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Trace elements (Mn, Fe, Co, Ni, Cu, Zn, etc.) are essential micronutrients for marine organisms and are thus related to major issues in the ocean, such as climate change, ecosystem change, and pollution. However, given their extremely low concentrations in seawater, contamination-free clean seawater sampling for trace elements onboard is the most crucial factor in this study. Because of the absence of both clean seawater sampling techniques and related infrastructure (e.g., a research vessel equipped with a nonmetal sampling system), the trace element community in Korea has not contributed to the global community studying trace elements in the ocean, although the associated global joint study program “GEOTRACES” has been operating for 10 years. With the newly launched research vessel (R/V) Isabu of the Korea Institute of Ocean Science and Technology (KIOST), Korea is now equipped with a large comprehensive R/V complete with a contamination-free PRISTINE ultraclean seawater sampling system for trace elements. We conducted clean seawater sampling 2 research cruises in the Indian Ocean during 2017–2018 aboard the R/V Isabu, and we successfully obtained reliable data, which agree well with previously reported trace element data at these locations. Here, we present the procedure and preliminary results of clean water sampling for trace elements based on the R/V Isabu, as the first Korean open ocean trace element study. From this study, we expect that R/V Isabu will play an important role in research on oceanic trace elements and isotopes in the future.

Chemical oxygen demand (COD) is a practical parameter that is used to estimate the amount of organic pollutants in aqueous systems. It is generally used as a guideline to control the quality of waste treatment effluent globally and is a management tool to evaluate the total pollution load in the highly developed coastal regions of Korea. It is a preferred method because of the speed and simplicity of the analysis and because there are fewer instrumentation requirements. The Ministry of Oceans and Fisheries (MOF) of the Republic of Korea developed a standard procedure for the measurement of COD. It has been revised several times, and the most recent revision was made in 2013 (MOF 2013–230). In this study, we modified the standard COD measurement procedure (MOF 2013–230), especially the sample digestion apparatus, to enhance analytical efficiency for a large sample number (batch), which is called a Korea Institute of Ocean Science and Technology (KIOST) modified MOF 2013–230. We examined uncertainty related to each experimental step and optimized laboratory conditions to reduce such uncertainties. The detection limit and estimated expanded uncertainty related to the KIOST modified MOF 2013–230 was 0.18 and 0.11 mg O2/L at a 95% confidence level (k = 2), respectively. This study also provides several tips to maintain consistent COD measurements in seawater using the alkaline potassium permanganate method.

We measured the community-scale metabolism of seagrass meadows in Bulgaria (Byala [BY]) and Korea (Hoopo Bay [HP]) to understand their ecosystem function in coastal waters. A noninvasive in situ eddy covariance technique was applied to estimate net O2 flux in the seagrass meadows. From the high-quality and high-resolution time series O2 data acquired over > 24 h, the O2 flux driven by turbulence was extracted at 15-min intervals. The spectrum analysis of vertical flow velocity and O2 concentration clearly showed well-developed turbulence characteristics in the inertial subrange region. The hourly averaged net O2 fluxes per day ranged from -474 to 326 mmol O2 m-2 d-1 (-19 ± 41 mmol O2 m-2 d-1) at BY and from -74 to 482 mmol O2 m-2 d-1 (31 ± 17 mmol O2 m-2 d-1) at HP. The net O2 production rapidly responded to photosynthetically available radiation (PAR) and showed a good relationship between production and irradiance (P-I curve). The hysteresis pattern of P-I relationships during daytime also suggested increasing heterotrophic respiration in the afternoon. With the flow velocity between 3.30 and 6.70 cm s-1, the community metabolism during daytime and nighttime was significantly increased by 20 times and 5 times, respectively. The local hydrodynamic characteristics may be vital to determining the efficiency of community photosynthesis. The net ecosystem metabolism at BY was estimated to be -17 mmol O2 m-2 d-1, which was assessed as heterotrophy. However, that at HP was 36 mmol O2 m-2 d-1, which suggested an autotrophic state.

In this study we describe measures taken in our laboratory to improve the long-term precision of nitrate and ammonia analysis in seawater using a microflow segmented-flow analyzer. To improve the nitrate reduction efficiency using a flow-through open tube cadmium reactor (OTCR), we compared alternative buffer formulations and regeneration procedures for an OTCR. We improved long-term stability for nitrate with a modified flow scheme and color reagent formulation and for ammonia by isolating samples from the ambient air and purifying the air used for bubble segmentation. We demonstrate the importance of taking into consideration the residual nutrient content of the artificial seawater used for the preparation of calibration standards. We describe how an operating procedure to eliminate errors from that source as well as from the refractive index of the matrix itself can be modified to include the minimization of dynamic refractive index effects resulting from differences between the matrix of the samples, the calibrants, and the wash solution. We compare the data for long-term measurements of certified reference material under two different conditions, using ultrapure water (UPW) and artificial seawater (ASW) for the sampler wash.

We have developed a new autonomous benthic lander for deep-sea research, the Korea Institute of Ocean Science and Technology (KIOST) BelcII and BelpII. The benthic lander was successfully tested at 950 and 1450 m water depths on the slope off the southwestern part of the Ulleung Basin in the East Sea of Korea. The ex situ measurements of the total oxygen uptake (TOU) rates at all the stations exceeded the in situ measurement values, and may indicate artificial effects from onboard incubation. The TOU rates were estimated to be 5.80 mmol m–2 d–1 and 3.77 mmol m–2 d–1 at water depths of 950 m and 1450 m, respectively. The benthic nutrient fluxes were also higher at water depths of 950 m, which indicates a partitioning of organic degradation with water depth. In addition, the negative phosphate and nitrogen benthic flux ratios and the higher nitrate removal flux via the sediment–water interface at the slope imply that the nitrogen in the bottom water may be preferentially removed via microbial respiration processes in the sediments, and may be coupled with the low nitrogen-to-phosphate ratio found in the deep water. Although our measurements comprised just two experiments in the slope sediment, the robust in situ measurement of the benthic fluxes in the slope sediment is a forerunner for new research into the biogeochemical cycles across the shelf edge–slope–basin system in the East Sea.

We have developed a novel miniature in situ microprofiler (NAFRI BelpI) for coastal and deep-sea studies. The BelpI can carry up to three oxygen microsensors, and it allows for simultaneous replicate measurements in a relatively small area. All functions can be controlled and programmed via a small magnetic bar and three reed switches; this feature facilitates the initial setup both onboard and underwater. The large LCD window shows basic information such as the movement intervals of the linear stage, number of replicate measurements, waiting time between measurements, and the pA from the microsensors. From high-resolution vertical profiles of oxygen in coastal sediment measured using the microsensors, the diffusivity boundary layer thicknesses was determined to be in the range 0.30–0.35 mm. In addition, the time-series measurement of oxygen profiles in permeable sandy sediment showed advective transport of oxygen into pore water by tidal agitation. Two examples of in situ measurement using a BelpI suggest that it can be widely applied to the study of the sediment-water interface.