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Start Over Author "Dang Van Phu" Journal nuclear science and technology
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2. Radiation-crosslinked scaffolds from gelatin/CM-chitin and gelatin/CM-chitosan hydrogels for adipose-derived stem cell culture

Author

Nguyen Thi Ly, Tran Le Bao Ha, Dang Van Phu, Nguyen Ngoc Duy, Nguyen Thi Kim Lan, Nguyen Quoc Hien, Le Anh Quoc, and Phan Phuoc Thang

Subjects
food.ingredient, technology, industry, and agriculture, Adipose tissue, CM-chitosan, macromolecular substances, Gelatin, carbohydrates (lipids), chemistry.chemical_compound, food, Chitin, chemistry, Cell culture, Self-healing hydrogels, Biophysics
Abstract

Radiation technology has been providing a useful tool to modify polymers involving the radiation crosslinking of hydrogels. In the present work, two kinds of hydrogels composed gelatin and carboxymethyl chitosan (gelatin/CM-chitosan), and carboxymethyl chitin (gelatin/CM-chitin) were prepared by the radiation crosslinking. The resulting hydrogels were freeze-dried, sterilized under gamma-ray at a sterilization dose of 25 kGy, and characterized to be utilized as the scaffolds for culturing adipose-derived stem cells (hADSCs). The physicochemical properties, biodegradability, and cytotoxicity of these scaffolds were also investigated. The results indicated that both CM-chitosan and CM-chitin much improved the swelling capacity, porosity, and pore size of the gelatin-derived scaffolds. The swelling degree of gelatin/CM-chitosan and gelatin/CM-chitin scaffolds was about 7-9 g/g after 6 hrs immerging in PBS, and their porosity was about 70-73% with pore size ranging from 100 to 300 mm. The results also revealed that the compressive modulus of gelatin/CM-chitosan and gelatin/CM-chitin was 45.6 and 66.4 kPa, respectively, which were suitable for soft tissue engineering. Both scaffolds were enzymatically biodegradable by collagenase and non-cytotoxic for hADSCs with an RGR of ~97%. Thus, the resultant scaffolds can be suitably utilized for culturing hADSC in practice to regenerate soft tissues.

Published
2020
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