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Start Over Author "Beckmann, Benedikt" Journal nucleic acids research
4 results on '"Beckmann, Benedikt"'

4. Northern blot detection of endogenous small RNAs (approximately14 nt) in bacterial total RNA extracts.

Author

Beckmann BM, Grünweller A, Weber MH, and Hartmann RK

Subjects
Bacillus subtilis genetics, Base Sequence, Buffers, Cross-Linking Reagents, Escherichia coli genetics, Ethyldimethylaminopropyl Carbodiimide chemistry, Molecular Sequence Data, Nucleic Acid Conformation, Nucleic Acid Denaturation, Oligoribonucleotides chemistry, RNA, Bacterial chemistry, RNA, Untranslated chemistry, Blotting, Northern methods, RNA, Bacterial analysis, RNA, Untranslated analysis
Abstract

Here we describe a northern blot procedure that allows the detection of endogenous RNAs as small as approximately 14 nt in total RNA extracts from bacteria. RNAs that small and as part of total bacterial RNA extracts usually escape detection by northern blotting. The approach combines LNA probes 5'-digoxigenin-endlabeled for non-radioactive probe detection with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide-mediated chemical crosslinking of RNAs to nylon membranes, and necessitates the use of native PAGE either with the TBE or MOPS buffer system.

Published
2010
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