case Report ,Somatic evolution in cancer ,lcsh:RC254-282 ,Germline ,03 medical and health sciences ,0302 clinical medicine ,Glioma ,medicine ,PTEN ,Tensin ,Exome ,PI3K/AKT/mTOR pathway ,biology ,medicine.disease ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,3. Good health ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,biology.protein ,Cancer research ,Brainstem - Abstract
Improved molecular understanding is needed for rational treatment of diffuse intrinsic pontine gliomas (DIPG). Here, using multi-focal paired tumor and germline exome DNA and RNA sequencing, we uncovered phosphatase and tensin homolog (PTEN) loss as a clonal mutation in the case of a 6-year-old boy with a diffuse intrinsic pontine glioma, and incorporated copy number alteration analyses to provide a more detailed understanding of clonal evolution in diffuse intrinsic pontine gliomas. As well, using the PedcBioPortal, we found alterations in PTEN in 16 of 326 (4.9%) cases of pediatric high-grade glioma (3 of 154 (1.9%) brainstem) for which full sequencing data was available. Our data strengthens the association with PTEN loss in diffuse intrinsic pontine gliomas and provides further argument for the inclusion of PTEN in future targeted sequencing panels for pediatric diffuse intrinsic pontine gliomas and for the development and optimization of mTOR/PI3K inhibitors with optimal central nervous system penetration.
- Published
- 2017
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