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Your search keyword '"Fondation pour la Recherche Medicale (FRM)"' showing total 2 results
Start Over Author "Fondation pour la Recherche Medicale (FRM)" Journal nucleic acids research
2 results on '"Fondation pour la Recherche Medicale (FRM)"'

1. The somatic piRNA pathway controls germline transposition over generations

Author

Barckmann, Bridlin, El-Barouk, Marianne, Pélisson, Alain, Mugat, Bruno, Li, Blaise, Franckhauser, Céline, Fiston Lavier, Anna-Sophie, Mirouze, Marie, Fablet, Marie, Chambeyron, Séverine, Institut de génétique humaine (IGH), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre de Bioinformatique, Biostatistique et Biologie Intégrative (C3BI), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), LGPD, CNRS, Univ Perpignan Via Domitia, Perpignan, France, Eléments transposables, évolution, populations, Département génétique, interactions et évolution des génomes [LBBE] (GINSENG), Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Fondation pour la Recherche medicale (FRM) [DEP20131128518], Centre National de la RechercheScientifique. Funding for open access charge: FRM., Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), École pratique des hautes études (EPHE), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UR226

Subjects
[SDV.GEN]Life Sciences [q-bio]/Genetics, endocrine system, urogenital system, [SDV]Life Sciences [q-bio], Genome, Insect, Ovary, Genome Integrity, Repair and Replication, Aneuploidy, Drosophila melanogaster, Germ Cells, DNA Transposable Elements, Animals, Female, Gene Silencing, RNA, Small Interfering, Signal Transduction
Abstract

International audience; Transposable elements (TEs) are parasitic DNA sequences that threaten genome integrity by replicative transposition in host gonads. The Piwi-interacting RNAs (piRNAs) pathway is assumed to maintain Drosophila genome homeostasis by downregulating transcriptional and post-transcriptional TE expression in the ovary. However, the bursts of transposition that are expected to follow transposome derepression after piRNA pathway impairment have not yet been reported. Here, we show, at a genome-wide level, that piRNA loss in the ovarian somatic cells boosts several families of the endogenous retroviral subclass of TEs, at various steps of their replication cycle, from somatic transcription to germinal genome invasion. For some of these TEs, the derepression caused by the loss of piRNAs is backed up by another small RNA pathway (siRNAs) operating in somatic tissues at the post transcriptional level. Derepressed transposition during 70 successive generations of piRNA loss exponentially increases the genomic copy number by up to 10-fold.

Published
2018
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2. RbpA relaxes promoter selectivity of M. tuberculosis RNA polymerase

Author

Ayyappasamy Sudalaiyadum Perumal, Yangbo Hu, Rishi K. Vishwakarma, Konstantin Brodolin, Zakia Morichaud, Institut de Recherche en Infectiologie de Montpellier (IRIM), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Wuhan Institute of Virology [Wuhan, China], Chinese Academy of Sciences [Wuhan Branch], French National Research Agency [MycoMaster ANR16-CE11-0025-01], CNRS [PRC Russie CNRS/RFBR to K.B.], Fondation pour la recherche medicale (FRM) (to ´ Y.H.) during his stay in the laboratory of K.B., ERASMUS MUNDUS Svaagata fellowship (to A.S.P.), Infectiopole Sud (to R.K.V.). Funding for open access charge: MycoMaster ANR-16-CE11-0025-01., and Konstantin, Brodolin

Subjects
0301 basic medicine, DNA, Bacterial, Models, Molecular, Transcriptional Activation, Protein subunit, Sigma Factor, Protein Structure, Secondary, Substrate Specificity, 03 medical and health sciences, chemistry.chemical_compound, Bacterial Proteins, Transcription (biology), RNA polymerase, Genetics, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Protein Interaction Domains and Motifs, Nucleotide Motifs, Promoter Regions, Genetic, Binding Sites, biology, Lysine, Gene regulation, Chromatin and Epigenetics, Temperature, RNA, RNA-Binding Proteins, Promoter, DNA-Directed RNA Polymerases, Gene Expression Regulation, Bacterial, Mycobacterium tuberculosis, biology.organism_classification, 3. Good health, Cell biology, Kinetics, RNA, Bacterial, 030104 developmental biology, chemistry, Amino Acid Substitution, Mutation, Linker, DNA, Mycobacterium, Protein Binding
Abstract

International audience; The transcriptional activator RbpA associates with Mycobacterium tuberculosis RNA polymerase (MtbRNAP) during transcription initiation, and stimulates formation of the MtbRNAP-promoter open complex (RPo). Here, we explored the influence of promoter motifs on RbpA-mediated activation of MtbRNAP containing the stress-response σB subunit. We show that both the 'extended -10' promoter motif (T-17G-16T-15G-14) and RbpA stabilized RPo and allowed promoter opening at suboptimal temperatures. Furthermore, in the presence of the T-17G-16T-15G-14 motif, RbpA was dispensable for RNA synthesis initiation, while exerting a stabilization effect on RPo. On the other hand, RbpA compensated for the lack of sequence-specific interactions of domains 3 and 4 of σB with the extended -10 and the -35 motifs, respectively. Mutations of the positively charged residues K73, K74 and R79 in RbpA basic linker (BL) had little effect on RPo formation, but affected MtbRNAP capacity for de novo transcription initiation. We propose that RbpA stimulates transcription by strengthening the non-specific interaction of the σ subunit with promoter DNA upstream of the -10 element, and by indirectly optimizing MtbRNAP interaction with initiation substrates. Consequently, RbpA renders MtbRNAP promiscuous in promoter selection, thus compensating for the weak conservation of the -35 motif in mycobacteria.

Published
2018
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