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Start Over Journal nucleic acids symposium series
5 results

1. Random amplified polymorphic DNA observed in Eucalyptus by PCR study with random primers.

Author

Izumi M, Kojima E, Matsuda M, Shimizu T, Murakami A, Tanabe T, and Murakami K

Subjects
Base Sequence, DNA Primers, DNA, Plant genetics, Operon, Seeds, Species Specificity, Trees classification, DNA, Plant chemistry, Polymorphism, Genetic, Random Amplified Polymorphic DNA Technique, Trees genetics
Abstract

A total DNA extracted from Eucalyptus globlus, E. citriodora, or a subspecies of E. gunnii (Maruha Yu-kari) being used as an template, Polymerase Chain Reaction (PCR) was performed with 40 kinds of commercially available random primers (Operon, OPA-01-20 and OPB-01-20). After electrophoresis of PCR-products on agarose gel, we analyzed the band pattern formed by DNA-fragments with 0.5-3.0 kbp as molecular weight. As a remuneration for the examination in which forty 10-mer arbitrary oligonucleotides were tried to be used as primers in PCR for each species, we found that 9 primers (for E. globlus) leads polymorphism, 6 primers for E. citriodora, and 6 primers for Maruha Yu-kari.

Published
1997

2. Identification of Eucalyptus citriodora clones micropropagated in tissue culture.

Author

Kojima E, Izumi M, Tanabe T, Matsuda M, Shimizu T, Murakami A, and Murakami K

Subjects
Base Sequence, Cell Culture Techniques methods, Clone Cells, DNA Fingerprinting, DNA Primers, DNA, Plant genetics, DNA, Plant isolation & purification, Electrophoresis, Agar Gel, Plant Leaves, Polymerase Chain Reaction methods, Random Amplified Polymorphic DNA Technique, Templates, Genetic, Trees cytology, DNA, Plant chemistry, Trees genetics
Abstract

The extent of genetic identity observed in the young individuals which were micropropagated from a single Eucalyptus individual was analyzed by using DNA-fingerprinting. Among 40,000 tissue-cultured-seedings of E.citriodora, 200 plants were randomly chosen so that each total DNA might be extracted from their leaves. Using these DNAs as template, PCR was performed with some primers we found in advance that leads polymorphism for DNA of E. citriodora. In this study, all over the 200 cases, the band pattern formed cDNA fragment on a gel after electrophoresis was the identical one mutually.

Published
1997

3. Novel primers designed for microsatellite loci in Eucalyptus and identification by PCR fingerprints.

Author

Matsuda M, Kojima E, Izumi M, and Murakami K

Subjects
Base Sequence, DNA Fingerprinting methods, DNA Primers, DNA, Plant chemistry, Polymerase Chain Reaction methods, Species Specificity, Trees classification, Microsatellite Repeats, Trees genetics
Abstract

We found a novel PCR-primer which can be used for the identification on "elite-tree-selection". This primer was designed for selective hybridization at the both ends of microsatellite loci, which is well known as one of the most highpervariable region of DNA. After PCR-fingerprinting on five Eucalyptus species (E. globulus, E.citriodora, E.grandis, E. maidenii, E.bicostata), with our primer, DNA-polymorphism was observed all over the cases.

Published
1997

4. Synthesis and antiviral activities of 9-cyclopentylpurine derivatives.

Author

Kojima E, Ohta K, Miyawaki S, Date T, Machida M, and Murakami K

Subjects
Adenine chemical synthesis, Animals, Antiviral Agents chemistry, Avian Sarcoma Viruses drug effects, Chick Embryo, Drug Evaluation, Preclinical, Fibroblasts, HIV-1 drug effects, In Vitro Techniques, Stereoisomerism, Structure-Activity Relationship, Adenine analogs & derivatives, Adenine pharmacology, Antiviral Agents chemical synthesis, Antiviral Agents pharmacology
Abstract

The synthesis and anti-RSV (Rouse Sarcoma Virus) activity of HMCA, (+/-)-9-(2-hydroxymethyl-cyclopenthyl)-adenine, and its derivatives are described. It has been demonstrated that trans-HMCA has greater anti-RSV activity in tissue culture than cis-HMCA.

Published
1995

5. Study of JA300 as agent having high transglycosylation activity.

Author

Izumi M, Kojima E, Ishida S, Kaneko R, Miyawaki S, Kawaoka A, Machida M, and Murakami K

Subjects
Base Sequence, DNA Primers genetics, DNA, Bacterial genetics, Gene Expression Regulation, Bacterial, Gene Expression Regulation, Enzymologic, Genes, Bacterial, Glycosylation, RNA, Messenger genetics, RNA, Messenger metabolism, Species Specificity, Escherichia coli enzymology, Escherichia coli genetics, Purine-Nucleoside Phosphorylase genetics, Purine-Nucleoside Phosphorylase metabolism
Abstract

Escherichia coli JA300 as the strain catalyzing transglycosylation of nucleic acids has so strong activity. We scrutinized where the high faculty of this strain come from. Using PCR methods with our primer pair MI-1 (up; GTT GAA TTC GCC TTT GTT ATG TCA C) and MI-2 (down; GTT CCG CTA ACG GAA CAC CTA GGC CT), we isolated deoD (the coding region of PNPase) of JA300. Nucleotide sequence analysis indicated that deoD of JA300 is definitely same as that of parent strain K12. The fact that the transcription of deoD of JA300 is more active than that of K12 was also revealed after the analysis by Northrn blot hybridization. These data may suggest that the varieties of gene concerning regulation contribute to the difference of PNPase activity between them.

Published
1995