1. Large stacking stability of a base pair-mimic nucleotide on the DNA duplex.
- Author
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Uotani Y, Nakano S, Nakashima S, Anno Y, Fujii M, and Sugimoto N
- Subjects
- Base Sequence, Nucleic Acid Conformation, Thermodynamics, Base Pairing, DNA chemistry, Molecular Mimicry
- Abstract
We synthesized novel adenosine derivatives tethering an aromatic hydrocarbon group by an amido linker. The single adenosine derivatives at 5' dangling end stabilized the DNA duplex of 5'-ATGCGCAT-3' more or equally than Watson-Crick base pair. When the number of the dangling residues increased from one to three, the duplex stability became larger by 1.8-3.6 kcal/mol. When the adenosine derivative was opposite to an abasic site in a DNA duplex, the destabilization by the abasic site was significantly reduced. These observations suggest that the adenosine derivatives developed in this study can stack with a DNA base pair by forming a base pair-mimic geometry.
- Published
- 2003
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