1. Supplementation with Red Wine Extract Increases Insulin Sensitivity and Peripheral Blood Mononuclear Sirt1 Expression in Nondiabetic Humans
- Author
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Yoshio Ogura, Daisuke Koya, Munehiro Kitada, and Itaru Monno
- Subjects
0301 basic medicine ,Blood Glucose ,Male ,THP-1 Cells ,red wine extract ,Wine ,Resveratrol ,AMP-Activated Protein Kinases ,resveratrol ,chemistry.chemical_compound ,0302 clinical medicine ,Sirtuin 1 ,Sirt1 ,Nutrition and Dietetics ,medicine.diagnostic_test ,food and beverages ,Alanine Transaminase ,gamma-Glutamyltransferase ,Middle Aged ,Female ,lipids (amino acids, peptides, and proteins) ,medicine.symptom ,lcsh:Nutrition. Foods and food supply ,Adult ,medicine.medical_specialty ,030209 endocrinology & metabolism ,Inflammation ,lcsh:TX341-641 ,Peripheral blood mononuclear cell ,Article ,03 medical and health sciences ,Young Adult ,Insulin resistance ,Internal medicine ,medicine ,Humans ,insulin sensitivity ,Aspartate Aminotransferases ,Triglycerides ,polyphenols ,Aged ,Triglyceride ,business.industry ,Interleukin-6 ,Lipid metabolism ,medicine.disease ,Lipid Metabolism ,030104 developmental biology ,Endocrinology ,chemistry ,Leukocytes, Mononuclear ,Insulin Resistance ,Lipid profile ,business ,Homeostasis ,Biomarkers ,Food Science - Abstract
The aim of this study was to investigate the effects of dietary supplementation with a nonalcoholic red wine extract (RWE), including resveratrol and polyphenols, on insulin sensitivity and Sirt1 expression in nondiabetic humans. The present study was a single-arm, open-label and prospective study. Twelve subjects received supplementation with RWE, including 19.2 mg resveratrol and 136 mg polyphenols, daily for 8 weeks. After 8 weeks, metabolic parameters, including glucose/lipid metabolism and inflammatory markers, were evaluated. mRNA expression of Sirt1 was evaluated in isolated peripheral blood mononuclear cells (PBMNCs). Additionally, Sirt1 and phosphorylated AMP-activated kinase (p-AMPK) expression were evaluated in cultured human monocytes (THP-1 cells). Supplementation with RWE for 8 weeks decreased the homeostasis model assessment for insulin resistance (HOMA-IR), which indicates an increase in insulin sensitivity. Serum low-density lipoprotein-cholesterol (LDL-C), triglyceride (TG) and interleukin-6 (IL-6) were significantly decreased by RWE supplementation for 8 weeks. Additionally, Sirt1 mRNA expression in isolated PBMNCs was significantly increased after 8 weeks of RWE supplementation. Moreover, the rate of increase in Sirt1 expression was positively correlated with the rate of change in HOMA-IR. The administration of RWE increased Sirt1 and p-AMPK expression in cultured THP-1 cells. Supplementation with RWE improved metabolism, such as insulin sensitivity, lipid profile and inflammation, in humans. Additionally, RWE supplementation induced an increase in Sirt1 expression in PBMNCs, which may be associated with an improvement in insulin sensitivity. more...
- Published
- 2020